ISSN:0741-0581    

DOI:10.1002/jemt.1060040302

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

摘要:

AbstractPyramidal tract (Pt) neurons in the sensory‐motor cortex of cat were labeled by injection of HRP into the spinal cord. Ultrastructural and quantitative analysis of the synaptic covering of their soma and apical dendrite (up to 100 μm from soma) was undertaken. We intercalated a visual‐manual treatment between composite electron micrographs and a fully automated computer system and developed specific programs for evaluation of the morphometric data. Programs are included. A total of 412 synaptic boutons were examined that were found in contact with large Pt neurons. The mean linear percentage of the surface area covered by boutons was 26.2 ± 8.4% and the mean contacting length and cross‐sectional area of the bouton profiles were 1.28 ± 0.58 μm and 0.89 ± 0.59 μm2, respectively. All types of boutons with active zones accounted for 41.2% of the total. The distribution of two types of bouton (S‐ and F‐type boutons, showing asymmetric and symmetric contacts, respectively) was examined quantitatively. The mean proportion of F‐type boutons was 89.1% with a soma and S‐type boutons contacting apical dendrites was 10.9%. In addition, GABAergic boutons were identified with the soma by immunocytochemistry with antibodies against glutamic acid decarboxylase. They formed symmetric synaptic contacts with the Pt cells that were identical to those formed by F‐type boutons. The quantitative analysis revealed that synaptic clefts are narrower and synaptic vesicles are smaller in symmetric F‐type boutons than in S‐type boutons forming asymmetric contacts. These data establish that at least three parameters (postsynaptic density, synaptic cleft, and size of vesicles) can be utilized singly or in combination to identify GABAergic inhibit

ISSN:0741-0581    

DOI:10.1002/jemt.1060040303

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

摘要:

AbstractA styrene‐based, low viscosity, negative‐staining resin has been formulated for low‐temperature embedding of unstained biological material. The resin, containing 3 atom% tin, permits sample preparations at 243°K, and provides sufficient contrast with bright field conventional transmission electron microscopy (CTEM) and excellent contrast in scanning transmission electron microscopy (STEM) rati

ISSN:0741-0581    

DOI:10.1002/jemt.1060040304

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

摘要:

AbstractElectron microscopy can aid in the rapid diagnosis of viral diseases, as it can be performed in a matter of hours, but on a routine basis it should be used in conjunction with other techniques. Initially, the specimen source and patient symptoms should be ascertained, as these will lend suggestions of possible agents while eliminating others; however, this information should not be allowed to prejudice observation in such a way as to cause oversight of an unlikely pathogen. Second, selection of the method of preparation should be based on sample consistency; extraction, debris clarification, concentration, tissue culture amplification, or embedment may be necessary. Finally, false‐positive results must be avoided by differentiating viruses from cell organelles or debris, mycoplasmal or bacterial contamination, and bacteriophage

ISSN:0741-0581    

DOI:10.1002/jemt.1060040305

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

摘要:

AbstractMethods for measuring and evaluating changes in volume of small tissue blocks prepared for transmission electron microscopy are presented. The results indicate: (1) that some blocks swell and others shrink, and (2) that changes in block volume can be explained by inhomogeneous changes occurring in three different tissue compartments.For stereological studies attempting to extrapolate changes in fixed and embedded tissue to those of fresh tissue, consequences of inhomogeneous volume changes in tissue compartments include a decrease in reliability and an increase in statistical variance of stereological density estimates. Since factors could not be found to correct for the changes in individual tissue compartments, alternative strategies are considered for dealing with the volume artifacts of fixation.

ISSN:0741-0581    

DOI:10.1002/jemt.1060040306

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

ISSN:0741-0581    

DOI:10.1002/jemt.1060040307

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY

ISSN:0741-0581    

DOI:10.1002/jemt.1060040301

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1986

数据来源: WILEY