摘要:

A reverse phase HPLC method for the quantitative determinations of alachlor [2-chloro-N-(2,6-diethylphenyl)-N-(methoxymethyl)acetamide] and its degradation products in alginate-based alachlor controlled release formulations has been developed. Sonication and the addition of sodium ethylenediaminetetraacetate as a chelating agent were employed to disintegrate the formulation matrix. The disintegrated formulations were extracted with acetonitrile. The acetonitrile extracts were analyzed for alachlor and its degradation products by HPLC. A C18column with a mobile phase of 65% acetonitrile and 35% water was used for separation. A UV detector set at 215 nm was selected for quantitation, and a photodiode array detector was used for confirmation. The developed method was used for the determinations of the percent active ingredient in freshly prepared and aged alachlor formulations made with and without oil (linseed, soybean, or corn oil). The method was also applied to monitor the rate of release and the fate of alachlor in controlled-release studies of alachlor formulations in water. Alachlor degradation products, N-demethoxymethylalachlor and 2,6-diethylaniline (trace), were found in the formulations with oil but not in formulations without oil. The degradation of alachlor continued slowly as the formulations aged.

ISSN:1082-6076    

DOI:10.1080/10826079608005526

出版商:Taylor & Francis Group    

年代:1996

数据来源: Taylor

摘要:

This method employs a ternary gradient generated from (1) a 90:10 water:methanol solution that is 0.015Min potassium phosphate at pH 5.1; (2) methanol; and (3) acetonitrile to separate fifteen explosives, byproducts, and metabolites of 2,4,6-trinitrotoluene (TNT) on a C18/anion exchange stationary phase. TNT, hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), six reduction metabolites and three oxidation metabolites of TNT have been separated and quantitated in a single chromatographic run. Because of the anion exchange character of the stationary phase, the anionic TNT metabolite 2,4,6-trinitrobenzoic acid also may be separated and quantitated using this method. Quantitation limits generally lower than 1 ppm have been established by a systematic certification procedure. The performance of the mixed mode separation method combined with diode array ultraviolet absorbance detection has been demonstrated with samples derived from both biological (tissues and physiological fluids) and environmental (soil, composts, and leachates) sources.

ISSN:1082-6076    

DOI:10.1080/10826079608005527

出版商:Taylor & Francis Group    

年代:1996

数据来源: Taylor

ISSN:1082-6076    

DOI:10.1080/10826079608005528

出版商:Taylor & Francis Group    

年代:1996

数据来源: Taylor

ISSN:1082-6076    

DOI:10.1080/10826079608005529

出版商:Taylor & Francis Group    

年代:1996

数据来源: Taylor