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11. |
Simultaneous Determination of Quinine and a Major Metabolite 3-Hydroxyquinine in Biological Fluids by HPLC Without Extraction |
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Journal of Liquid Chromatography & Related Technologies,
Volume 19,
Issue 2,
1996,
Page 293-305
S. Wanwimolruk,
S.M. Wong,
H. Zhang,
P.F. Coville,
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摘要:
A reverse phase, isocratic HPLC method has been developed for the quantitation of quinine and its major metabolite, 3-hydroxyquinine in human plasma, urine and hepatic microsomal samples. The method involves simple protein precipitation for sample treatment and ion-paired chromatography. The chromatographic separation is accomplished with a mobile phase comprising acetonitrile-aqueous phosphate buffer (40:60, v/v) containing 10 mM sodium dodecyl sulphate and 0.1 mM tetrabutylammonium bromide and adjusted to pH 2.1. The mobile phase is pumped at a flow rate of 0.5 mL/min. A microbore column is used (2 mm I.D. × 100 mm) packed with a C18reverse phase material (5 μm ODS Hypersil). Biological samples (100–500 μL) were precipitated with two volumes of cold methanol, vortexed and then centrifuged at 1500 g for 10 min. The supernatant (30 μL) was injected into the HPLC column. The chromatograms were monitored using a fluorescence detector setting with excitation and emission wavelenths of 350 and 450 nm, respectively. Under these conditions, the lower limit of detection was 0.1 μM (0.034 μg/mL) for the major metabolite 3-hydroxyquinine, and 0.5 μM (0.16 μg/mL) for quinine. The inter- and intra-assay coefficients of variation were found to be less than 7%. The assay procedure is applicable for studying the pharmacokinetics and metabolism of quinine.
ISSN:1082-6076
DOI:10.1080/10826079608005513
出版商:Taylor & Francis Group
年代:1996
数据来源: Taylor
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12. |
Perfluorooctyl and Perfluorobutyl Bonded Alumina Stationary Phases for High Performance Liquid Chromatography |
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Journal of Liquid Chromatography & Related Technologies,
Volume 19,
Issue 2,
1996,
Page 307-332
JeromeE. Haky,
TinaM. Blauvelt,
LarryF. Wieserman,
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PDF (1067KB)
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摘要:
The preparation and properties of perfluorooctylalumina (PFOA) and perfluorobutylalumina (PFBA) high performance liquid chromatographic stationary phases have been investigated. The PFOA phase was produced by chemisorption of perfluorooctanoic acid onto the surface of alumina. The PFBA phase was produced by a similar adsorption of perfluorobutylphosphonic acid onto alumina. Both phases exhibit reverse phase liquid chromatographic properties. Elemental analyses of these materials indicated that alkyl group surface coverage of the PFBA phase is higher than that of the PFOA phase. In contrast, retention of solutes on the PFBA phase is lower than that of PFOA. Isocratic capacity factors of over 20 compounds on the PFOA and PFBA phases were determined and compared with those obtained on octadecylalumina (ODA) and octadecylsilica (ODS) phases. In contrast to the greater retention of phenols than other compounds that was evident on the unfluorinated ODA phase, the retention of phenols on the PFOA and PFBA phases was not found to be significantly different from that of other compounds. These results are attributed to a reduced degree of hydrogen bonding interactions between phenolic solutes and the PFOA and PFBA phases compared to those which occur between phenols and the ODA phase. Preliminary investigations of the utilization of the PFOA phase for the separation of peptides and the employment of the PFBA phase for the rapid separation of phenols are also described.
ISSN:1082-6076
DOI:10.1080/10826079608005514
出版商:Taylor & Francis Group
年代:1996
数据来源: Taylor
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13. |
Liquid Chromatography Calendar |
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Journal of Liquid Chromatography & Related Technologies,
Volume 19,
Issue 2,
1996,
Page 333-337
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PDF (175KB)
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ISSN:1082-6076
DOI:10.1080/10826079608005515
出版商:Taylor & Francis Group
年代:1996
数据来源: Taylor
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