摘要:

An HPLC method for the determination of fenbendazole (FBZ) and its metabolites, fenbendazole sulphoxide (oxfendazole. FBZ-SO) and fenbendazole sulphone (FBZ-SO2) in trout and eel tissue is described. The compounds are extracted with ethyl acetate and the extract, after addition of hexane, is concentrated and cleaned up on a silica gel solid-phase extraction column. After elution with 3% acetic acid in methanol the reconstituted eluate is analysed on a Lichrosorb RP8 column, the mobile phase being 0.05 M ammonium phosphate (pH=5): acetonitrile:methanol (55:28:17,v/v). Detection is performed at 297 nm. The average recovery in trout muscle tissue over the concentration range 10–500 jig/kg is 75.7 ± 3.3 % and 77.5 ± 4.1 % for FBZ-SO and FBZ-SO2, respectively, and for FBZ the recovery is 76.3 ± 5.5 % over the range 15–750 μg/kg.

ISSN:1082-6076    

DOI:10.1080/10826079608014008

出版商:Taylor & Francis Group    

年代:1996

数据来源: Taylor

摘要:

A range of solid phase extraction (SPE) sorbents was investigated to determine their suitability as a pre-fractionation step during the quantitation of collagen crosslinks and advanced glycation end products by reverse phase high performance liquid chromatography (RP HPLC).

ISSN:1082-6076    

DOI:10.1080/10826079608014009

出版商:Taylor & Francis Group    

年代:1996

数据来源: Taylor

摘要:

The retention of 13 steroidal drugs was determined on a polyethylene-coated silica column using methanol-water mixtures at various organic phase concentrations. Good linear relationships were found between the log k' values and the organic mobile phase concentration in the eluent. The slope and intercept values of the relationships were correlated with the physicochemical parameters of the steroidal drugs by means of canonical correlation analysis. Calculation proved that the retention characteristics significantly depend on the physicochemical parameters of the solute. The electronic parameters exerted a higher impact on the retention than the hydrophobicity did. This result, is probably due to the hydrophobic interactions between the polar substructures of drugs and the free silanol groups not covered by the hydrophobic ligand.

ISSN:1082-6076    

DOI:10.1080/10826079608014010

出版商:Taylor & Francis Group    

年代:1996

数据来源: Taylor

摘要:

This is a scanned image of the original Editorial Board page(s) for this issue.

ISSN:1082-6076    

DOI:10.1080/10826079608013997

出版商:Taylor & Francis Group    

年代:1996

数据来源: Taylor