摘要:

An HPLC method for the simultaneous determination of paraquat and diquat in aqueous solutions and biological fluids (plasma, urine and vitreous humour) was developed. This method is based on the initial ion-pair solvent extraction of both herbicides from plasma or urine. Vitreous humour samples required a protein precipitation and concentration process. Relatively small sample volumes (1 mL of plasma or urine, and 100 μL of vitreous humour) were enough to determine paraquat and diquat by the proposed technique. Chromatography was carried out using a LiChrospher(r)100 RP-18 (5 μm) column for aqueous solutions and plasma and urine extracts, or a Nova-Pak C18(3.9 × 150 mm) column for vitreous humour extracts. Two ultraviolet wavelengths were selected, 254 nm for paraquat and 310 nm for diquat. The calibration curves were linear in the concentration ranges 0.42–8.4 μg/mL for aqueous solutions, 0.1–2 μg/mL for plasma, 0.1–3 μg/mL for urine and 0.5–5 μg/mL for vitreous humour.

ISSN:1082-6076    

DOI:10.1080/10826079608014022

出版商:Taylor & Francis Group    

年代:1996

数据来源: Taylor

摘要:

This is a scanned image of the original Editorial Board page(s) for this issue.

ISSN:1082-6076    

DOI:10.1080/10826079608014011

出版商:Taylor & Francis Group    

年代:1996

数据来源: Taylor