摘要:

A simple and rapid method was developed for the determination of amfepramone hydrochloride, fenprorex, and diazepam in capsules using high performance liquid chromatography (HPLC) with UV detection. This procedure provided conditions for the separation of the active ingredient from the complex matrices of the dosage forms by extraction in methanol. Isocratic reversed phase chromatography was performed using acetonitrile, methanol, and aqueous 0,1% ammonium carbonate (50:10:40) as a mobile phase, LiChrospher 100 RP 18 column (125 × 5 mm id, 5μm), a column temperature of 25 ± 1°C and detection at 230 nm.

ISSN:1082-6076    

DOI:10.1081/JLC-100101512

出版商:Taylor & Francis Group    

年代:2000

数据来源: Taylor

摘要:

Countercurrent chromatography (CCC) of proteins was performed by the cross-axis coil planet centrifuge (cross-axis CPC) using polyethylene glycol (PEG) - dextran biphasic systems. A pair of eccentric coil assemblies was mounted at two different positions (off-center and central) on the rotary frame. The best separation was obtained from a two-phase solvent system composed of 4.0% (w/w) PEG 8000 - 5.0% (w/w) dextran T500 in 5 mM potassium phosphate buffer (pH 7.0) and 3 M sodium chloride using the upper PEG-rich phase as the mobile phase. The off-center column position yielded substantially better peak resolution between lysozyme and myoglobin than that obtained from the central position under otherwise identical conditions.

ISSN:1082-6076    

DOI:10.1081/JLC-100101513

出版商:Taylor & Francis Group    

年代:2000

数据来源: Taylor