摘要:

Presented here is a simple approach to the application of HPLC/CE for the separation of complex mixtures. A two-dimensional HPLC/CE instrumental setup was assembled from commercially available equipment. Fractions of the effluent from the HPLC system are collected into microtiter plates with the aid of a microfraction collector which allows the collection of samples by time, drops, or external signal (peaks). The fractions are then dried under vacuum at room temperature in a special unit, reconstituted, and analyzed by CZE. Any size or type of HPLC or CE column can be used with no limitation on the amount of sample injected into the HPLC. Any CE detection procedure, such as LIF, MS, UV, or other, can be used. This setup is practical, simple, robust, and allows the separation of complex mixtures. Preliminary results show the utility of this system for the analysis of a mixture of two protein digests, cytochrome C and myoglobin.

ISSN:1082-6076    

DOI:10.1081/JLC-100101441

出版商:Taylor & Francis Group    

年代:2000

数据来源: Taylor

摘要:

This work demonstrates that the PCR products of the Cytomegalovirus (CMV) can be detected by capillary electrophoresis in uncoated capillaries using a zwitterionic buffer. Furthermore, dilution of the sample in phosphate buffer leads to stacking which improves the sensitivity by the ultraviolet detection without the need for sample desalting or cleanup.

ISSN:1082-6076    

DOI:10.1081/JLC-100101442

出版商:Taylor & Francis Group    

年代:2000

数据来源: Taylor

摘要:

Spherical, non-porous organo-silica materials in the submicron size range were synthesized by sol-gel processing and tested for capillary electrochromatography (CEC). The organo-silica spheres were prepared by copolymerization of alkyltriethoxy-silanes and tetraethoxysilane under ambient conditions. The synthetic approach provides silica spheres that are uniform in size (<10% R.S.D.) and contain organic moieties (e.g., C8) ready to be used as stationary phase for CEC, eliminating the step of stationary phase attachment after particle fabrication. The final particle size is controlled by the water concentration in the initial reaction mixture. The particles were characterized using transmission electron microscopy, gas adsorption, and FT-IR. Fast CEC separations with high efficiency can be achieved using the fabricated submicron particles.

ISSN:1082-6076    

DOI:10.1081/JLC-100101443

出版商:Taylor & Francis Group    

年代:2000

数据来源: Taylor