11. |
Countercurrent Chromatographic Separation of Proteins by Cross-Axis Coil Planet Centrifuge: Choice of Polymer Phase Systems and Revolution Speed |
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Journal of Liquid Chromatography & Related Technologies,
Volume 21,
Issue 11,
1998,
Page 1727-1736
K. Shinomiya,
Y. Kabasawa,
Y. Ito,
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摘要:
Countercurrent chromatographic separation of proteins by the cross-axis coil planet centrifuge was maximized by selecting the suitable polymer phase system and revolution speed. Polymer phase systems composed of polyethylene glycol (PEG) 1000 and several inorganic salts were examined to determine partition coefficient values (K) for various proteins. The overall results indicated that the polymer phase system composed of 12.5% (w/w) PEG 1000 and 12.5% (w/w) dibasic potassium phosphate yielded suitable K values for most proteins except for cytochrome C and apo-transferrin which may be separable with a solvent system composed of 12.5% (w/w) PEG 1000 and 24% (w/w) potassium citrate. A series of experiments with the PEG 1000 - potassium phosphate system under various revolution speeds revealed that the best separation was achieved at 850 rpm. The above optimized conditions may be applied to separations of other protein samples.
ISSN:1082-6076
DOI:10.1080/10826079808001255
出版商:Taylor & Francis Group
年代:1998
数据来源: Taylor
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12. |
High Performance Liquid Chromatographic Determination of Taxol and Related Taxanes from Texas Callus Cultures |
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Journal of Liquid Chromatography & Related Technologies,
Volume 21,
Issue 11,
1998,
Page 1737-1737
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ISSN:1082-6076
DOI:10.1080/10826079808001256
出版商:Taylor & Francis Group
年代:1998
数据来源: Taylor
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13. |
Basic Principles of HPLC and HPLC System Troubleshooting |
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Journal of Liquid Chromatography & Related Technologies,
Volume 21,
Issue 11,
1998,
Page 1739-1739
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PDF (48KB)
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ISSN:1082-6076
DOI:10.1080/10826079808001257
出版商:Taylor & Francis Group
年代:1998
数据来源: Taylor
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14. |
Liquid Chromatography Calendar |
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Journal of Liquid Chromatography & Related Technologies,
Volume 21,
Issue 11,
1998,
Page 1741-1747
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PDF (275KB)
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ISSN:1082-6076
DOI:10.1080/10826079808001258
出版商:Taylor & Francis Group
年代:1998
数据来源: Taylor
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