ISSN:1784-3286    

DOI:10.1080/17843286.1974.11716903

出版商:Taylor&Francis    

年代:1974

数据来源: Taylor

ISSN:1784-3286    

DOI:10.1080/17843286.1974.11716904

出版商:Taylor&Francis    

年代:1974

数据来源: Taylor

摘要:

SummaryMeasurement of enzyme activity is based on proportionality between reaction rate and enzyme concentration.Possible causes of error are analysed : artefacts, presence of inhibitors in the substrate or the enzyme preparation, effects of temperature and pH, activator ions.In some cases evaluation of the saturating concentration in substrate is difficult or impossible.

ISSN:1784-3286    

DOI:10.1080/17843286.1974.11716905

出版商:Taylor&Francis    

年代:1974

数据来源: Taylor

摘要:

SummaryThe author draws one's mind on the danger of using ready for use reactives kits.Even if their easy use is undeniable, they are not always reliable.

ISSN:1784-3286    

DOI:10.1080/17843286.1974.11716906

出版商:Taylor&Francis    

年代:1974

数据来源: Taylor

摘要:

SummaryWe describe our experience about the use of commercial control serums during the determination of theα-amylase and alkaline phosphatases activities on the Technicon autoanalyzer ; we show the sources of error one can make when LDH and CPK activities are measured by means of commercial kits and we expose the danger of making use of a temperature correction factor illustrated by the determination of the SGPT on the LKB 8.600 at 35°C. Preference is given to the notification of the results in mU/ml at the working temperature.

ISSN:1784-3286    

DOI:10.1080/17843286.1974.11716907

出版商:Taylor&Francis    

年代:1974

数据来源: Taylor

摘要:

SummaryThe use of the determination of the gamma-glutamyl-transpeptidase (GT), the 5’nucleotidase and of the isoenzymes of the alkaline phosphatase for the differential diagnoses of liver disease is discussed.It is concluded that the GT is a useful test to diagnose diseases of liver, common bile duct or pancreas, but without distinction between these illnessess. An affection of these organs is not very probable if the GT is normal, it is very probable if the level of activity exceeds 500 units. In cancer patients without jaundice, a normal level of this enzyme suggests the absence of metastases of the liver. The enzymatic activity is normal in case of bone affection but can be elevated in case of some neurological diseases and 3 to 4 days after the beginning of a myocardial infarction.An abnormally elevated 5’nucleotidase accompanied with an elevated alkaline phosphatase suggests strongly the presence of an hepatobiliary damage, except when the alkaline phosphatase is very elevated, which may cause an“apparent”increase of the 5’nucleotidase and except when the elevation is due to a bone damage. The rise in case of bone damage seems however not to take place by more than 10 % of the cases. This enzyme is also useful for the diagnoses of active chronic liver damages.The determination of the isoenzymes of the alkaline phosphatase seems especially useful in case of an increase of the phosphatase alkaline whilst the 5’nucleotidase and the liver tests are normal. It often gives the possibility to determine whether the increase of the alkaline phosphatase in that case can be ascribed to a bone or to a liver damage. The technical details are important. The electrophoresis on acrylamide and the inactivation by heat seem to be adequate methods.To the autors it appears that the determination of the GT and of the 5’nucleotidase can find place in the classical liver tests, from one could eliminate other tests such as the Hanger. As far as the determination of the isoenzymes of the alkaline phosphatase is concerned, their application could be reserved for selected cases.

ISSN:1784-3286    

DOI:10.1080/17843286.1974.11716908

出版商:Taylor&Francis    

年代:1974

数据来源: Taylor

摘要:

SummaryHypergel cellulose acetate is an excellent medium for LDH-isoenzymes electrophoresis because of its high degree of gellation. The cellulose acetate is brought on a polyester sheet which makes it much easier to handle throughout the electrophoresis and coloration procedures. For coloration of the isoenzymes, the generally used techniques are applicable.

ISSN:1784-3286    

DOI:10.1080/17843286.1974.11716909

出版商:Taylor&Francis    

年代:1974

数据来源: Taylor

摘要:

SummaryThe electrophoreticβ-hexosaminidase pattern of Tay Sachs fibroblasts shows a large fraction, which correspond to the B fraction of normal material and a second fraction, which has the same electrophoretic mobility as the third fraction found in normal fibroblasts.The combined data of qualitative enzyme assays before and after heat inactivation and the electrophoretic pattern seem to give a reliable approach to the problem of detection of homozygote and heterozygote patients.

ISSN:1784-3286    

DOI:10.1080/17843286.1974.11716910

出版商:Taylor&Francis    

年代:1974

数据来源: Taylor

摘要:

SummaryThe authors have looked for fever and infections in a hundred consecutive cases treated in a medical intensive care unit. These patients suffered from severe intoxication, respiratory insufficiency, myocardial infarction, neurological diseases or endocrine disorders. Sixty-seven patients had fever, due to infection in at least 41 cases, 35 of which had a respiratory infection. Most of the patients with non-infectious fever had a myocardial necrosis or a cerebral affection. Twenty-seven patients acquired an infection within the unit ; these hospital-acquired infections are mainly due to fungi and to Gram-negative organisms. Many factors contribute to contaminate the patients : venous and urinary catheters, artificial ventilation, coma, spontaneous hypothermia, antibiotics, corticoids, etc. The main principles of the prevention of infections in intensive therapy units are recalled.

ISSN:1784-3286    

DOI:10.1080/17843286.1974.11716911

出版商:Taylor&Francis    

年代:1974

数据来源: Taylor

ISSN:1784-3286    

DOI:10.1080/17843286.1974.11716913

出版商:Taylor&Francis    

年代:1974

数据来源: Taylor