摘要:

AbstractOncocytoma, a benign solid tumor, occurs in a number of organs but most frequently in the kidneys. Its oncocytes display an intensely eosinophilic cytoplasm due to presence of numerous mitochondria. Mitochondrial DNA analysis of six renal cell oncocytomas and adjacent renal tissue was performed using five restriction endonucleases. In theHinfl restriction pattern, an additional 40 bp band was noted. This new band was demonstrated in all oncocytomas but in none of the corresponding renal tissues or additionally tested renal carcinomas. By reisolating and hybridizing this band to the mitochondrial genome, its sequence was localized within the cytochrome c oxidase subunit I gene. The data suggest that a molecular alteration of mitochondrial DNA is specific for oncocytomas, and this constitutes the first observed mitochondrial DNA change in a human solid tumor.

ISSN:1045-2257    

DOI:10.1002/gcc.2870010112

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractThe biological basis for acute leukemia associated with mediastinal germ cell tumors has remained unexplained due to lack of critical data that would illuminate the genetic relationship between the two tumors in a given patient. Here we present results of serial cytogenetic investigations on the pretreatment and posttreatment mediastinal yolk sac tumor and immature teratoma biopsies and two separate leukemic bone marrow aspirates from a patient who developed acute nonlymphocytic leukemia 11 months after the initial diagnosis of the germ cell tumor. Presence of an i(12p) in all tumor clones and trisomy 21 in one clone in the posttreatment mediastinal tumor and all leukemic clones establishes the common origin of all tumor clones and shows that in this case the leukemia was derived from the malignant germ cell clone.

ISSN:1045-2257    

DOI:10.1002/gcc.2870010113

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractThe human cellular oncogeneMYBhas been mapped to 6q22–q23. Deletions and translocations involving this region of the long arm of chromosome 6 occur frequently in human malignant melanoma, and there are anecdotal reports ofMYBgene rearrangements in this cancer. In the current study, Southern blotting and pulsed field gel electrophoresis (PFGE) have been performed to determine whetherMYBor its flanking regions are commonly altered in malignant melanoma. Southern blotting failed to document obvious rearrangement of theMYBgene in 15 cases studied. To extend analysis of theMYBregion, a long‐range restriction map was established by PFGE. This map was then linked to the known restriction map of frequent cutting enzymes. Based on the mapping data and analysis of theMYBregion in melanomas,Clal tissue‐specific variation due to methylation was demonstrated. Also, two melanomas (containing alterations in band 6q13) also demonstrated by PFGE a unique restriction fragment for theMYBgene. These results extend significantly the physical map surrounding theMYBlocus and provide further evidence for the rearrangement of chromosome 6 in malignant mel

ISSN:1045-2257    

DOI:10.1002/gcc.2870010114

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractDNA was prepared from tumour and normal tissue from 48 patients representing all common histological types of nonsmall‐cell lung cancer. Using eight DNA probes, which detect nine restriction enzyme fragment length polymorphisms (RFLP) on chromosome 3, we established that among the 44 informative patients 32 had lost alleles on the short arm of one of their copies of chromosome 3. Of these 32, at least 13 had also lost alleles on the long arm of chromosome 3, suggesting that the whole chromosome might be lost. For one patient, cytogenetic analysis indicated that the mechanism of allelic loss was reciprocal translocation followed by chromosomal loss of one of the reciprocal products. Two patients with allelic loss distal to the D3S3 locus (which maps to 3p13–14) retained heterozygosity at that locus. These results indicate that loss of alleles on the short arm of chromosome 3 is a common event in lung tumours of the nonsmall‐cell type, that this loss occurs by a variety of chromosomal mechanisms, and that the minimally deleted region is 3p13–1

ISSN:1045-2257    

DOI:10.1002/gcc.2870010115

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractWe report the sublocalization of the breakpoint in chromosome 22 in 33 patients with chronic myeloid leukemia (CML) who also had unusual marrow cytogenetics. In 23 patients, the leukemic clones were characterized by Philadelphia (Ph1) chromosomes that arose through complex translocations that involved three or more chromosomes. In the remaining ten patients, there were no detectable Ph1chromosomes despite molecular evidence for the presence of rearrangements in the major breakpoint cluster region (bcr) of chromosome 22 in all cases. There was no significant difference between the two groups with respect to location of the breakpoints within thebcr.When these two groups of patients were combined, there was a significant excess of breakpoints in one segment of thebcrwhen compared to the distribution of breakpoints seen in 119 patients with simple 9;22 translocations. The difference in breakpoint distributions did not appear to be entirely attributable to differences between groups in disease duration at the time of study. These data support the notion that the unusual genetic recombinations that give rise toBCR/ABLfusion genes in CML involve specific DNA sequences ofBCR(and possiblyABL) and additional, recombinogenic sequences, at least some of which are present in loci known to be nonrandomly involved in complex Ph1translocations.

ISSN:1045-2257    

DOI:10.1002/gcc.2870010116

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

摘要:

AbstractA case of acute nonlymphocytic leukemia with homozygosity for the chromosome segment 8pter→8q22 is reported. A t(8;21)(q22;q22) translocation was associated with duplication of the derivative chromosome 8q – and absence of the normal chromosome 8. These rearrangements also yielded hemizygosity for 8q22→qter. This case shows that acquired hemizygosity and homozygosity do exist in leukemia as in solid malignant t

ISSN:1045-2257    

DOI:10.1002/gcc.2870010117

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY

ISSN:1045-2257    

DOI:10.1002/gcc.2870010101

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1989

数据来源: WILEY