摘要:

Summary.Serial semithin sections of human testicular biopsy material were used for three‐dimensional reconstruction in order to obtain information about Sertoli cell nuclei in normal and pathologically altered seminiferous epithelia. The three dimensional reconstruction program is based on the triangulation of image point series. It includes a calculation modus for determining surfaces and an approximation formula for the estimation of volumes.Nuclei from the following specimens were reconstructed and for each the volume (v), surface (s), and a quotient (v/s), which is regarded as a marker for the degree of membrane infoldings, were calculated as follows: (1) For normal spermatogenesis (stage 1, 2, 3, 5;n=18) v = 409.7 ± 33.0 μm3, s = 429.6±40.5 μm2, v/s = 0.96 ± 0.05. The values revealed no stage‐specific differences; (2) for hypospermatogenesis (n=9) v = 493.7 ± 33.7 μm3, s = 462.6 ± 51.4 μm2, v/s = 1.07 ± 0.085; (3) for spermatogenic arrest at the level of primary spermatocytes (n= 4) v = 537.8 ± 49.5 μm3, s = 424.4 ± 28.7 μm2, v/s = 1.27 ± 0.052; (4) for spermatogenic arrest at the level of spermatogonia (n= 17) v = 472.4 ± 110.3 μm3, s = 397.8 ± 64.2 μm2, v/s = 1.18 ± 0.106; (5) for Sertoli‐Cell Only Syndrome (n=14) v = 438.7 ± 42.6 μm3, s = 345.1 ± 38.0 μm2, v/s = 1.28 ± 0.087; (6) for hypoplastic tubules in a biopsy showing mixed atrophy (n=19) v = 275.6 ± 53.2 μm3, s = 228.8 ± 26.0 μm2, v/s=1.20±0.115; (7) for ovot‐estis (46xx, 47xxy) (n=14) v = 417.3±71.2 μm3, s = 317.2±26.3 μm2, v/s= 1.31±0.13 and (8) for carcinomain situ(n=25) v = 436.3±83.1 μm3, s = 338.2±64.4 μm2, v/s = 1.30±0.08. A significant increase of nuclear volume was found in Sertoli cells of all specimens showing different types of spermatogenic impairment (maturation arrest, hypospermatogenesis) and a significant decrease in Sertoli‐cell nuclei from hypoplastic tubules. Compared to normal, v/s was significantly increased in all specimens showing pathological alterations suggesting that developmental and/or functional Sertoli cell defects seem to be co

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02732.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.The ultrastructural appearance of multinucleated spermatocytes in the aging human testis is documented emphasizing their mode of formation and their degenerative fate. Multinuclearity results from confluence of cell membranes of neighbouring spermatocytes of one clone. Intercellular bridges are not causally involved in this process but are only secondarily detached from the cell membrane and, attaining an intracellular position, subsequently disintegrate. Accompanying or following the process of formation of the multinucleated spermatocytes, these cells undergo degeneration. There are no indications of a regular further development of multinucleated spermatocytes. Though quite rarely found within the aging human seminiferous epithelium, the appearance of these cells represents a germ‐cell loss at the spermatocyte stag

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02733.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Book reviewed in this article:W. Cyran, M.J. Halhuber:Erotik und Sexualität im AlterFischer, Stuttgart, 1992, 103 pages, ISBN 3–437‐00707‐6, DM 19,80.L. Aresin:Sexualberatung durch den HausarztFischer, Stuttgart, 1992, 208 pages, 20 figures, ISBN 3–437‐00675

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02734.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.In this note new consequences of the Kartagener's syndrome are described. In males the syndrome involved a diffused sperm immaturity; in one female severe skeletal defects were present probably resulting from bad organization of the renal apparatus.

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02735.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.In this note several cases of stunted tails involving the total sperm population in sterile humans are described. Half of the cases are classified as ‘short tailed’ spermatozoa, the other half as ‘stump defect’ previously described in bulls. Both defects are referred in details at electron microscopica

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02736.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02737.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.To improve artificially the quality of poor sperm samples to be used in assisted procreation procedures, Test Yolk Buffer (TYB) incubation effect was investigated. After adding the TYB procedure to the standard swim‐up technique, a significant (P<0.001) increase in spermatozoa velocity was observed, while the other seminal parameters were not affected.Three of 11 couples who had not had success after three intraperitoneal inseminations (IPI) with sperm prepared by the usual swim‐up technique obtained pregnancies after IPI with TYB‐incubated spermatozoa.These preliminary results suggest that TYB incubation for capacitated spermatozoa be employed in the assisted procreation strategies applied for male infert

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02738.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.The motility characteristics of spermatozoa from asthenozoospermic semen were investigated and compared to the same parameters in fertile semen. The motility characteristics assessed by the CellSoft semen analyser (CRYO Resources Ltd, NY) were the following: curvilinear velocity (VCL), straight line velocity (VSL), amplitude of lateral head displacement (ALH), linearity (%LIN), and beat cross frequency (BCF).Analysis of the data indicated a decreased kinetic activity in the spermatozoa from the astheno‐zoospermic group which is expressed as a highly significant decrease (P<0.002) in the VCL and VSL compared to velocities from normospermic samples. Moreover, percentage linearity and ALH were also statistically lower (P<0.05) in this group. However, no difference was evidenced for the BC

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02739.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.With the aim of explaining some of the controversy on the value of computer‐assisted methods of semen analysis, a study was conducted comparing the fully automated cell motion analyzer (CMA), the semi‐computerized autosperm system (AS) and the conventional manual method. The CMA equipment gave higher values for sperm concentration than the mean of the three methods. Sperm concentration estimated with the conventional method was below the average of the three methods. The conventional method overestimated sperm motility, which was underestimated by CMA.The correlation between sperm velocity characteristics estimated by CMA and AS was significant, but only 18% of variability of one method could be explained by variability of the other. The reasons for the discrepancy between velocity measurements in the two methods are discussed in relation to possible shortcomings in sperm paths smoothing using the CMA method and the effect of technician training in the AS method.The AS method was found to provide more reproducible results which correlated better with those of the manual method as recommended by the World Health Organizat

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02740.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Fifty‐nine men who requested vasectomy and 43 infertile patients had a semen analysis performed prior to surgery or during evaluation. A hypo‐osmotic swelling test (HOS) and a new ‘Water test’ were performed simultaneously, in order to assess correlation between these two procedures. Our results showed that values obtained with the ‘Water test’ were significantly higher than those obtained with the HOS test (P<0.001). These findings suggest that it is necessary to determine normal values for this new test before introducing it in the routine sem

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02741.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY