摘要:

SummaryThe human acrosome, especially the acrosome reaction, is an important parameter for evaluation of sperm function. By histochemical staining, the acrosome is visualized at the light microscopic level. However, a high number of cells are lost due to the multiple centrifugation steps that are required for preparation of spermatozoa. In the present study, semen samples from oligozoospermic patients were stained by an improved method. Compared to the conventional triple‐stain technique, optimal staining of the acrosome and a lower number of cells lost during sperm preparation were observed (P<0.025). Differences in the percentage of live acrosome‐reacted spermatozoa were not statistically signific

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1995.tb01101.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummarySerum α‐immunoreactive inhibin was evaluated in 16 male renal transplant recipients with stabilized renal function of mean age 38.5±10.7 years and was compared to that in sex‐ and age‐matched 17 haemodialyzed patients; 10 patients with chronic renal failure and 26 healthy normal men (controls). Serum LH, FSH, prolactin and testosterone were also measured simultaneously, in the same samples. In renal transplant recipients, inhibin was found significantly higher than that in controls (P<0.001), 25% however of the values were in the normal range. In contrast, all haemodialyzed patients showed highly elevated inhibin values (P<0.0001). In patients with chronic renal failure values of inhibin were also highly elevated compared to those in normals. A negative correlation was noticed between inhibin and FSH only in controls (rs= ‐0.610,P<0.005). In renal transplant recipients, inhibin showed a strong correlation with serum creatinine (sCr) (rs= 0.710,P<0.008), while in the case of graft rejection the rise of sCr was immediately followed by a parallel increase of inhibin. In conclusion, the highly elevated inhibin in uraemic men not improved by haemodialysis and persistent after successful renal transplantation, might be implicated in the pronounced hypergonadotropic hypogonadism of haemodialyzed patients and the remaining dysfunction of hypothalamic‐pituitary‐testicular axis in renal transpla

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1995.tb01102.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryThe response of testicular macrophages to massive Leydig cell death was studied by the administration of the specific Leydig cell cytotoxic ethylene dimethane sulphonate (EDS) to sham‐operated (SO), short‐term (STHX), and long‐term (LTHX) hypophysectomized rats. EDS‐killed Leydig cells showed the morphological features of the programmed cell death or apoptosis. A 2‐fold increase in the number of macrophages was found on days 1–2 after treatment in both SO and STHX rats, and dead Leydig cells were completely eliminated by day 3 after treatment. Otherwise, in LTHX rats, there was a delay in the increase in the number of macrophages, and EDS‐killed Leydig cells remained in the testicular interstitium for several days. These results indicate that the phagocytic capacity of the macrophage population was diminished in hypophysectomized rats, and particularly after long‐term

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1995.tb01103.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryConcern about the reproductive potential of long‐term survivors of acute lymphoblastic leukaemia (ALL) prompted an investigation into the impact of the disease on spermatogenic cells. Using rats as a model, histological, immunocytochemical and electron microscopic analysis was applied to investigate changes in the seminiferous epithelium. In rats transplanted with leukaemic cells at early puberty, degenerate primary spermatocytes and spermatids were prevalent within stage VIII tubules. Electron microscopically, step 8 spermatids showed acrosomal abnormalities and nuclear contour distortion. In the distorted step 9 spermatids, the microtubules of the manchette were abnormally oriented or deficient. Antitubulin antibody staining was reduced in elongating spermatids in the group transplanted with leukaemic cells at early puberty but was not observed in the older leukaemic group. Step 13 spermatids showed extracted chromatin and degenerate step 19 spermatids were occasionally found. Similar but less severe changes were seen in the group of rats transplanted with leukaemic cells at late puberty. We conclude that germinal cell damage induced by ALL is dependent on the developmental maturity of the seminiferous epithelium. The present findings are of particular importance when interpreting the impact of anticancer chemotherapeutics on germinal cells in patients with AL

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1995.tb01104.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryChromatin stability and DNA‐resistance to acidic denaturation was evaluated by acidic aniline blue and acridine orange staining of cat sperm from different regions of the epididymis. The results were related to conventional sperm parameters. The percentage of aniline blue‐stained spermatozoa (persisting histones) decreased significantly from the caput to the cauda region (31.8% and 7.8%, respectively;P<0.0001). The percentage of stained heads of cauda sperm was much lower in populations of morphologically normal forms than in those with abnormal forms (4.1% and 13.8%, respectively,P0.0001). With acridine orange fluorescence staining, 86.5% of cauda epididymal sperm were found with well‐condensed chromatin, stabile against acid‐induced denaturation. Chromatin stability increased significantly from the caput epididymal region (51.1%) to the cauda epididymal region (86.5%). The percentage of cauda epididymal sperm with normal condensed chromatin was neither linked to testicular sperm count, motility nor to age of the cats. The parameter of chromatin condensation and stability can be a valuable index of sperm quality, reflecting the possible disorders of spermatogenesis and epididymal sperm maturation, frequently observed in feline

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1995.tb01105.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryTo evaluate the effects of antisperm auto‐antibody‐bound sperm on the outcome ofin vitrofertilization‐embryo transfer (IVF‐ET), 160 infertile couples undergoing treatment byin vitrofertilization were recruited in this study. In the study group (11 couples, 15 cycles), the male partners were positive for antisperm autoantibodies determined by immunobead test (IBT). In the control group (149 couples, 152 cycles), the men had no such antibodies. The percentages of fertilization rate, cleavage rate and pregnancy rate of the study group and control group were 75.0±5.2% vs. 69.3±2.4%; 82.8±3.7% vs. 89.8±1.2% and 6.7% vs. 11.8%, respectively. There were no significant differences inin vitrofertilization outcome between both groups. The region, type and/or percentage of sperm‐bound antibodies also had no effect on thein vitrofertilization outcome. In conclusion,in vitrofertilization‐embryo transfer is not significantly affected by antisperm autoantibody‐bound sperm determined b

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1995.tb01106.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryThe possible physiological role of testicular oxytocin in testicular steroidogenesis was studied in immature rats. In 5‐, 9‐, and 25‐day–old animals one of the testes was injected with 20, 50, and 200 ng of oxytocin, respectively, then the contralateral gonad was removed. Rats were killed 1 h, 1 day or 7 days post‐surgery. In each age group studied intratesticular injection of oxytocin resulted in a significant increase in basal testosterone secretionin vitroand/or serum testosterone concentration. Data indicate that in immature rats oxytocin of testicular origin might act as a local stimulator of steroi

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1995.tb01107.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY