摘要:

Summary.It was suggested that although not related to the standard semen parameters the level of the acrosin enzyme system is related to the fertility potential in men. Recently a simple clinical assay for total acrosin level was recommended for routine semen analysis. The improved clinical assay was analysed on the freshly liquified semen of 198 Astheno‐teratozoospermic men and compared with the routine semen parameters including biochemical data and the ultrastructure of the acrosome. Only the sum of the per cent of live, motile, and normal‐shaped spermatozoa had positive significant and reasonably high correlation with the acrosin level (r= 0.382,P<0.0001). Each characteristic exhibits significant but low (<0.35) correlation. Similarly negative significant and reasonably high correlation was obtained between the acrosin level and the sum of the principle acrosomal malformations observed byTEM(r= 0.396,P<0.0001) while lower negative correlation was found only with agenesis or loss of the acrosome. Acrosin levels below 8.1 μIU 10−6cells were obtained in 4 specimens with above 80% round‐form associated with more than 95% of agenesis of the acrosome. The possible significance of the low correlation obtained between the acrosin levels and seminal plasma zinc levels, malformations in the acrosomal equatorial region, and the presence of white blood cells is also discussed. We concluded that the acrosin activity reflects an aspect of male fertility which is not diagnosed by the routine semen analysis or by the ultrastructure of the acrosome, and is therefore a useful diagnostic sperm p

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02683.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.In a solution containing less than 5% seminal fluid, human sperm has a tendency to attach its head on the smooth surface of an object. Based on such a phenomenon, a simple method was developed to fix sperm head on the surface of glass slide. This method was demonstrated by studying caffeine effect on the tail movement of human sperm in phosphate buffered saline. By analysing the images recorded on a videocassette, caffeine, a well known stimulator of human sperm motility, was shown to induce an immediate increase of sperm TBF (tail beat frequency) and the amplitude of TBF increase was found to be larger in sperm with lower basal TBF value. This method can provide a flock of head‐fixed motile sperm for a precise sperm tail movement assessment or for other purpose

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02684.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Peritoneal fluids (PFs) from spontaneous (n= 14) and gonadotrophin‐stimulated cycles (n= 20) were obtained during diagnostic laparoscopy and gamete intrafallopian transfer (GIFT) procedures, respectively. The effects of these fluids on the linear component of sperm motility and on the percentage of motile spermatozoa were studiedin vitroby objective motility assessments and compared to a control medium (B2‐Ménézo).Overall, the two types of PFs were found to have rather similar effects on the motility parameters studied. However, the fluids from hormonally‐stimulated cycles sustained motility better (i.e., sperm velocity and percentage of motile sperm) and in a rather constant manner as a function of time (narrower range distributions of the motility measurements). Furthermore, it was observed that under identical experimental conditions motility measurements depended not only on the type ofPFused but also on the sperm sample.These results suggest that assisted reproduction procedures in whichPFis the medium where the gametes eventually meet and interact, such as direct peritoneal insemination (DIPI) or peritoneal oocyte and sperm transfer (POST), could have different success rates if performed in spontaneous rather than in stimulated cycles. At the same time, our results may help to explain why different pregnancy rates were reported in different studies usingDI

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02685.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.The expression of human leukocyte antigens (HLA) on ejaculated spermatozoa and on lymphocytes was compared by flow cytometry using monoclonal antibodies towards HLA class I (pan‐HLA‐A, ‐B, ‐C) and class II (DR) antigens. Soluble antigens of HLA class I (s HLA‐A, ‐B, ‐C) in seminal plasma and in blood plasma were monitored with anelisatechnique. Lymphocytes showed specific fluorescence after incubation with the antibodies against HLA class I and class II (DR), whereas, on spermatozoa no positive immunofluorescence could be detected. No antibodies were bound to any significant extent either after modifications of sperm preparation (density gradient centrifugation, swim up‐technique, addition of azide, foetal calf serum or benzamidine chloride) or after treatment of spermatozoa with detergens. Furthermore, different concentrations of soluble HLA‐A, ‐B, ‐C in seminal plasma and in blood plasma were detected. The latter one showed soluble HLA about four‐fold more concentrated than the seminal plasma (x ± SD: 262.5 ± 144.4 nmol 1‐1vs. 62.5 ± 27.1 nmol 1‐1). These results suggest, that the HLA‐expression differs between hum

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02686.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.According to the distribution of IMP, three different regions can be recognized on PF of the post‐acrosomal plasma membrane of bull, ram, and boar spermatozoa. They are: (1) a region with linear aggregation ofIMP, (2) a region with fewer and scatteredIMP, and (3) a region with more numerousIMP.In the last two regionsIMPsare randomly distributed or a clustering of certain particles is visible. In stallion spermatozoa the last two areas are undistinguishible.There are evident interspecies differences in the arrangement of linear aggregations ofIMPwhich are characteristic for each specie

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02687.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.A previous study demonstrated the establishment of a murine testicular antigen (mTA)‐specific CD4+ T‐cell line (designated BT.1) which was capable of transferring experimental autoimmune epididymo‐orchitis to naive recipient mice. The disease transfer was antigen‐specific, because no inflammatory lesion was observed in any other organs and tissues of the recipients. In this study, to investigate the local environment of BT.1 cells, the effect of the cells and their culture supernatant on a local tissue integrity was studied. WhenBT.1cells were seeded on cultured fibroblastoid cell monolayers, the cells completely disrupted these monolayers in spite of the absence of the specific antigens. Moreover, the culture supernatant ofBT.1cells induced non‐specific dermal inflammation when injected into skin tissue of normal syngeneic mice. Therefore,BT.1cells were shown to devastate a tissue integrity and cause attraction and activation of inflammatory cells of the recipient origin in a local environment. These results suggest that the transferredBT.1cells will specifically home to the testis and epididymis of recipients but the following devastation of seminiferous tubules and epididymal ducts might be non‐specifically produced by the inflammatory cells of both donor and recipient origin in

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02688.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Two monoclonal antibodies (MAbs) directed against human protamineP1were realized. Anti‐P1specificity was assessed by western‐blot and confirmed byelisa.Monoclonal antibody 97‐3 was selected. ProtamineP1, was specifically demonstrated in human testis by immunoelectron microscopy, using 97‐3MAband an indirect post‐embedding immunogold technique. Our results clearly demonstrated the precise time of appearance ofP1protamine in the nuclei of human spermatids.P1, first appeared in the nucleus of step 5 spermatids and its concentration was increased in steps 6–8 spermatids, cytoplasm was n

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02689.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Plasminogen activator activity (PAA) and plasminogen activator inhibition (PAI), against t‐PA (t‐PAI) or u‐PA (u‐PAI), in spermatozoa and seminal plasma as well as testosterone in the blood of Friesland, Chios, and Karagouniki rams all showed a seasonal variation with the highest values during the corresponding breeding season of the ewes (Autumn‐Winter). The seasonal variation of PAA and PAI in spermatozoa or seminal plasma as well as blood testosterone was different among the three breeds studied. Increased spermatozoal PAA was observed in November and May in Friesland rams, in October and November in Chios rams, and in October in Karagouniki rams. Spermatozoal t‐PAI was increased in December and June in Friesland rams, in November and December in Chios rams, and in November in Karagouniki rams. Spermatozoal u‐PAI was increased in December in Friesland rams, in October and December in Chios rams, and in November and December in Karagouniki rams. Plasminogen activator activity and PAI in seminal plasma also showed similar seasonal variations. Plasminogen activator activity and PAI in spermatozoa and seminal plasma showed a positive correlation with blood testosterone. The results of the present study support our previous findings on the possible role of spermatozoal PAA and PAI in the fertilizing ability o

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02690.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02691.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02692.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY