摘要:

Summary.Several types of sperm antigenic suspensions as well as the whole sperm, either methanol‐fixed or air‐dried, were checked for intensity of binding to monoclonal antisperm antibodies with known characteristics of reactivity to sperm. The activity of sperm antigen—antibody binding was measured by elisa (enzyme‐linked immunosorbent assay) and compared in several variations (parallely run) of the assay where different types of sperm antigen preparations were applied. The obtained results were then evaluated for statistical significance in Wilcox test. It was shown that antibody reactivity was markedly higher in experiments where the whole sperm was coated in a solid‐phase in comparison to results obtained with adhered different sperm antigenic suspensions. However, one exception was noted, where the results from elisa, run with sperm organic extract, were (statistically) insignificantly lower than those obtained with the whole sperm. Therefore, organic sperm extracts (containing mostly glycolipids) can be a valuable alternative to screening for antisperm antibody activity and/or infertility b

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02706.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Systems of computer‐assisted image processing (CAIP) offer good reproducibility and low intra‐assay variability between replicate analyses of the same sample. The correlation between these values and those obtained by directly microscopic observation can be well demonstrated. The comparability between systems of different manufacturers is sufficient and satisfactory. Thus, they are well‐suited for the routine work in the andrologic laboratory.Since sperm parameters are used for the prediction of male fertility, it is important to know whether computer‐assisted analysis improves the predictive power as compared to visual estimation. In our study we compared the variation of semen parameters between two semen samples of the same individual taken in an interval of at least 3 months, by using the CAIP and visual methods.Significantly close correlations between the two values were obtained by both methods. Values of cell concentration showed the highest correlation in both methods (r=0.75). The lowest correlation, but also statistically significant, was observed in the values of locally motile cells (r= 0.54 for CAIP andr=0.30 for visual determination). Although apparantly higher in CAIP within method correlation coefficients did not show statistically meaningful differences between both methods.We conclude from our study that if values of sperm concentration and motility as measured by CAIP are compared to those obtained by visual measurement, the first method is not superior to the latter in terms of retest reli

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02707.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.Spermatozoon motility is an important semen parameter that can be correlated with fertilizing capacity. It can be evaluated by subjective or objective methods such as the multiple exposure photography technique (MEP). This technique was: (1) validated in terms of accuracy and sensitivity, and (2) used to investigate the possibility of differentiating patient subgroups on the basis of sperm motility. The method was shown to be accurate. The within and between assay variation coefficients were less than 6% and 17% respectively. Maximal sensitivity required 100 spermatozoa, since coefficients of variation were high when counting smaller numbers.A significative difference between fertile, normozoospermic and asthenozoospermic groups on the basis of the population pattern was found in a retrospective study. Moreover, the asthenozoospermic group could be divided into three subgroups, depending on whether one, two, or three motility variables were altered.

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02708.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.During the course of sterility treatment semenograms of 271 IVF and 316 insemination patients were carried out by two automated semen analysers. The parameters of these analyses were correlated to pregnancies resulting from the treatment. Semen samples were analysed in the ejaculate and after swim‐up preparation. In addition, the swim‐up suspension of IVF patients was measured again 18 h after sperm preparation. Patients were divided into three groups: (1) couples who achieved a pregnancy, (2) couples who did not achieve a pregnancy, and (3) IVF patients with no fertilization of the oocytes. Because of large standard deviations in the quality of ejaculates, the results in the IVF group show no significant differences in the semen parameters of husbands of pregnant and non‐pregnant women. In contrast husbands of women with no fertilization have a significantly lower sperm motility. After swim‐up preparation these differences no longer occur. A further measurement, taken 18 h later, reveals that there are no differences in the sperm parameters between the pregnant and non‐pregnant group. However, the semen quality in the group with no fertilization is significantly reduced. The results of the insemination patients are similar to those of the IVF group. Thus, the results from automated sperm analysers cannot replace either the microscopic or biochemical analysis of an ejaculate and, moreover, cannot be used as prognosis for the fertilization capacity of sperms or a following

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02709.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.HS‐63 monoclonal antibody was shown to react with a sperm‐specific acrosomal antigen from spermatozoa of a variety of mammalian species. Indirect immunofluorescence showed that HS‐63 was associated with acrosome‐intact sperm after sperm had been fixed with methanol. Electron microscopy (EM) was employed to determine the ultrastructural localization of this sperm antigen. When the immunogold labelled goat anti‐mouse IgG was used as a probe, we demonstrated that HS‐63 monoclonal antibody did not bind to the freshly prepared human spermatozoa. However, gold particles were observed in the intra‐acrosomal region, when the spermatozoa had been pre‐treated with 0.5% Triton X‐100 prior to incubation with HS‐63. We further observed that the immunogold did not stain the inner acrosome membrane when the spermatozoa became acrosome‐reacted. A good correlation was obtained between the percentage of spermatozoa which did not react with HS‐63 as determined by indirect immunofluorescence assay and that of acrosome‐reacted spermatozoa as quantitated by electron microscopy. The results of this EM study were consistent with those obtained by indirect immunofluorescence assay and both indicated that HS‐63 reacts only with the capacitated and acrosome‐intact spermatozoa. Therefore, HS‐63 monoclonal antibody is a useful probe for rapid evaluation of acroso

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02710.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.The possible physiological role of testicular corticotropin‐releasing factor (CRF) in the regulation of testicular functions was studied in neonatal rats. Two microlitres of anti‐CRF‐antiserum (dilution: 1: 10 or 1: 100) was injected intratesticularly to 5 d‐old rats with two testes and to hemicastrates. Five days after hemicastration and treatment of the remaining testis with the antiserum, serum testosterone concentration and basal testosterone secretionin vitrodecreased significantly. Unilateral testicular injection of a‐CRF in rats with two testes resulted in a significant drop in serum testosterone level with no change in basal testosterone production. Data indicate that in neonatal rats testicular CRF might be a local stimulator of steroi

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02711.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.The components of the extracellular matrix, laminin and aminoterminal propeptide of type III procollagen (PIIINP) were determined in seminal plasma of 50 patients with vasectomy and of 50 age‐matched fertile patients. The concentration of laminin was highly significantly (P<0.001) elevated in the fertile group as compared to the vasectomy group, whereas the concentrations of PIIINP were not significantly different between these two groups. Only weak correlations were observed between the concentrations of laminin and PIIINP. It is suggested that part of the laminin found in seminal plasma is derived from the ductus deferens, while the source of PIIINP is probably located at an upper part of the urogenital trac

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02712.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Summary.The ability of intact cells to reduce spin labels has been utilized to characterize the activity of spermatozoa of goat. The kinetics of reduction of TEMPO has been found to be sensitive to the quantity, quality and state of epididymal maturation of the spermatozoa. Presence of alcohol caused activation and Gossypol acetic acid left sperm activity unaltered. Electron spin resonance (ESR) and31P Nuclear magnetic resonance (NMR) indicate that the period ofin vitrocapacitation requires optimization.31P NMR spectra indicate a good correlation with the progressive maturation of the cells.

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02713.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02714.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0303-4569    

DOI:10.1111/j.1439-0272.1993.tb02715.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY