摘要:

Abstract—By using a combination of subcutaneous and intraventricular injections of [14C]uridine and [3H]methyl‐l‐methionine we have obtained maximum incorporation in about 40 min of both radioactive precursors into nuclear RNA from rat brain. In this nuclear fraction we found at least two different types of RNA that were rapidly labelled. One of them incorporated both [14C]uridine and [3H]methyl groups and seemed to correspond to species of rRNA and their precursors. The other RNA fraction was less methylated or non‐methylated and exhibited sedimentation coefficients distributed along a continuous 8–30 % sucrose density gradient. At least part of the latter type of RNA very probably was mRNA, but much of it must conespond to a different RNA similar to that recently described in HeLa cells by Penman, Vescoand Penman(1968).We also found that labelled 185 and 285 rRNA components began leaving the nucleus for the cytoplasm within 24 to 33 min after the radioactive precursors had been injected, and, in the cytoplasmic fraction, the patterns of incorporation for [14C]uridine and [3H]‐methyl groups were similar for the 18S and 28S rRNA components. We estimate that in this fraction of rat brain the 18S rRNA component was 1·4 times more methylated than the 28S component. We also detected a lower sedimentation coefficient for the non‐ or slightly methylated, species of soluble RNA found in the cytopl

ISSN:0022-3042    

DOI:10.1111/j.1471-4159.1970.tb00505.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

摘要:

Abstract—RNA and DNA were determined in the axoplasm and sheath of squid giant axons. If the RNA was related to axonal length, equivalent amounts of RNA were found in the axoplasm and sheath. However, when it was expressed as a function of dry wt. or residue protein, the concentration of RNA in the sheath was four times greater than in the axoplasm. A ratio of 1·1 was found for sheath RNA/sheath DNA. Less DNA was present in the axoplasm than the amount which could be accurately determined with the methods employ

ISSN:0022-3042    

DOI:10.1111/j.1471-4159.1970.tb00506.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

ISSN:0022-3042    

DOI:10.1111/j.1471-4159.1970.tb00507.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

ISSN:0022-3042    

DOI:10.1111/j.1471-4159.1970.tb00508.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

ISSN:0022-3042    

DOI:10.1111/j.1471-4159.1970.tb00509.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY

ISSN:0022-3042    

DOI:10.1111/j.1471-4159.1970.tb00494.x

出版商:Blackwell Publishing Ltd    

年代:1970

数据来源: WILEY