摘要:

AbstractThe f.p.l.c.Rfor high performance biomolecular purification is now widely used in laboratory and pilot scale separations, both as a means of developing new methods for purification and for analysing the purity of intermediary and final products. The speed of the technique means that it is possible to analyse fractions from a process step and to receive the results within 15–30 min. Two aspects are examined in this paper: the monitoring of protein product formation during fermentation and the analysis of fractions in large scale chromatography. The suitability of the technique in an operating process is discusse

ISSN:0268-2575    

DOI:10.1002/jctb.280360802

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractThis paper describes the automation of fixed‐bed affinity separation techniques for the purification of proteins. The basic requirements of an automated system suitable for carrying out such a separation are detailed together with an introduction to the concept of process optimisation by interactive control. Such systems can continue to optimise separations when operating conditions are variable but require that methods for the on‐line analysis of separation performance are available. Such methods require that the levels of particular proteins in the presence of other proteins can be determined and include continuous measurement of enzyme activity and the use of the new technique of rapid chromatographic analysis. The latter technique, which can also be used more generally in the process control of protein purification, is described and its use illustra

ISSN:0268-2575    

DOI:10.1002/jctb.280360803

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractRapid and reversible protein‐electrode interaction has been achieved through the appropriate design of electrode surfaces and choice of electrolyte conditions. This enabled the direct (unmediated) electrochemistry of redox proteins at a range of electrode materials to be examined. Coupling of the electrode reaction to enzymes, for which the redox proteins act as cofactors, extended the study to systems of biological significance. Electrochemical techniques are finding increasing application in clinical analysis and a new type of amperometric enzyme electrode has been devised which exploits ferrocene derivatives as mediators between enzyme and electrode. The synthesis of ferrocene‐drug conjugates permitted the development of an amperometric immunoelectr

ISSN:0268-2575    

DOI:10.1002/jctb.280360804

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractNear infrared reflectance spectroscopy (NIR) is a rapid (50s) and non‐destructive method of analysis of a wide variety of solid, semi‐solid and liquid samples. Since no sample preparation is required for many sample types, particularly powders, NIR is an ideal technique for process control. The use of NIR to monitor the protein content of flour in order to optimise the milling conditions and the composition of the grist is described. Control of the blending of flours or supplementation with wheat gluten to achieve a composite flour of a given protein content is propo

ISSN:0268-2575    

DOI:10.1002/jctb.280360805

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractProteins produced by recombinant DNA technology will be contaminated with residues from the organism used to express the protein, from its culture medium and from the downstream processing steps. In addition, heterogeneous proteins with incorrect sequence and/or folding may also arise. Urogastrone, a human protein, has been produced by rDNA technology inEscherichia coliand is used to illustrate methods for controlling production and downstream processing steps. Protein chemical, chemical, and biochemical methods for the characterization of the purified drug substance are then described.

ISSN:0268-2575    

DOI:10.1002/jctb.280360806

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

AbstractA faster and accurate infrared method for estimating total aromatics in petroleum samples boiling in the kerosene range (140–250°C) was developed using pure aromatic concentrate from kerosene as reference material. The limitations of a synthetic mixture of pure compounds used as reference are also discussed. The accuracy of results is found to be dependent on the closeness of the average absorptivity at a characteristic wave number (1600 cm−1) of an unknown sample and kerosene used as reference. The results obtained from the proposed method on kerosene fractions of Bombay High are compared, as an illustration, with those obtained by

ISSN:0268-2575    

DOI:10.1002/jctb.280360807

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

摘要:

Abstract2[2‐(benzoxazonyl / quinoxalonyl) ‐ benzfurano (indolo)] ‐ quinolinium ‐1 ‐ ethiodide apocyanine dyes (Va‐fandVIa‐f) were prepared, via interaction of 2‐[2‐(benzoxazonyl‐/quinoxalonyl)‐1,2‐dibromoethenyl]‐quinolinium‐1‐ethiodide (IVa,b) with the appropriate phenols/and or arylamines. The new cyanines were identified by spectral determination. Bactericidal and fungicidal activity of selected cyanines (IIIa,b,Va,VIa,VIh, andVII) were tested against

ISSN:0268-2575    

DOI:10.1002/jctb.280360808

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY

ISSN:0268-2575    

DOI:10.1002/jctb.280360801

出版商:John Wiley&Sons, Ltd.    

年代:1986

数据来源: WILEY