摘要:

Topical steroid treatment is a common therapy for psoriasis. Steroids are known to bind to specific cytoplasmic receptors and to influence gene expression. We investigated the effects of the novel steroid derivative mometasone furoate on the expression of putative target genes in normal human epidermal cells (KC). Gene expression was measured by semiquanti-tative mRNA-PCR. In addition, cytokine receptor characteristics were assessed by ligand binding studies. We found a dose-dependent downregulation of proinflammatory mediators (IL-8, TNFα). Genes involved in growth regulation (HER-2, p53) were also modulated. IL-8 binding to KC was inhibited. We conclude that modulation of the expression of cytokine, cytokine receptor and growth factor genes may contribute to the antipsoriatic action of steroids

ISSN:1660-5527    

DOI:10.1159/000211350

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

The purpose of this study was to determine if the penetration enhancer SEPATM (2-n-nonyl-1,3-dioxolane) would augment the scalp hair growth effects of topical minoxidil in the balding stumptail macaque. A 1-in2 area on the balding scalp of 40 adult female monkeys (four drug-treated and four vehicle-treated groups of 5 monkeys each) was topically treated 5 days/week, q.d. or b.i.d., with approximately 250 μl of minoxidil-SEPATM (2.5% minoxidil, weight/volume in 10% SEPATM, 25% propylene glycol and 65% isopropyl alcohol), Rogaine® topical solution (TS, 2% minoxidil, weight/volume in 20% propylene glycol, 60% ethanol and 20% water) or respective vehicles (without drug) for 16 weeks via paintbrush application. Scalp hair was collected by shaving and vacuuming the dosed area at baseline and at 4-week intervals. The shaved hair was filtered, weighed and recorded as the change from baseline. The q.d. and b.i.d. minoxidil-SEPATM groups displayed a significant increase in hair weight compared to their respective vehicles at week 4 whereas q.d. and b.i.d. Rogaine® TS groups were not active until week 8 and 12, respectively. Both minoxidil-SEPATM treatments produced significantly greater cumulative hair weight over the entire 16-week study compared to either of the Rogaine® TS treatments. Comparable increases in cumulative hair weight were evident between q.d. and b.i.d. minoxidil-SEPATM groups and between q.d. and b.i.d. Rogaine® TS groups. Twenty-four-hour urine samples were collected at steady state to assess urinary excretion of total minoxidil via high performance liquid chromatography analysis in each of the four drug-treated groups. Urinary levels of total minoxidil tended to be greater in minoxidil-SEPATM groups. However, due to high intragroup variation, only the b.i.d. minoxidil-SEPATM group showed a significant 2.8-fold elevation compared to the b.i.d. Rogaine® TS group. These data suggest that the penetration enhancer SEPATM influences the topical delivery of minoxidil resulting in earlier and greater stimulation of scalp hair growth in the balding stumptail macaque.

ISSN:1660-5527    

DOI:10.1159/000211351

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

The main objective of our study was to investigate skin function and structure in human volunteers after electrical current application in order to demonstrate the safety of iontophoresis in vivo. The direct current was applied for 30 min at a density of 0.1 and 0.2 mA/cm2. Iontophoresis increased cutaneous blood flow as measured by laser Doppler flowmetry (LDF). This increase was reversible within 1 h and was more pronounced at higher current density. Measurements of transepidermal water loss (TEWL) and LDF were the same at the cathode and the anode sites. Except for 5 min at 0.2 mA/cm2 density, TEWL values were not enhanced as compared to control values (no iontophoresis). Attenuated total reflectance-Fourier transform infrared spectroscopy of the stratum corneum structure showed a transient increase (30 min) in hydra-tion after electrode application for 30 min. No alteration in lipid structure could be shown. These results augur the safety of drug delivery by iontophoresis.

ISSN:1660-5527    

DOI:10.1159/000211352

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Skin sites on 8 test subjects were treated with moisturizers, and different electrical measuring methods were compared regarding their quality in the assessment of the induced changes in the stratum corneum hydration level. Low frequency susceptance measurements were found preferable to high frequency admittance measurements, and the advantages of monopolar measurements with the three-electrode system are described.

ISSN:1660-5527    

DOI:10.1159/000211353

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

Differences in the skin surface lipid content (SSL) and the dynamic friction coefficient (μ) were investigated with respect to age, sex, and anatomical region in 29 volunteers. The group consisted of (a) 7 young adult females (24.9 ± 1.1 years old, mean ± SD), (b) 7 old females (75.3 ± 2.4 years old), (c) 7 young adult males (28.7 ± 0.5 years old), and (d) 8 old males (73.8 ± 1.9 years old). Measurements were obtained on 11 anatomical regions, namely, the forehead, upper arm, volar and dorsal forearm, postauricular, palm, abdomen, upper and lower back, thigh, and ankle. Skin surface lipid content data were compared with μ measurements to determine the relative contribution of the former to frictional properties of skin, μ and SSL were not statistically different between age groups on all regions except for the ankle, where lipid content was lower in the elderly. Similarly, μ did not vary between sex groups. Skin surface lipid content was statistically lower on the forehead, dorsal forearm and postauricular area in females. Both parameters, however, showed considerable regional variability. A significant linear correlation was established between μ and SSL combining all regions from all volunteers. When μ was plotted against SSL among individual anatomic sites, only the forehead and postauricular area showed significant linear correlations between the two parameters. These data suggest that surface lipid content plays a limited role in frictional properties of skin.

ISSN:1660-5527    

DOI:10.1159/000211354

出版商:S. Karger AG    

年代:1995

数据来源: Karger

摘要:

The roughness parameters known from surface engineering often used to describe the microtopography of the surface of the skin do not, by definition, take into account the two-dimensional relationships of the stratum corneum. An approximate description of these relationships can be obtained by scanning several radial profile sections. A more detailed analysis of the characteristic surface relief of the skin can also be based on the texture parameters used to characterize many natural structures. These types of parameters can be extracted from the gray level images of an impression of the surface of the skin – a replica – that has been magnified under a microscope, photographed with a CCD camera and subsequently subjected to image analysis by a digital compu

ISSN:1660-5527    

DOI:10.1159/000211355

出版商:S. Karger AG    

年代:1995

数据来源: Karger

ISSN:1660-5527    

DOI:10.1159/000211356

出版商:S. Karger AG    

年代:1995

数据来源: Karger

ISSN:1660-5527    

DOI:10.1159/000211357

出版商:S. Karger AG    

年代:1995

数据来源: Karger