摘要:

Source and description:An anonymous clone containing a (TG)15microsatellite was isolated from a genomic library of an N'dama(Bos taurus)bull (designated ND7). Primers for the polymerase chain reaction (PCR) were designed to amplify the region containing this microsatellite. The size of the PCR product was shown to be polymorphic by denaturing polyacrylamide gel electrophoresis. This microsatellite was designated ILSTS001.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00236.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00237.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00238.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00239.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00240.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00241.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

The cells of 60 randomly selected Hannoveranian warm‐blooded horses were subjected to one‐dimensional isoelectric focusing and immunoblotting with a cross‐reacting monoclonal antibody (Bo 1) recognizing bovine class I antigens. The banding patterns were correlated with the serologically defined specificities of the ELA‐A locus. ELA‐A2 was correlated with four bands, while ELA‐A5, ELA‐W18, ELA‐A6, ELA‐A14 and ELA‐A9 were correlated with a single band each. The complexity of the pattern and additional polymorphic bands which could not be correlated to any of the known ELA specificities may indicate biochemical variants of established serological specificities or still‐undetected allelic products of more than one class I locus expressed at the surface of horse cells. Here we present the first description of the biochemical complex polymorphism of equine MHC class I molecules using one‐dimensional isoelectric focusing. Direct comparison of biochemical and serological polymorphisms revealed potentials and limitations of both techniques which supplement each other. Their combination will improve and enhance the definition of expressed products of different loci in

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00242.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

T‐cell recognition of bovine MHC (BoLA) class II antigens was investigated in relation to BoLA class II polymorphisms defined by one‐dimensional isoelectric focusing (1D‐IEF). One‐way mixed lymphocyte reactions (MLRs), and allospecific cell lines and clones were used. In general, T‐cell responses correlated with the 1D‐IEF defined haplotypes (EDF types). However, with MLRs some responses appeared to be associated with BoLA class I differences. All combinations of responder‐stimulator pairs produced alloreactive T‐cell responses both in MLR and in generation of allolines/clones. Thus allospecific lines and clones were generated to all EDF types tested. Splits in the IEF typing were observed with EDF6 and EDF3, indicating that distinct BoLA class II haplotypes are not necessarily distinguished by 1D‐IEF alone. Furthermore, the patterns of reactivity with EDF3 expressing cells were complex with the T‐cell specificities splitting EDF3 into several distinct types. Also, in some cases it was clear that more than one T‐cell specifieity per EDF type was detectable. Thus, allospecific lines and clones provide complementary and additional information to the 1D‐IEF typing for polymorphism of the BoLA class II complex. This extra information is particularly important in terms of the functional significance of the BoLA complex for antigen presentation and immun

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1992.tb00243.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY