摘要:

SummaryA small‐insert bovine genomic library was constructed in pBluescript II SK(+) and enriched for microsatellites by selective rescue of single‐stranded pBluescript DNA carrying (CA)n/(TG)ntandem repeats. Approximately 50% of the clones in the enriched library contained (CA)nrepeats or CA‐rich sequences. Sequencing of clones selected for (CA)nrepeats resulted in the identification and characterization of 45 (CA)npolymorphic microsatellites. Genotyping in 9 large paternal half‐sib families indicated that 40 of these microsatellite markers exhibit autosomal Mendelian inheritance. The numbers of alleles range from 2 to 18, with an average of 6‐3 per locus. The polymorphic microsatellite markers we have identified and characterized will contribute to the construction of a high‐resolution linkage map of bo

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb01175.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryAn approach has been developed for the screening of allelic variation at minisatellite DNA loci that substantially reduces the time and hazards involved. Primers were designed for a minisatellite region isolated from a gadoid fish species[Merlangius merlangusL.), enabling amplification by polymerase chain reaction, so that differences in the number of minisatellite repeat units (allelic variability) were detectable by ethidium bromide fluorescence (over UV light) following separation by agarose gel electrophoresis. This amplifiable minisatellite variable number tandem repeat region, the first non‐primate marker of its kind, can be used successfully with DNA extracted by a rapid Chelex® protocol. From a sample of 97 individuals, 24 alleles were resolved (750–2200 kb) and heterozygosity was estimated at

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb01176.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryA protocol is presented for direct polymerase chain reaction (PCR) amplification of DNA from chicken nucleated red blood cells. Chicken blood in EDTA was found to have a strong inhibitory effect on the PCR. Consequently, PCR using this protocol should be performed only on a narrow range of blood volumes, from 0125 to 025 |ll.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb01177.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryGene and genotype frequencies ofBoLA‐DRB3were studied in seven herds of Argentine Creole cattle. Twenty‐one out of thirty previously identified alleles were detected in this breed. TheFstatistics showed high degree of variability among the studied subpopulations suggesting that subdivision and genetic drift, rather than inbreeding, have been the major factors acting on the observed interpopulation variability. The observed high degree of genetic variation in Argentine Creole cattle could be crucial for the long‐term survival of this population. Maintenance of such polymorphism, as a genetic resource, could be an important issue that will demand attention in future breeding programmes in species under high selective pres

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb01178.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb01179.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb01181.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb01183.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb01184.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb01186.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb01188.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY