ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1995.tb03249.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryThe loci encoding the porcine intestinal receptors forEscherichia coliK88aband K88ac(K88abR and K88acR) were firmly assigned to chromosome 13 by linkage analysis using a three‐generation pedigree. The linear order of these loci and seven other markers on chromosome 13 was determined by multipoint analyses. The K88abR and K88acR loci were tightly linkedwith the K88abR locus localized 7·4 cM (sex average) proximal to the transferrin locus. The results, together with previous reports from two other groups, provide an unequivocal assignment of the K88 receptor loci to chromosome 13, and reject a previous assignment to chromosome 4. Pigs possessing the receptor had a slightly higher specific IgG response to the K88 antigen after an intramuscular immunization with anE. colivacci

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1995.tb03250.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryA small number of west AfricanBos tauruscattle breeds, including the N'Dama, constitute a valuable genetic resource by virtue of their ability to remain productive under trypanosomiasis challenge. However, introgression ofBos indicusgenes into the trypanotolerant breeds, particularly by introduction of zebu bulls, is a threat to this resource. This work describes the characterization and cloning of a bovine randomly amplified polymorphic DNA (RAPD) that is generated in polymerase chain reaction (PCR) with the 10 base primer ILO1065 fromBos indicusmale templates, but not fromB. taurusmale templates or female templates of either type. Male‐specific sequences with homology to the RAPD also occur inB. taurusbreeds. This suggests that the polymorphism may be due to base substitution(s) in an ILO1065 priming site, or insertion/deletion events either affecting priming sites or occurring between sites on the cattle Y chromosome. We have shown that cattle, whether ofB. indicus or B. taurusphenotype, which possess a typicallyB. indicusmetaphase Y chromosome on the basis of QFQ banding, have aB. indicusILO1065‐generated genotype. The ILO1065‐primed RAPD can be used in a simple dot blot assay as a probe of RAPD‐PCR products, to provide a convenient, reliable and effective means of detecting introgression of zebu genes inB. tauruscattle popu

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1995.tb03251.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryRestriction fragment length polymorphisms (RFLPs) detected using cDNA probes for conserved genes provide an important set of markers that anchor or link syntenic groups in a range of divergent mammalian species. DNA probes from sheep, cattle, pig, human and mouse were screened against sheep DNA samples and 24 new RFLP markers for sheep were identified. Among the loci tested, 22 had a homologue that has been mapped in humans. An RFLP for fibronectin (FN1) was linked to α‐inhibin (INHA) at a distance of 5cM. The FN1 locus has been assigned to sheep chromosome 2q41–q44 and linkage between FN1 and INHA assigns INHA to the same chromosome in sheep. In addition to the new loci reported here, 28 RFLPs have been published previously by this group and these are collated together with RFLPs published from other laboratories. RFLPs have been reported for 86 loci in sheep. Fifty‐four loci have been mapped to 16 different chrom

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1995.tb03252.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryThe generalized progressive retinal atrophies (gPRAs) form a group of retinal degenerations of pedigree dogs and cats, which have a variety of genetic origins (mostly unknown). We have examined the opsin gene for polymorphisms in several breeds of pedigree dog suffering from distinct forms of gPRA, by methods including single‐strand conformation polymorphism analysis, microsatellite analysis and direct sequencing. The breeds examined included the Tibetan terrier, the miniature schnauzer, the Irish setter, the miniature poodle, the Labrador retriever and the English cocker spaniel, as well as individuals from breeds in which PRA has not been described and of mixed breed. Individuals from each of the named breeds suffering from PRA were compared with clinically normal dogs. Two polymorphisms were found. One, segregating within the Tibetan terrier population, but not seen in other breeds, was a synonymous transition at nucleotide position 780 in exon 3. Inheritance of this polymorphism suggests that opsin is unlikely to contain mutations causative of gPRA in this breed. The other polymorphism occurred between all miniature schnauzers examined and dogs of other breeds. It consisted of a single base insertion in intron 2. No polymorphisms in the opsin sequence were detected in any other breed. DNA sequencing allowed rigorous exclusion of mutations in opsin as a cause of gPRA in miniature poodles, English cocker spaniels or Labrador retriever

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1995.tb03253.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummarySwine‐specific sequence tagged (microsatellite) sites, STS and STMS, were assigned chromosomally by polymerase chain reaction analysis of a somatic cell hybrid panel. This study confirms the localization from genetic mapping of seven anonymous microsatellites and the genesANPEP, ATP2, CGA, DAGK, FSHB, IFNG, IGF1, IL1BandSPP1.New assignment for the geneBNP1to chromosome 6 is reported. The confirmed and the new assignments extend the information of the previously established linkage maps and provide framework loci on which to order additional informative marker

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1995.tb03254.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

SummaryThree electrophoretic variants of erythrocyte malic enzyme (ME) in goats were reported. Inheritance data indicate that they are controlled by codominant alleles. The allele frequencies in four Mediterranean populations are given.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1995.tb03255.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1995.tb03257.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1995.tb03259.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1995.tb03261.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY