摘要:

SummaryAn immunoblotting technique is reported that reveals electrophoretic variants in the β‐chains of class II antigens of the bovine major histocompatibility complex. One monoclonal antibody, mAb VPM57, reacted on immunoblots with an epitope present in approximately half of the haplotypes investigated. This reagent is especially useful in discriminating electrophoretic variants that have similar isoelectric poin

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00100.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummarySeven bovine erythrocyte antigen loci and three serum protein loci were tentatively assigned to chromosomes or synteny groups by linkage analysis to previously assigned microsatellite DNA markers. The erythrocyte antigen locus EAB was mapped to synteny group U27; EAC to chromosome 18, synteny group U9; EAL to chromosome 3, synteny group U6; EAS to chromosome 21, synteny group U4; EAZ to chromosome 10, synteny group U5; EAR' to chromosome 16, synteny group U1; and EAT' to chromosome 19, synteny group U21. The vitamin D binding protein (GC) and albumin (ALB) loci were assigned to chromosome 6, synteny group U15 and post‐transferrin 2 (PTF 2) to chromosome 19, synteny group U2

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00101.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryA full‐length pig cDNA for the POU‐domain protein OCT2 has been isolated and sequenced. The 478 amino acid‐long reading frame in pig OCT2 is 97% identical to human OCTZA, indicating strong conservation of function for this immunoglobulin regulatory protein. To investigate the potential use of this cDNA for mapping and identifying linkage of OCT2 to economic traits, restriction fragment length polymorphism (RFLP) analysis was used to identify a TaqI polymorphism. A population of 60 unrelated animals, as well as multigenerational families, were typed for this RFLP and showed variability in several American and Chinese breeds. The Taq I polymorphism was also detected by a non‐POU‐domain OCT2 probe, demonstrating that this RFLP is located in the OCT2 gene. This result suggests that OCT2 is likely to be a single‐copy gene in swine as seen in ot

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00102.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryVariability in the most extracellular exons of Atlantic salmon MHC‐Sasa class I and class II was studied by polymerase chain reaction (PCR) amplification followed by sequencing. The domains studied were class I α2 and class II β1. The material used was genomic DNA of fish, mainly derived from the major Norwegian breeding pool, supplemented with some material from a minor breeder and a local river strain. The analysis revealed extensive variation, most individuals being heterozygous with at least two varia

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00103.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryAn essential part in the development of informative linkage maps is to include genetic markers that have been anchored by physical mapping. Here a set of 18 porcine cosmid‐derived genetic markers are reported that have been mapped by linkge analysis, and that also have been physically localized by fluorescence in situ hybridization (FISH). Three different strategies were used to establish polymorphic markers from the cosmid clones. Firstly, dinucleotide microsatellite loci were derived by sequencing cosmid subclones containing (CA), repeats. Secondly, variable SINE 3′ poly(A) tracts (SINEVA) were identified by direct SINE‐PCR amplification of cosmid clones. Thirdly, the cosmids were used in Southern blot hybridization to detect restriction fragment length polymorphisms (RFLPs). Compared with the most recent consensus compilation of the porcine gene map, the present assignment of markers to chromosomes Zp, 3, 4, 10, 12q, and 16 represents the first loci mapped to these chromosomes, for which linkage as well as in situ data are now avai

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00104.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryInternationally recognized allo‐antisera in lymphocyte microcytotoxicity assays are thought to detect allelic products of a single highly polymorphic class I locus. A recent report suggested that two bovine lymphocyte antigen (BoLA) class I loci are expressed at the protein level. However, 1D‐IEF analysis of BoLA class I molecules reveals multi‐band patterns which cannot be reconciled with the reported number of loci. The aim of this study was to investigate the origins of the charge diversity of BoLA class I molecules observed using 1D‐IEF.BoLA class I molecules appear to be glycosy‐lated at a single N‐linked position with a complex type carbohydrate moiety which has up to three terminal sialic acid residues. Class I molecules immunoprecipitated from resting bovine PBL are not phosphorylated. Neither modification is responsible for the observed charge heterogeneity.Peptide mapping reveals that different BoLA charge variants have distinct digestion patterns. Furthermore, a number of different polypeptides are associated with each serological specificity. These polypeptides appear to be encoded by different loci which exist in linkage disequilibrium. The number of charge variants with different peptide maps indicates that the BoLA system has a minimum of three class I loci expressed at the pr

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00105.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryTwo alleles, A and B, were previously described at the goat αs2‐casein locus. Isoelectric focusing allowed us to subdivide the former one in two new alleles, called A and C. Although αs2‐casein C cannot actually be distinguished from its A counterpart by starch or polyacrylamide gel electrophoresis, it differs from the previous allele by a single substitution Lys (A)/Ile (C) at position 167, which was confirmed at the nucleotide level. The frequencies of the three αs2‐casein alleles A, B and C were estimated to be 0.85, 0.04 and 0.11 in the French dairy breeds ‘Alpine’

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00106.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryA panel of 36 hamster‐bovine hybrid cell lines was used to assign 15 bovine microsatellites. Locus identification, synteny group and/or chromosome assignment and registration number were as follows: ILSTS001 (U22, Chr07, D7S13), ILSTS002 (U09, Chr18, D18S7), ILSTS004 (U10, Chr01, D1S28), ILSTS005 (U05, Chr10, D10S25). ILSTS006 (U22, Chr07, D7S8), ILSTS008 (U24, Chr14, D14S15), ILSTS010 (U27, DU27S11), ILSTS011 (U24, Chr14, D14S16), ILSTS012 (U16, Chr11, D11S26), ILSTS015 (U07, Chr25, D25S3), ILSTS016 (U04, Chr21, D21S22), ILSTS017 (X, DXS11), ILSTS018 (U15, Chr06, D6S15), ILSTS019 (U07, Chr25, D25S7) and ILSTS020 (U05, Chr10, D10S27). These results contribute to the international effort to improve the bovine genetic ma

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00107.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryOne cosmid containing a microsatellite (INRA144, D9S14) was assigned to bovine synteny group U2 by somatic cell genetics and localized to bovine chromosome 9q25 by fluorescent in situ hybridization. These results permitted the assignment of one more synteny group to a bovine chromosome. There are now 22 out of 31 bovine synteny groups which are related to a chromosome. The mapping data have been entered in the BovMap database, Jouy‐en‐Josas, Fra

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00108.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryWe have identified substitutions in the 31untranslated region of the bovine myoglobin gene, one of which affects an MboII restriction enzyme site resulting in a bi‐allelic restriction fragment length polymorphism. Co‐dominant inheritance of the alleles in three reference families was observed using a polymerase chain reaction—restriction fragment length polymorphism assay. The distribution of the alleles seems characteristic of cattle type—one of the alleles was not detected in purely taurine breeds. Furthermore, we mapped, using the polymerase chain reaction on a bovine–rodent somatic cell hybrid panel, the myoglobin gene to bovine chromosome five. It is therefore syntenic with γ‐interferon and insulin‐like growth factor in which we have not found polymorphism. The myoglobin locus therefore serves as a type one marker on bovine c

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00109.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY