摘要:

SummaryIn pig, the frequency of intersexuality ranges from 0.1 to O.6%, depending on the breed. In a closed pig herd at INRA an intersex condition was observed in 0.75% of ‘females’. The present study describes 11 animals with a 38XX karyotype and the presence of testicular tissue. Phenotypically, all presented with abnormal external or/and internal genitalia. Southern blot analysis with Y‐specific probes (SRY and ZFY) revealed the absence of Y material in all animals tested. By polymerase chain reaction (PCR) amplification, 10 of 11 intersex pigs lacked the SRY gene in gonad DNA. These data are compatible with an autosomally (or pseudoautosomally) determined mechanism. Moreover, analysis of familial cases seemed to indicate that 38XX male pseudohermaprodites and 38XX true hermaphrodites may represent alternative manifestations of the same genetic d

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00362.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryA Charomid ordered‐array library containing a 2–16 Kb size fraction ofMbeoI‐digested canine genomic DNA has been screened with the Jeffreys multilocus probes, 33‐6 and 33‐15, to identify and isolate canine minisatellite sequences. Of the 48 positive clones identified, 7 were found to contain polymorphic mini‐satellites with heterozygosities in the range 20–88%. The majority of the remainder were either monomorphic or dimorphic in the animals tested. Analysis of intrabreed variation in Bedlington Terriers using two polymorphic minisatellites has shown that a significant reduction occurs in the number of alleles seen compared to an agglomerated population sample, correlating with the high level of inbreeding within this breed. Flanking DNA sequence and partial repeat sequence is presented for the most polymorphic minisatellite thus far identified, cCfaMP5. The variable region in this mini‐satellite is similar to human minisatellites which show a distinct purine or pyrimid

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00363.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryPolymerase chain reaction (PCR) primers designed to amplify the octapeptide repeat region of the bovine prion gene were used to test the association of genotypes with bovine spongiform encephalitis (BSE) in 56 BSE‐affected and 177 unaffected animals. Three alleles (A, B, C) were detected as single‐strand conformation polymorphisms (SSCPs) and two alleles (1,2 representing six or five copies of the octapeptide repeat respectively) were detected as amplified double‐strand fragment length polymorphisms (AMFLPs). Observed genotypes of SSCPs and AMFLPs were analysed by x‐square. The SSCP genotypes of nuclear family members of animals with BSE and BSE‐affected animals were different (P<0.001, P0.05). These data suggest that BSE‐affected animals and their relatives are more likely to have the AA SSCP genotype than unrelated animals of the same breed or animals of d

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00364.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryThe pig chromosome complement of six different types of pig‐rodent hybrid cell lines was examined by means of fluorescencein situhybridization with a porcine SINE probe. The cell lines were obtained by fusing pig lymphocytes with cells of the Chinese hamster cell lines wg3h, BK14‐150 and E36, and of the mouse cell lines NSO, PU and LMTK‐. The hybrids were analysed with respect to: (1) the number of pig chromosomes, (2) the type of pig chromosomes, (3) the occurrence of pig‐rodent chromosome trans‐locations, and (4) the presence of pig chromsome fragments. The results show that the number of pig chromosomes varied within and among hybrid cell lines. The pig‐hamster hybrids mainly retained nontelocentric pig chromosomes, whereas the pig‐mouse hybrids also retained telocentric pig chromosomes. Pig‐rodent chromosome translocations were found in all types of hybrids, but the incidence was in general low. Chromosome fragments were abundant in BK14‐150 hybrids, and rare in most other hybrid cell lines. It is concluded that the SINE probe is a useful tool to make a preliminary characterization of the porcine chromosome complement of pig‐rodent somatic cell hybrids. The results of this characterization can be used to select hybrids for further cytogenetic analysis. Furthermore, our data show that different rodent cell lines will have to be used as fusion partners for the production of hybrids when constructing a panel informative for

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00365.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryIn Poll Herefords, it is known that maple syrup urine disease results from a nonsense mutation in codon ‐6 of the gene for the Elα subunit of branched‐chain α‐keto acid dehydrogenase. The disease also occurs in Poll Shorthorns, but its molecular basis in this breed has not yet been determined. Allele‐specific hybridization and allele‐specific amplification, both based on the Poll Hereford mutation, failed to detect the mutant allele in Poll Shorthorn heterozygotes, and detected the normal allele in affected Poll Hereford‐cross‐Poll Shorthorn calves. These results demonstrate between breed molecular heterogeneity for bovine maple syru

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00366.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryData on allele frequencies at six red cell blood group systems and three blood protein polymorphic loci in five goat breeds are reported. Two blood proteins, albumin and carbonic anhy‐drase, were not found to be polymorphic. The B blood group system of goats, like its homologue in cattle and sheep, is highly complex. At least 44 B phenogroups (haplotypes) have been distinguished in this study. Based on the variation in allele frequencies between breeds, genetic distances were calculated. The distances estimated by four different methods were in close agreement with data from the history and geographic origins of the breeds examine

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00367.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryTen cDNA clones derived from chicken spleen cell mRNA have been partially sequenced and the genes which encode the mRNAs have been located within the linkage map of the chicken genome. The sequences of five of these clones show strong homology to known mammalian genes, the remainder show little homology to sequences present in the current databases. Interestingly, one of these clones appears to be the chicken homologue of the mammalian peptide transporter geneTAP2and is located within the major histocompatibility complex. Two other clones are homologous to genes involved in protein synthesis and these are tightly linked in chickens, as in mice. These results suggest that partial sequencing and mapping of clones from selective cDNA libraries may be an efficient way of adding candidate genes to the chicken linkage map and that on a local scale there may be some conservation of grouping of genes between chickens and mammalian species.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00368.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryA well‐established and characterized somatic cell hybrid panel was used to map three polymorphic microsatellites. MicrosatelliteS0072, representing the linkage groupS0007‐S0072, was assigned to porcine chromosome 14. Micro‐satelliteS0009, representing the unassigned linkage groupEAM‐S0009‐S0071, was assigned tentatively to porcine chromosome 11. Finally,S0062was tentatively mapped to chromosome 18.S0062may represent the first marker for porcine chro

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00369.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryCytotoxic monoclonal antibody IVA 44 was generated after the intraperitoneal immunization with peripheral blood mononuclear (PBM) cells and the boost by the intrasplenic inoculation of skin graft. The detected membrane antigen isolated by immunoprecipitation appears to be composed of two subunits characteristic for the MHC class I molecules. The antibody IVA 44 exhibited a different reactivity: it recognized the BoLA A14 (A8) specificity in animals typed in the Fifth BoLA workshop, while it reacted with all A8 positive animals including subtypes A14 and A15 in Czech and Slovak cattle. It is concluded that mAb IVA 44 might detect the broad subtype of A8 covering A14 and certain A15 split(s). The diverse A15 reactivity of this mAb in the workshop and our population could be explained by the different occurrence of A15 splits in both populations.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00370.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryRestriction fragment length polymorphisms (RFLPs) were revealed at the porcine casein loci with the following combinations of restriction endonucleases and porcine cDNA clones: αs1,‐casein (TaqI); αs2‐casein (BamHI); and ß‐casein (Sacl). These RFLPs were shown to be under simple monogenic control by segregation analysis of two‐ and three‐generation families. The CASAS1, CASAS2 and CASB casein loci were also shown to be linked with no recombinant haplotypes observed amongst 77 meioses in Large White and Meishan F1and F2crosses. No recombinants were observed in a further 106 meioses that were informative for linkage between CASAS

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00371.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY