摘要:

Summary.A BoLA‐DQB cDNA clone (BoLA‐DQß‐1) was isolated by screening a bovine lymphoblastoid cDNA library with a HLA‐DQB genomic clone. The DNA and predicted protein sequences were compared to class II sequences from cattle and other species. BoLA‐DQß‐1 has 92.0% similarity to the coding regions of two previously sequenced BoLA‐DQB genomic clones and 69.6% similarity to a BoLA‐DRß pseudogene. However, the first domain encoded by BoLA‐DQß‐1 has 94 amino acids; one more than the predicted size of the products encoded by two previously sequenced bovine DQB genes (BoDQß‐Q1 and BoDQß‐Y1). Comparing all coding regions, BoLA‐DQß‐1 has greater nucleotide similarity to HLA‐DQB sequences than to I‐Aß, HLA‐DRB and 1‐Eß sequences. Like the HLA‐DQB gene product, the cytoplasmic domain of the predicted protein encoded by BoLA‐DQß‐1 is eight amino acids shorter than that of I‐Aß, HLA‐DRB and I‐Eß molecules. Six clone‐specific amino acid substitutions were identified in the ß1 domain of BoLA‐DQß‐1, including an unusual cysteine residue at position 13 which is believed to be positioned on a ß‐strand and face into the antigen recognition site. Southern blot analysis ofPvuII‐digested genomic DNA from a paternal half‐sibling famil (sire, and six dam‐offspring pairs) using BoLA‐DQß‐1 as a probe, revealed five allelicPvuII RFLP patterns, including two patterns not previously described, that cosegregated with serologically‐defined BoLA‐A (class I) alleles. The evolution, polymorphism and function of a transcriptionally active BoLA‐DQB gene can now be readily studied usi

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00698.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.A series of alloimmunizations were carried out between BoLA class I antigen typed bulls, with the aim of generating class II specific reagents. Of the antisera produced, seven demonstrated exclusively B cell reactivity. Another 19 sera reacted with both T and B cells from some animals and with B cells only in other cases. Suitable buffy coat absorptions removed T cell reactivity from some sera and shortened broader reactivities in certain B cell specific sera. Typing of separated T and B cells from related and unrelated animals permitted clustering of the sera into four groups. These groups behave as allelic specificities.The class II nature of the recognized structures was strongly indicated by two further pieces of evidence. The presence or absence of particular B cell antigens correlated with reactivity of cells in one‐way mixed lymphocyte cultures. In addition, a number of the B cell specific sera were characterized by immunoprecipitation of radiolabelled lymphocytes. The precipitated products corresponded in molecular weight to α and ß chains of MHC class II dimers, as has been found in this and other spec

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00699.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.This paper describes the production of alloantisera directed against bovine major histocompatibility complex (MHC) (BoLA) class II antigens in animals whose MHC phenotypes had been defined by one dimensional isoelectric focusing. Animals of closely matched BoLA class I types were selected by serology and subsequently typed for class I and class II by ID‐IEF of immunoprecipitated antigens. Those with similar class I type by both methods, but differing at the class II locus, were chosen for reciprocal immunization. Cross‐immunization was by two skin implantations 6 weeks apart. The resulting antisera showed low titre after the first immunization and elevated titre 3 weeks after the second immunization. The sera reacted strongly with cells expressing specific BoLA class II antigens. The pattern of reactivity correlated well with IEF class II typing on a panel of animals representing all of the class II IEF types present in the Friesian populat

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00700.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.Ten alloantisera defining five major histocompatibility complex (MHC) class II specificities of the bovine lymphocyte antigen (BoLA) complex were produced and characterized. Eight antisera defining four of the specificities were generated by immunizing cattle with class I compatible‐class II incompatible lymphocytes. The alloantiserum defining the fifth class II specificity was produced by skin implant immunization. A pregnancy serum specific for one of the class II specificities was also identified. The class II antigens recognized by these antisera were designated ‘Dx’ antigens to indicate that they are BoLA‐D region antigens encoded by one or more undetermined class II loci. The molecules identified by the alloantisera are heterodimers composed of a 34‐kd α and a 26‐ to 28‐kd ß chain, and are expressed on B‐lymphocytes but not on resting T‐lymphocytes. In family studies the BoLA‐Dx antigens segregated in linkage with the BoLA‐A locus alleles. Most of the BoLA‐A alleles present in the Cornell Holstein herd at a high frequency were found to exist in gametic association with two or more serologically defined class II haplotypes. On the basis of a population study it was determined that three pairs of class I and class II alleles (w10‐Dx4, w31‐Dx5, and c3‐Dx2) were present in the Cornell herd at sign

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00701.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00702.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00703.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00704.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00705.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00706.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00707.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY