摘要:

Summary.A simple and rapid method of 2D agarose gel (pH 5–4)‐horizontal polyacrylamide gel (pH 9.0) electrophoresis was developed for the simultaneous phenotyping of pig plasma α‐protease inhibitors (protease inhibitor‐1 and ‐2; postalbumin‐1A and ‐1B), postalbumin‐2, transferrin, ceruloplasmin and haemopexin. These eight plasma proteins were clearly visible on gels stained with Coomassie Brilliant Blue G250. The 2D patterns and mobilities of several variants of α‐protease inhibitors were described. By using two agarose gels and 10 polyacrylamide gels, 120 samples were easily analysed in a day. Since α‐protease inhibitors show extensive polymorphism and as the gene for postalbumin‐2 is closely linked to the halothane sensitivity locusHal, this method is a useful tool for conducting parentage control and for predictingHalgeno

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1987.tb00760.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Summary.Two bovine populations, a Holstein‐Friesian dairy stock and a synthetic (Baladi X Hereford X Simmental X Charolais) beef stock, were screened for restriction fragment length polymorphisms (RFLPs) at the growth hormone and prolactin genes. Most RFLPs at the growth hormone gene are apparently the consequence of an insertion/deletion event which was localized to a region downstream of the structural gene. The restriction map for the genomic region including the growth hormone gene was extended. TwoHindIII RFLPs at the growth hormone locus, as well as several RFLPs at the prolactin gene, seemed to be the consequence of a series of point mutations. The results are discussed in terms of the possibility that minor genomic variability underlies quantitative genetic variatio

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1987.tb00761.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Summary.The expression of haemoglobin (Hb) has been studied in 260 Norwegian Dairy goats by the Immobiline technique at pH ranges 6.7–7.7, 6.9–7.6 and 6–9‐7.5. The majority of goats exhibited two‐ or four‐band patterns. In two‐band types the average ratio between the anodal and cathodal band was 74:26. PAGE with 8m urea distinguished three phenotypes for the beta chains, proving that the Hb variation described is in the beta chain. Segregation data in 106 complete sire‐dam‐offspring families agreed with the existence of four β globin alleles – A2, A4, A6and A8. Twenty‐seven animals had reversed ratios (R) of Hb bands. In two‐band phenotypes the average ratio was 36:64. In 15 complete families where one of the parents had reversed ratio, eight offspring received the R type, indicating a simple genetic control. After urea PAGE the R animals all showed the same alpha chain phenotype which differed from that of goats having common ratios of bands. An additional polymorphism appeared in nine animals as three‐ and five‐band patterns which is assumed to be the result of heterozygosity forIIα and forIIα a

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1987.tb00762.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Summary.A cDNA library for porcine skeletal muscle was established in the vector pBR322. The library was screened with an oligonucleotide probe coding for a hexapeptide from glucosephosphate isomerase (Gpi). A positive clone with an insert of about 450 bp and restriction sites forPstI,BamHI andPvuII was isolated. A 362‐bpPstI fragment was sequenced and shown to contain the codons for the hexapeptide as well as the remaining 29 amino acids of this Gpi peptide. ThePstI fragment was used to probe pig genomic DNA. The restriction enzymesPvuII andSacI detected a set of polymorphisms with five bands, behaving as a set of insertion/deletion polymorphism

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1987.tb00763.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Summary.DNA samples obtained from sheep homozygous forHbA, forHbBand heterozygous were subjected to Southern blot analysis using a goat βFand a rabbit β1‐globin gene as probes. Sheep homozygous forHbAshow a different restriction pattern from that of sheep homozygous forHbBwith each of the used endonu‐cleases. The DNAs from moufflon and sheep homozygous forHbAshow indistinguishable restriction patterns with some endonucleases. By means of double digestions it has been possible to construct restriction maps of the βBand βcgenes and to confirm the absence of the βcgene in H

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1987.tb00764.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Summary.Ten genetic markers were studied in seven Spanish native cattle breeds, using a total of 725 animals. Of the ten, two were found to be monomorphic in all seven breeds. The genetic relationships of the seven breeds are estimated by three different genetic‐statistical methods (genetic distances, main coordinate analysis and cluster analysis), which indicate three clearly distinct groups of populations: one where the Cárdena Andaluza and Alistana Sanabresa are very closely related, one comprising Sayaguesa, Morucha, Asturiana de los Valles and Asturiana de la Montaña cattle, and a third, genetically distant from the other two, comprising only Blanca Cacereña. The dendrogram drawn from the genetic distances matrix would seem to imply that the seven breeds are descended from different ances

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1987.tb00765.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Summary.Five erythrocyte proteins (Adenosine deaminase, Ada; 6‐Phosphogluco‐nate dehydrogenase, Pgd; Esterase 1, Es‐1; Esterase 3, Es‐3; NADH‐Diaphorase 2, Dia‐2) and a serum β‐globulin protein (Esterase 7, Est‐7) were studied in rabbits using starch gel electrophoresis. Samples were obtained from 317 Spanish Common individuals (38 families, 317 individuals).For the analyses of linkage, Morton's sequential probability ratio test was applied. Clear evidence for linkage between Es‐1 and Est‐7 (θ≅ 0.2) was obtained, and no evidence of linkage was obtained for the remaining pairwise combi

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1987.tb00766.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Summary.Altogether, 292 goat alloantisera were screened for antilymphocyte reactivity in a two‐step dye exclusion microcytotoxicity test. Fifteen different lymphocyte antigen specificities were characterized by cluster analysis and absorption studies. The specificities were designated N1‐N15 (N for Norwegian). Lymphocytes from 247 Norwegian dairy goats were tested. Each animal displayed from none to four of the characterized specificities. Lysostrip testing and family studies indicated that the specificities N1‐N14 were coded for by multiple alleles belonging to at least two closely linked loci. It is suggested that these loci are part of the caprine major histocompatibility complex. Family studies gave strong evidence that the specificity N15 was not coded for by genes located in the same region as the other 14 specificities. Absorption studies showed that this specificity was located on both lymphocytes and erythro

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1987.tb00767.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Summary.In German Simmental cattle a new K‐casein variant was detected by isoelectric focusing, which cannot be distinguished from K‐casein A by polyacryla‐mide and starch gel electrophoresis. It was named K‐casein D. The frequencies of K‐casein alleles were K‐Cn A 0.75, K‐Cn B 0.24

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1987.tb00768.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Summary.This paper describes for the first time polymorphism of the erythrocyte diaphorase in goats. Three diaphorase 1 phenotypes were observed in the red cells of goats. Breeding data indicated that polymorphism was controlled by two autosomal codominant alleles,DiaFandDias, the frequencies of which were determined in 14 different Spanish breeds of goat.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1987.tb00769.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY