摘要:

SummaryA report of the first workshop on the genetic map of bovine chromosome 23 (BTA23) is given. Five laboratories contributed data from 29 loci, including a total 11586 informative genotypes. The combined pedigrees represented 1930 potentially informative meioses. Eighteen of the 29 loci were common to two or more data sets and were used to construct a framework linkage map of BTA23. Twelve of the 18 could be ordered on the linkage map with a likelihood ratio of greater than 1000:1. Thus, a low resolution consensus map was constructed with a high level of support for order. The sex‐averaged, female and male maps span 54.5, 52.7 and 55.8 cM, respectively. Sex‐specific differences in recombination frequency were identified for eight pairs of framework loci. Average genetic distance between framework loci on the sex‐averaged map is 5

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb00470.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryStarch gel‐electrophoresis was used to assess genetic differences between two populations ofBarbus motebensisand two other taxonomic related and morphologically similar barb species. Ten of the 29 protein coding loci studied were polymorphic. In spite of the small bio‐metric and meristic differences between the species studied, the extent of genetic differences suggests four distinct species. The evidence in support of a new barb species in the Ohrigstad River further adds to the current conservation importance of the rivers of the eastern Transvaal escarpment as a ‘hotspot’ for endemic fish

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb00471.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryA cDNA clone encoding the complete coding sequence for porcine alpha‐1‐antitrypsin (or α1‐protease inhibitor,PI) was isolated and its DNA sequence determined. The cDNA is assumed to encode alpha‐1‐antitrypsin on the basis of its sequence similarity to the corresponding cDNAs for human, baboon, rat, mouse, sheep and cow. The porcine cDNA clone was used in conjunction withBamHI,KpnI, MspI, SacI, TuqIandXbaIto develop restriction fragment length polymorphism‐based genetic markers for linkage mapping in pigs. The cDNA has also been used to map the porcinePIlocus to chromosome 7q2.4‐>q2.6 by radioactivein situhybridization. Thus, thePIlocus has been added to the developing physical and genetic maps of the

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb00472.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryAlthough the complete bovine mitochondrial DNA molecule has been previously sequencedand sequence comparisons of the mitochondrial displacement loop have been performed, detailed sequence information is limited on coding regions of mitochondrial DNA within and among breeds ofBos taurusandBos indicus.This study analysed polymorphism of the mitochondrial DNA transfer RNA genes for trypto‐phan, alanine, asparagine, cysteine, tyrosine and the origin of light strand replication among Ayrshire, Canadian, Belgium Blue, Brown Swiss, Hereford, Jersey, Limousine, Piedmon‐taise, Red Angus, Simmental (Bos taurus) and a Nellore (Bos indicus). Nucleotide sequence analysis of a 420‐bp fragment of mitochondrial DNA comprising the five transfer RNA genes showed 100% homology among single individuals of theBos taurusbreeds. The Nellore breed showed guanine to adenine substitutions in the DHU arm of asparagine tRNA and in the origin of light‐strand replication. This equates to a 0.5% sequence difference between the Nellore andBos taurusbreeds and may reflect an independent evolutionary origin of the

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb00473.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryThe distribution of PRE‐1 sequence (a swine SINE) among the animal species related toSus scrofa, i.e.Phacochoerus aethiopicusandTayassu tajacu, was examined by dot‐blot analysis using PRE‐1 sequences as a probe. This revealed thatPhacochoerus aethiopicusandTayassu tajacucontained PRE‐1 sequences, amounts of which in their genomes are almost the same as that in the swine genome, indicating that these species separated after PRE‐1 sequences proliferated to diversify in the genome. In order to estimate the time when the PRE‐1 started to diversify in the swine genome, PRE‐1 sequences were extracted from GenBank DNA database by homology analysis using the PRE‐1 consensus sequence as a probe. The 22 PRE‐1 sequences obtained were aligned and their phylogenetic relation was calculated by the neighbour‐joining method. The result of the calculation combined with the mutation rate of the pseudogenes (r = 4.6 times 10‐9indicated that the PRE‐1 sequence diversified at least 43.2 million years ago. Taken together, the period of time since the separation of the three species,Sus scrofa, Phacochoerus aethiopicusandTayassu tajacu, is currently estimated to be less

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb00474.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryThe ovine map is not yet well‐developed, which represents a problem when looking for markers of a region of interest in sheep. A means of circumventing this is to use comparative mapping. In this study primers were determined using consensus sequences for the epidermal growth factor gene of humans, rats and mice, and an ovine epidermal growth factor gene fragment was amplified by polymerase chain reaction (PCR). A new set of specific ovine primers was chosen to study the polymorphism of this DNA fragment by denaturing gradient gel electrophoresis. Eighty‐four individuals belonging to seven sheep breeds were studied with this technique and four alleles were detected. The heterozygosity rate was 0.57. Family analysis showed mendelian inheritance of the alleles. Usually, genetic analysis of type‐I loci used in the comparative mapping is based on the detection of restriction fragment length polymorphisms in sheep DNA using cDNA probes from other species. Our work shows that another method, based on PCR and denaturing gradient gel electrophoresis techniques, can be efficiently

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb00475.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryAn alloimmune reagent has been produced that distinguishes the equine factor Df in the D phenogroup, cfmqr, from that occurring in cefmqr and dfklr. Using this reagent it has been possible to correctly genotype Dc, d, f, k, l, m, q and r positive cells without recourse to family data.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb00476.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryA new k casein variant (k‐CN G) with a frequency of 0.003 was demonstrated in Pinzgauer cattle from Austria and Bavaria, Germany by iso‐electric focusing in polyacrylamide gels and by alkaline polyacrylamide gel electrophoresis. k‐CN G was not present in milk samples of Limpurger, another endangered

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb00477.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryA two‐point linkage analysis was performed between blood group (14), allotype (8), polymorphic protein (11), DNA type I (2), and microsatellite (2) loci in Wild Boar x Pietrain and Meishan x Pietrain three‐generation families. The following new pairwise linkages were detected:LPR‐EAN(Zmax= 60.68, Ø= 0.055),EAD‐GH1(Zmax= 17.43, Ø= 0.246),EAO—P3(Zmax= 15.81, Ø= 0.239), andP3—S0003(Zmax= 5.43, Ø= 0.312). This study and published mapping data enabled the localization ofLPR(LPR allotype) to chromosome 9,EAD(erythrocyte antigen D) to chromosome 12, andEAO(erythrocyte antigen O) andP3(P3 allotype) to the q arm of chromosome 6 with gene orderSOOO3—P3—EAO, EAObe

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb00478.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

SummaryA 323‐bp DNA fragment (U15557) was isolated, cloned, and sequenced after polymerase chain reaction (PCR) amplification from Monodelphis domestica genomic DNA. AHindIII restriction fragment length polymorphism was identified in this species using the U15557 PCR. fragment as a hybridization probe. DNA samples exhibited either a 6.4kb band, a 7.2 kb band, or both bands simultaneously. Behaviour of these two variants in family studies was consistent with codominant autosomal inheritance. Linkage between this marker and the loci encoding protease inhibitor (PI) and adenylate kinase 1 (AK1) was found in M. domestic

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1996.tb00479.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY