摘要:

Summary.Utilizing a ‘sandwich’ ELISA assay we have been able to demonstrate that mAb W6/32, B1G6 and IL‐A19 are reactive with three different monomorphic determinants on bovine class I major histocompatibility complex (MHC) molecules. Sequential immunoprecipitations performed with the mAb revealed that class I molecules on PBM comprise a single population with respect to reactivity with the mAb in that the β2m‐associated proteins bear all three epitopes. By contrast, TCGF‐driven lymphoblasts and cells transformed byTheileria parva(Tp) additionally express molecules of Mr 45000 bound to β2m which are recognized by mAb B1G6 and IL‐A19 but not by W6/32. These two subclasses of molecules were further distinguished on the basis that, when tunicamycin was added to cultures in the preparation of cells for analysis, mAb W6/32 precipitated class I heavy chains of Mr 39000 while the extra molecules detected only by mAb B1G6 and IL‐A19 were of Mr 37000 and 39000. On thymocytes, the mAb W6/32‐non‐reactive class I molecules are present in low amounts and are expressed by cells in the medulla area, unlike BoT1 (analogous to human CD1) molecules which are expressed by the cortical cells. Our studies also revealed that the supposed β2m‐specific mAb B1G6 does not recognize the β2m‐associated molecules (BoT1) precipitated by mAb TH97A and thus the specificity of mAb B1G6 in cattle is for an epitope on bovine β2m which is strongly influenced by the nature of the heavy chain with w

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00865.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.Genomic DNAs from animals representing six breeds of cattle (Angus, Brahman, Hereford, Holstein, Jersey and Texas Longhorn) were screened with cloned gene probes in a search for restriction fragment length polymorphisms (RFLPs). Eleven RFLPs were identified using seven different probes: growth hormone, prolactin, osteonectin, α A‐crystallin, γ crystallin, fibronectin and 21‐steroid hydroxylase. The frequencies of the alleles identified by each probe were calculated and compared in a limited sampling of the six bovine breeds. These polymorphisms greatly enhance the pool of immunogenetic, biochemical and molecular markers available in cattle for linkage analysis, testing of parentage, and distinction of b

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00866.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.Class II genes of the bovine major histocompatibility complex (MHC) have been cloned from a genomic library. The library was constructed in the bacteriophage Λ vector EMBL3 and comprises approximately 10 times the equivalent of the haploid genome. Half the library was screened with the human DQA, DQB, DRA and DRB cDNA probes. Of the 100 positively hybridizing phage clones, 37 were eventually fully characterized and mapped by means of Southern blot analysis. The exons encoding the first, second and transmembrane domain of all different A and B genes were subcloned and mapped in more detail. These analyses showed that these 37 clones were derived from five different A and 10 different B genes. The hybridization studies indicate that we have cloned and mapped two DQA genes, one DRA gene, two other A genes, four DQB genes, three DRB genes and three other B genes. Since the library was made from a heterozygous animal, this would suggest that there are at least one DQA, one DRA one other undefined A, two DQB, two DRB and one or two other undefined B genes in the haploid genome of Holstein Friesian cattle

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00867.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.The Namaqua is an indigenous fat‐tailed African breed of sheep which has remained relatively isolated and which at one time dwindled to near extinction. Frequency data are given for blood group antigens, red cell glutathione and potassium types, for electrophoretic variants of red cell haemoglobin, ’X’ protein, nucleoside phosphorylase, NADH‐diaphorase, lysine and carbonic anhydrase and of plasma esterase, transferrin and albumin. Of particular interest was the occurrence of the i blood group, a bimodal distribution in red cell glutathione concentrations and red cell potassium concentrations of around 57 mmo1/1 cells, i.e. neither typically LK nor

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00868.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.Phosvitin polymorphism in egg yolk of the Japanese quail was found by horizontal polyacrylamide gradient gel electrophoresis. Six phenotypes of yolk phosvitin designated A, B, C, AB, AC, and BC were observed in a population of 281 birds. Analysis of family data revealed that the phenotypic variation of quail yolk phosvitins was controlled by an autosomalPvlocus with three codominant alleles, Pva, Pvband Pvc. The gene frequencies of Pva, Pvband Pvcwere 0.064, 0.824 and 0.112, respectively.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00869.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.Described is an alternative procedure for the phenotyping of pig α1B‐glycoprotein (PO2) and haemopexin. The procedure is based on the separation of serum samples by horizontal polyacrylamide gel electrophoresis, passive blotting onto a nitrocellulose (NC) sheet, and immunochemical detection using a mixture of a primary antibody (rabbit anti‐pig α1B or anti‐pig haemopexin) and a peroxidase‐labelled secondary antibody. Several NC copies can be obtained from a single gel and these can be developed with different monospecific

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00870.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.The studies on α‐protease inhibitors systems, controlled by four tightly linked loci, were performed within 159 families of Norwegian Landrace (NL) and 40 families of Czech Landrace (CL) pigs. Significant differences in allele and haplotype frequencies between the two breeds were shown. TheSE‐FandSSsShaplotypes (Pil, PolA, PolB, Pi2loci) appeared to be the most frequent haplotypes in NL and CL breeds respectively. This system of blood plasma proteins can be very useful for studying the relationship between br

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00871.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.Evidence for genetic linkage between the loci for transferrin (Tf) and ceruloplasmin (Cp) in pigs was presented. The results were based on a study of a single sire family comprising 35 informative offspring. No recombinants were observed. The recombination frequency was estimated to be in the range of 0 to 8%. This indicated that the recombination frequency betweenTfandCploci in pigs may be much lower than that reported previously between these two loci in cattle and in human.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00872.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.Three new full‐length cDNAs coding for porcine somatotropin (PST) have been cloned. The sequence data indicate a high degree of polymorphism in the PST sequence. All six known PST sequences are differen

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00873.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Summary.A method for K‐casein genotyping in bulls has been developed. By analysis of DNA polymorphisms we are able to discriminate between the K‐casein variant A and B in the bulls. This method will be an efficient tool in selection for the most desirable K‐casein va

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1989.tb00874.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY