摘要:

Summary.The polymerase chain reaction makes it possible to analyse DNA sequences in a single cell and has led to a new approach for constructing genetic maps. We describe a procedure called ‘sperm typing’ which can accurately classify individual meiotic products as recombinant or non‐recombinant. This permits the linkage relationships among DNA polymorphisms to be determined without pedigree ana

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00652.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.Four genes having homologous loci on the short arm of human chromosome 8 have been mapped to two different bovine syntenic groups. The gene coding for the tissue‐type plasminogen activator mapped with GSR, a human chromosome 8 marker, of syntenic group U14 while lipoprotein lipase and the medium and light neurofilament polypeptide genes were shown to be syntenic with the human chromosome 9 marker GGTB2 of syntenic group U1

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00653.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.Restriction fragment length polymorphisms (RFLPs) have been identified in the bovine MHC class II region using five hybridization probes constructed from two bovine genomic clones. Four probes were constructed from a bovine DRß‐like gene,BoLA‐DRB2.These included a probe containing the complete ß1exon (R2‐ß1), a probe containing the last 129 base pairs of the ß2exon (R2‐ß2), a probe containing intron immediately 5′ of the ß2exon (R2‐5′ß2), and a probe containing the complete transmembrane exon (R2‐TM). A fifth probe was constructed from a novel bovine ß‐chain gene,BoLA‐DIB,and contained the entire TM exon (II‐TM). R2‐ß1defined very little polymorphism. R2‐ß2hybridized to several fragments but one or two fragments hybridized much stronger on all Southern blots and it was presumed these corresponded to BoLA‐DRB2 fragments. By using R2‐5′ß2as a probe, these BoLA‐DRB2 fragments were confirmed: 6.4 and 2.7‐kbEcoRl alleles, 1.7‐and 1.5‐kbPvuII alleles, 5.9‐, 5.4‐, 3.7‐and 1.9‐kbTaqI alleles, and a non‐polymorphic 22.5‐kbBamHI fragment. 11‐TM identified three alleles withTaqI. To investigate the linkage between the RFLP alleles, 166 offspring of five sires were tested. Complete linkage was found for all RFLPs identified with the BoLA‐DRB2 probes. However, the RFLP patterns of 13 ca

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00654.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.Oligonucleotide probes specific for simple tandem repeat sequences produce individual specific DNA fingerpints in man and all animal species tested so far. Here 11 different synthetic probes were hybridized to bovine genomic DNAs which had been digested with the restriction endonucleasesHinfl, AluI andHaeIII. Two of these probes gave DNA fingerprint patterns which were analysed for three German breeds. Different parameters were calculated, such as the average number of bands per individual or the probability of finding identical fingerprints in twounrelated individuals. The number of polymorphic bands varies from 11 to 23 in the different breeds and the probability of finding the same banding pattern in two unrelated individuals ranges from 1.5.10‐7. to 2.4.10‐7. Hence this DNA fingerprinting procedure allows precise identification of individuals. It is also a useful additional method for paternity testing in cat

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00655.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.One‐dimensional isoelectric focusing followed by immunoblotting and development of the immunoblots with the monoclonal antibody HC‐10, raised against denatured HLA class I heavy chains, was used to demonstrate biochemical variation in cattle MHC (BoLA) class I molecules. The bands obtained correlated well with BoLA‐A specificities. Two or three bands were identified for the specificities w7, w8, w16, w18, w21, cph43 and cph49, whereas no bands were observed for the specificity w2. Two serologically indistinguishable subtypes of specificity w18 were ident

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00656.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.Seven fusions of mouse myeloma cells with spleen cells from mice immunized with bovine red cells yielded 61 clones producing discriminant antibodies out of a total of 651 secreting clones. Although antigenic factors of all known bovine blood group systems were present on the donors' cells, the antibodies identified reacted with antigenic factors from only five systems, A, B, F, S and Z. The antibody specificities produced by more than two clones were anti‐A1or ‐A2(21 clones), ‐S (9),‐Z (6), ‐G‘ (3) and ‐V1(3). The absence of clones secreting antibodies to antigens of the other systems, especially the complex C system, remains unexplained. The properties of the antibodies reacting with antigens of the S system (anti‐SU“, anti‐SUU’) and of the B system (O‐like antibodies) are in accordance with previous interpretations of polyclonal sera and with present knowledge of the gene

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00657.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.DNA extracted from 25 hamster‐sheep hybrid cell lines was subjected, after Southern blotting, to hybridization with CASB, CASK, LALBA, IGF‐1 and AMH cDNA probes. CASB and CASK segregated together and IGF‐1 and LALBA were found syntenic with the LDHB‐PEPB‐TPI‐GAPD‐SHMT‐KRTB group. No other synteny was observed with any of the previously described groups using the same hybrid cell panel.Gene nomenclature:ACO 1: aconitase 1 (soluble); ADA: adenosine deaminase; AMH: antiMüllerian hormone; ARA 1: murine sarcoma 3611 viral (v‐raf) oncogene homologue 1; CASB: ß‐casein; CASK: K‐casein; ENO 1: enolase 1 (alpha); GAPD: glucose‐6‐phosphate dehydrogenase; GALA (or GLA): galactosidase (alpha); GAPD; glyceraldehyde‐3‐phosphate dehydrogenase; GPI: glucose phosphate isomerase; GSR: glutathione reductase; HBG: haemoglobin gamma; HPRT: hypoxanthine phosphoribosyl transferase; IDH 1: isocitrate dehydrogenase 1 (soluble); IGF‐1: insulin growth factor 1; ITPA: inosine triphosphatase; KRTA: keratin (acid); KRTB: keratin (basic); LALBA: aT‐lactalbumin; LDHA: lactate dehydrogenase A; LDHB: lactate dehydrogenase B; MDH 2: malate dehydrogenase NAD (soluble); ME 1: malic enzyme (soluble); MPI: mannose phosphate isomerase; NP: nucleoside phosphorylase; OLA: ovine leucocyte antigen: OTC: ornitine carbamoyltransferase; PAIS: phosphoribosyl amino imidazole synthetase; PEPA, PEPB, PEPC: peptidase A, B, C; PGD: phospho gluconate dehydrogenase; PGK: phosphoglycerate kinase; PGM 3: phospho glucomutase 3; PKM 2: pyruvate kinase (muscle); PLP: proteolipid protein; PRGS: phosphoribosyl glycinamide synthetase; RCP: red cone pigment; SHMT: serine hydroxymethyl transferase; SOD 1: superoxide dismutase 1 (soluble); SYN 1: synapsin 1;

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00658.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.New adenosine deaminase variants ADA C and ADA D were found by means of agarose gel electrophoresis in pig erythrocytes. Family data supported the hypothesis that these are controlled by codominant allelesADAcandADAD. TheADAcallele was present in Large White(q=0.076), Landrace(q=0.037). and their crosses with other breeds. TheADADallele was present in Duroc(q=0.067) and its crosses. Allele frequencies for six pig breeds are given.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00659.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.In a search for new fingerprinting probes for use with sheep, repeat sequences derived from five poxviruses, an iridovirus and a baculovirus were screened against DNA from sheep pedigrees. Probes constructed from portions of the parapox viruses, orf virus and papular stomatitis virus and the baculovirus from the alfalfa looper,Autographa californica,nuclear polyhedrosis virus all gave fingerprint patterns. Probes from three other poxviruses and an iridovirus did not give useful banding patterns.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00660.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Summary.By means of a new antiserum, Mm, the Mae phenotype can be shown to be controlled by the Maemallele, the Mef phenotype by either the original Mefor a new Mefmallele, and the Mbe(f) phenotype by the Mbe(f)mallele. The complexity of the porcine M system is now extended to 13 internationally recognized blood group factors controlled by at least 19 alleles.

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1991.tb00661.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY