摘要:

SummaryThis paper reports the detailed characterization of multilocus minisatellite DNA fingerprints in the chicken. Results are presented of DNA fingerprint segregation analyses carried out in three chicken pedigrees, calculating the number of detected loci, testing for Mendelian inheritance, and cosegregation among fingerprint bands. Two pedigrees (families 1 and 2) were analysed using the Jeffreys probes 33.6 and 33.15 only, and one pedigree (family 3) was analysed using 33.6, 33.15. 3′α‐globin HVR and M13 protein III gene repeat. Mean band transmission frequencies in families 1 and 2 were near to the Mendelian expectation of 0.5 and no mutations were observed. Family 3 showed transmission frequencies slightly exceeding 0.5. Linkage among bands was higher than observed in some other avian species, with each allele represented by a mean of 1.48HaeIII fragments. Cosegregation of heterozygous parental fragments representing distinguishable loci followed the expected binomial distribution. The number of minisatellites detectable by the four probes was estimated to be 217. The pattern of cosegregation among those minisatellite loci was tested against that expected for different levels of recombination through the use of a simulation model. We conclude that most minisatellites are unlinked and probably widely dispersed in the chicken ge

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00528.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryThe isolation of chicken minisatellites is reported. Two charomid libraries were constructed from fractions of size‐selected MboI‐digested genomic DNA. A total of 5985 colonies were screened with the multilocus probes 33.6 and 33.15; 137 positives were obtained (2.35%). A total of 55 clones were tested against four unrelated chickens; 30 revealed variable single locus patterns. More detailed characterization was made of 15 probes, which were tested against four families comprising a total of 53 offspring. Of parent/offspring comparisons 51% were informative for segregation. No mutations were detected. Three minisatellites were linked on the same autosome, implying that these loci may occur in clusters, and one was Z chromosome‐linked. Heterozygosity and allelic variability were measured in 67 individuals to several different strains and breeds. Mean heterozygosity ranged from 50 to 84%. Minisatellite loci appear to be highly variable in the chicken and should provide highly informative markers in intraspecific crosses in genome mapping st

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00529.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryPrimer sequences flanking 13 microsatellite loci isolated from the domestic horse (E. caballus) were successfully used to amplify homologous loci in the Przewalski's horse (E. przewalskii). The results demonstrate that the level of polymorphism at all 13 loci in the Przewalski's horse was comparable to that in the domestic horse and the overall exclusion probability in the Przewalski's horse was calculated to be 0.9994. The results suggest that it should be possible to useE. caballus‐derived microsatellite markers to provide parentage verification and additional valuable information to the captive management ofE. przewalskii.The ability to amplify corresponding loci in the remaining five species of the genus was also confirmed, illustrating the general application of markers isolated from the domestic horse to the evaluation of polymorphism in the other six species of the genu

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00530.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryVariation in the nucleotide sequence of the B2 high‐sulfur protein genes has not been reported previously. This paper reports 15 nucleotide substitutions in each of the genes for the B2A and B2C proteins and a length of polymorphism in the B2A gene which translates to the insertion/deletion of one 30‐nucleotide repeat sequence. Evidence is presented for gene conversion occurring within the B2 high‐sulfur multigene family. These DNA polymorphisms may account for some of the microheterogeneity observed in the B2 high‐sulfur proteins and may also be useful genetic markers of the B2 high‐sulfur protein gene loci for future use in analysing wool fibre charac

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00531.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryThe second exons ofOLA‐DQBgenes from 13 merino sheep were sequenced following amplification by the polymerase chain reaction or isolation from a cDNA library. Ten distinct exon 2 sequences, coding for 10 novel amino acid sequences, were characterized in these sheep. The single‐strand conformation polymorphism technique was shown to be capable of discriminating between all sequences. This brings the total number of differentOLA‐DQBexon 2 sequences (nucleotide and amino acid) which have been characterized to 12, and demonstrates that theOLA‐DQBregion is highly polymorphic with 29% of nucleotide and 46% of amino acid sites showing variation. Evidence is presented that theOLA‐DQBsequences belong to at least two lineages ofDQBgenes. Some ovineDQBsequences are more like bovineDQBcounterparts than other ovineDQBsequences suggesting that the artiodactylDQBgene and allele lineages predate the separation of the ovine and bovine species 20 million

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00532.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

SummaryA method for determining the sex of bovine embryos has been established. Primers for a portion of the bovine amelogenin locus (AMX/Y) were used to amplify DNA present in either 0.1 μ1 of blood or biopsies taken from 6–7‐day‐old embryos. The primers amplify a 280 bp band in females and a 280 and 217bp bands in males. The method is rapid, does not require prior purification of DNA and contains an internal control which detects PCR f

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00533.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00534.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00536.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00537.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0268-9146    

DOI:10.1111/j.1365-2052.1994.tb00540.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY