摘要:

AbstractThe production of xylanase in the presence of various polymeric substrates was studied. Among the polymeric substrates, xylan was the best inducer of xylanase byV. albo‐atrum.Other cellulolytic enzymes such as glucanase and β‐glucosidase were synthesized in smaller quantities. The process of degradation indicated that xylanase behaved as typical endo enzyme causing first production of high mol. wt. products. Studies on xylan medium indicated thatV. albo‐atrumproduced at least three enzymes which degrade

ISSN:0233-111X    

DOI:10.1002/jobm.3620310502

出版商:Wiley‐VCH    

年代:1991

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620310504

出版商:Wiley‐VCH    

年代:1991

数据来源: WILEY

摘要:

AbstractIn recombinantEscherichia colistrains the subcellular location of human interferon (IFN)α2 and a hybrid IFNα1/α2 was investigated by immunogold labelling techniques. The gold label was scattered throughout the cytoplasm in cells containing the gene for mature IFNα2 under the control of heterologous staphylokinasesak42D transcription and translation initiation signals. In contrast, in cells containing in addition thesak42D signal peptide coding region in front of the IFN gene, the gold label was found mainly near the cell membrane and in the periplasmic space. Inclusion bodies were identified in cells accumulating IFN in the cytopl

ISSN:0233-111X    

DOI:10.1002/jobm.3620310505

出版商:Wiley‐VCH    

年代:1991

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620310507

出版商:Wiley‐VCH    

年代:1991

数据来源: WILEY

摘要:

AbstractProtein A is a cell wall linked protein ofStaphylococcus aureusthat binds mammalian IgG. Although protein A displays high size heterogeneity among strains, cloning and sequencing of its gene from two strains had not shown a large difference in size. Here we report a third protein A gene sequence that shows a size variation relative to these two, due to deletions on both one IgG binding domain and a cell wall binding domain (region X). By analysis of the three sequences we were able to delineate a hypothetic model for region X domain evolution and discussed the origin of genetic variability within and without strains.

ISSN:0233-111X    

DOI:10.1002/jobm.3620310508

出版商:Wiley‐VCH    

年代:1991

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620310509

出版商:Wiley‐VCH    

年代:1991

数据来源: WILEY

摘要:

AbstractCytochrome P‐450 was shown to be involved in 11α‐, and 11β‐hydroxylation of Substance S in intactC. elegansprotoplasts. The steroid transformation was inhibited by carbon monoxide, the inhibitory effect being dependent on CO concentration. The function of cyt P‐450 in intact protoplasts was confirmed by the estimation of strong absorption at 450 nm in the CO difference spectrum. The presence of antimycin A was necessary to prevent the reduction of the cytochrome oxidase and its interference with the cyt P‐450 in the spectrophotometric analysis.The intracellular content of cyt P‐450 could be increased from 5.25 pm/mg protein to 26.88 pm/mg protein when the steroid inducer was present in the medium at each stage of protoplast preparation and during cyt P‐450 determination.The enriched microsomal fraction obtained from the crude extract of ruptured protoplasts contained the steroid 11α‐hydroxylase system ofC. elegans.The activity of 11β‐hydroxylase could not be detected under the conditions of the experiment. The localization of steroid hydroxylases ofC. elegansin microsomes was confirmed by cyt P‐450 detection in the 9600 × g supernatant. Membranous fractions (pellets 1100 × g and 9600 × g) of the concanavaline A stabilized protoplasts, carrying the marker plasma‐membrane‐bound and mitochondrial ATPases, did not show maximum absorption at 450 nm

ISSN:0233-111X    

DOI:10.1002/jobm.3620310510

出版商:Wiley‐VCH    

年代:1991

数据来源: WILEY

摘要:

AbstractA strain ofAphanocladium albumwas found to reduce carbonyl group at C‐3 in 5α‐ and 5β‐dihydroderivatives of testosterone and androstendione. Stereochemistry of this reduction depends on the geometry of ring junction as well as on the presence of substituents (CH3at C‐4, OAc at C‐17, or OH at C‐19). The simultaneous presence of both CH3at C‐4 and OAc at C‐17 inhibits the reduction totally in both 5α‐ and 5β‐isomer. The substrates containing a carbonyl group at C‐17 we

ISSN:0233-111X    

DOI:10.1002/jobm.3620310511

出版商:Wiley‐VCH    

年代:1991

数据来源: WILEY

摘要:

AbstractAnArmillariella melleaculture was found to transformtrans‐ sobrerol to a very small extent, whereas menthol resisted its action altogether. Acetylation of both alcohols, on the other hand, reappointed their reactivity in the sameA. melleaculture. Thus, trans‐sobrerol monoacetate and menthyl acetate underwent a two‐step transformation involving hydroxylation (preserving the acetate link) to 3‐acetoxy‐6,8‐p‐methen‐diol and 8‐hydroxymenthyl acetate, respectively, which were converted by hydrolysis to 3,6,8‐p‐menthen‐triol and 8‐hydroxymenthol, respectively.The transformation of racemic menthyl acetate was enantiospecific; (−) 8‐hydroxymenthol that was formed after 21 days contained

ISSN:0233-111X    

DOI:10.1002/jobm.3620310512

出版商:Wiley‐VCH    

年代:1991

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620310513

出版商:Wiley‐VCH    

年代:1991

数据来源: WILEY