摘要:

AbstractRoot nodules ofDerris scandensBENTH., an economic leguminous climbing shrub, contain high amount of IAA, gibberellin like (GA), cytokinin like (CK) and abscisic acid like (ABA) substances. A tryptophan pool present in the nodule may serve as a source of IAA production. Metabolism of IAA in the nodules was evidenced by the presence of IAA metabolising enzymes, IAA oxidase and peroxidase. The symbiont from the nodules, aRhizobiumsp., produced high amount of IAA in culture when supplemented with tryptophan. For IAA production the bacteria preferred L‐tryptophan to DL‐ or D‐isomers of trypt

ISSN:0233-111X    

DOI:10.1002/jobm.3620360502

出版商:Wiley‐VCH    

年代:1996

数据来源: WILEY

摘要:

AbstractThe complete nucleotide sequence of therpoDgene ofEnterococcus faecalisATCC19433 has been determined. This gene encodes a putative 368 amino acids (aa) polypeptide of a predicted Mr of 41842 named sigma 42. Upstream of therpoDgene and beginning from the end of the cloned chromosomal fragment is an open reading frame encoding a putative 264‐aa polypeptide. It is reminiscent of the C‐terminal domain of known DNA primase from Gram‐positive bacteria indicating that theE. faecalis rpoDgene is included in a macromolecular synthesis (MMS) operon, as it was described forEscherichia coli, Bacillus subtilisandLactococcus lactis rpo

ISSN:0233-111X    

DOI:10.1002/jobm.3620360503

出版商:Wiley‐VCH    

年代:1996

数据来源: WILEY

摘要:

AbstractEnterococcus faecalisexhibits an extremely high natural resistance to cadmium which can be even raised by a conditioning treatment at a lower cadmium concentration as well as by a previous exposure to a sublethal temperature. By contrast, thermotolerance is not significantly induced by previous exposure to low cadmium concentration. The synthesis of several proteins is markedly enhanced by a low concentration of the chemical agent.

ISSN:0233-111X    

DOI:10.1002/jobm.3620360504

出版商:Wiley‐VCH    

年代:1996

数据来源: WILEY

摘要:

AbstractAn expression ofnodC promoter ofR. meliloti1021, cloned in front of theEscherichia coli lacZ gene, was used to study the presence ofR. meliloti nodC gene‐inducing compound(s) in extracts and exudates ofA. incanaseeds and roots. The regulatory genenodD was expressed at comparable level in bacterial culture ofR. melilotiwith and without the studied plant extracts and exudates, whereas thenodC‐lacZ fusion expression was increased 4 times by seed exudates of grey alder and 2 times by seed extracts and root ingredients in comparison to thenodC‐lacZ fusion expression inR. melilotigrown in a medium free of plant compounds. Induction ofR. meliloti nodC gene expression byA. incanasubstances was also supported in plant test. Sterile filtrate of coculture ofR. melilotiwith seed exudates ofA. incanainduced root hair deformations and nodule‐like strutures onMedicago

ISSN:0233-111X    

DOI:10.1002/jobm.3620360505

出版商:Wiley‐VCH    

年代:1996

数据来源: WILEY

摘要:

AbstractFluorescentPseudomonassp. strain 267 was evaluated for its ability to stimulate the growth ofA. incana in vitrounder gnotobiotic and nongnotobiotic conditions, as well as in the presence and absence of FeCl3as iron source. Bacterization of grey alder roots withPseudomonassp. strain 267 promoted plant growth under each of the studied conditions. The dry weight of leaves and roots increased significantly when compared with the uninoculated plants. Chlorophyll concentration in the leaves of grey alder, treated with fluorescent pseudomonad, was positively correlated with plant growth stimulation. Its concentration showed 50% increase over the control uninoculated plants. Moreover, it is supposed, thatA. incanagrowth promotion byPseudomonassp. strain 267 may result from secretion of vitamins by this bacterium, which can be utilized by plant.

ISSN:0233-111X    

DOI:10.1002/jobm.3620360506

出版商:Wiley‐VCH    

年代:1996

数据来源: WILEY

摘要:

AbstractSoluble cell‐bound and extracellular cyclodextrin glycosyltransferases ofBacillus maceranswere purified to homogeneity using the subsequent steps of adsorption on starch, ion‐exchange chromatography and gel filtration. The elution profiles and the relative molecular masses of the enzymes as well as their N‐terminal sequences were found to be identical. Moreover, mouse monoclonal antibodies raised against the soluble cell‐bound enzyme recognised equally well both the cell‐bound and the extracellular enzyme preparations. Regarding that no signal peptide could be detected in either the cell‐bound or the extracellular cyclodextrin glycosyltransferase the enzyme was probably entrapped between the cell membrane and the cell wall in the early stationary phase and was released during cell lysis without any m

ISSN:0233-111X    

DOI:10.1002/jobm.3620360507

出版商:Wiley‐VCH    

年代:1996

数据来源: WILEY

摘要:

AbstractLysophospholipids are key intermediates in the metabolism of phospholipids. Cytoplasmic membranes of both eukaryotes and prokaryotes are made of phospholipid bilayers. Phospholipases are activated during phagocytosis. Lysophospholipids generated by phospholipase A2or A1degrade cell membranes and can cause cell lysis. An active lysophospholipase, that hydrolyzes lysophospholipids, was detected by the radioisotope technique inMycobacterium leprae. About two‐thirds of the enzyme was particulate and one‐third cytoplasmic. Optimum activity was at 37 °C, and at pH 6.0. Temperatures above 70 °C completely inactivated the enzyme. The compound AACOCF3, a trifluromethylketone analog of arachiodonic acid, inhibited the activity; the inhibition appeared to be of the uncompetetive type. TheKmof the enzyme was 2.5 × 10−4M, suggesting a fairly strong affinity for the substrate. Lysophospholipids have been shown to be microbicidal to invading organisms. Possession of lysophospholipase byM. lepraeis apparently one of the methods by which the bacilli overcome the defense mechanisms of

ISSN:0233-111X    

DOI:10.1002/jobm.3620360508

出版商:Wiley‐VCH    

年代:1996

数据来源: WILEY

摘要:

AbstractPhylogenetic relationships ofXenorhabdusspp. andPhotorhabdussp. were investigated on the basis of 16S rRNA gene sequences.Xenorhabdusspp. andPhotorhabdussp. were grouped together withProteus vulgarisandArsenophonus nasoniae. This group was distant from other members of the familyEnterobacteriaceae. Xenorhabdus japonicus, previously proposed as a new species, was nearly located toXenorhabdus nematophilus. Signature nucleotides ofX. japonicuswere identified that distinguish it other members of the familyEnterobacteriaceae.

ISSN:0233-111X    

DOI:10.1002/jobm.3620360509

出版商:Wiley‐VCH    

年代:1996

数据来源: WILEY

摘要:

AbstractIn order to investigate the production of auxotrophs of slow growing mycobacterial pathogens, an evaluation of defined liquid and solid media suitable forMycobacterium tuberculosis, Mycobacterium bovisandMycobacterium paratuberculosiswas undertaken. Five defined liquid media were evaluated. A number of solid support matrices was added to the liquid media to produce solid media for evaluation. Tween 80 was added to all media to produce diffuse homogeneous growth. Although satisfactory growth in defined liquid media was achieved with all strains tested, poor growth was generally observed in the equivalent defined solid medium for strains belonging to the tuberculosis complex. OnlyM. paratuberculosisproduced satisfactory growth on defined solid media, especially on Middlebrook 7H9. The inhibitory effect of Tween 80 and the presence of inhibitory factor(s) in agar were confirmed. Partial removal or inactivation of these factors by the addition of absorbents to the media was achieved.

ISSN:0233-111X    

DOI:10.1002/jobm.3620360510

出版商:Wiley‐VCH    

年代:1996

数据来源: WILEY

摘要:

AbstractFour ribosomal proteins of Mr 13 kDa, 15 kDa, 19 kDa and 38 kDa were identified as phosphorylation substrates for protein kinases tightly associated withTrichosporon cutaneumribosomes. It was found that proteins of 13 kDa, 19 kDa and 38 kDa were phosphorylated by multifunctional casein kinase II while the protein of 15 kDa by casein kinase I. Proteins of 13 kDa and 38 kDa were detected in the large subunits while 15 kDa and 19 kDa in the small ribosomal subunits. By using isoelectrofocusing the protein of 13 kDa was resolved into two individual phosphorylated forms. The phosphorylation level of both forms was much higher in ribosomes from the cells collected at the exponential growth phase than in those from the stationary phase. The same phosphoprotein forms were identified in ribosomes fromin vitroandin vivo[32P]‐labelling experiment

ISSN:0233-111X    

DOI:10.1002/jobm.3620360511

出版商:Wiley‐VCH    

年代:1996

数据来源: WILEY