摘要:

Abstract17 spontaneous Gal−mutants ofS. coelicolorwere studied using Southern blottings, but no insertion or deletion mutations were found. 10 mutants ofS. lividansincluded three deletion mutations. The reasons for the lack of insertion mutants are discusse

ISSN:0233-111X    

DOI:10.1002/jobm.3620300502

出版商:Wiley‐VCH    

年代:1990

数据来源: WILEY

摘要:

AbstractOn the basis of structure‐functional analysis of the development ofAcremonium chrysogenum, e.g. under conditions either stimulating antibiotic synthesis or not conductive to production, a scheme was proposed representing the various ways in which morphological differentiation occurs in the culture in dependence on the directions of its metabolism.Three types of culture differentiation were determined. Type 1 differentiation is characterized by the transition of the vegetative stage into the reproductive one with the formation of conidia. Type 2 differentiation is characterized by the formation of typical arthrospores also being the reproductive form. Type 3 differentiation is characterized by the multistage transformation of the mycelium organization into the yeast‐like one which is metabolically more active and is a producer of antibiotics and enzymes.In addition to the defined regularities in the development and differentiation ofAcremonium chrysogenumstructural peculiarities were observed which could be helpful to the search for regulators or specific enzymes taking part in the culture developm

ISSN:0233-111X    

DOI:10.1002/jobm.3620300503

出版商:Wiley‐VCH    

年代:1990

数据来源: WILEY

摘要:

AbstractFour pure methylotrophic bacterial strains isolated from Kuwaiti soil were designated KISRI‐5, KISRI‐6.1, KISRI‐512 and KISRI‐5112, and deposited in NCIMB under NCIB numbers 12135, 12136, 12137 and 12138, respectively. Characterization tests showed that they grew well on methanol and slowly on glucose, at temperatures up to 44 °C and pH 7.0. However, they could not grow on numerous other organic carbon sources. The four strains were very close taxonomically, with minor differences in colony characteristics, lipids pattern, mole G + C% and resistance to certain antibiotics. All strains utilized monomethylamine, but not methane or trimethylamine; all contained ubiquinones with eight isoprene units (Q8), and had C16as their major fatty acid component. Their characteristics indicate that they are different strains of restricted type M facultative methylotrophs, and could be considered new members of the genusMethy

ISSN:0233-111X    

DOI:10.1002/jobm.3620300504

出版商:Wiley‐VCH    

年代:1990

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620300506

出版商:Wiley‐VCH    

年代:1990

数据来源: WILEY

摘要:

AbstractThe activities of fructose‐1,6‐bisphosphatase, malate dehydrogenase and PEP carboxykinase were tested during discontinuous growth of then‐alkane‐assimilating yeastCandida maltosaon glucose or ethanol.As expected, the highest activities of the enzymes were measured in the early log phase of growth on ethanol and the lowest in the early log phase of growth on glucose. However, the differences in the activities are much smaller than inSaccharomyces cerevisiaeand other yeasts under similar conditions. Therefore, we conclude that catabolite repression does not play an essential role in the control of gluconeogenesis inCandida

ISSN:0233-111X    

DOI:10.1002/jobm.3620300507

出版商:Wiley‐VCH    

年代:1990

数据来源: WILEY

摘要:

AbstractThe nucleic acid content inCandida utiliswas autolytically reduced only after previous lyophilization of yeast cells or pretreatment of the cells with ethyl acetate. These pretreatments provided a sufficient permeability of the cell membrane system and thus enabled a reduction of the nucleic acid content. While deep freezing of the cells (−35 °C) had no positive effect on the degradation of nucleic acids, lyophilization of yeast cells led to a 75% decrease of the nucleic acid content. Furthermore, a 55% reduction of the nucleic acid content was proceeded without a concomitant loss of proteins. Similar results were obtained by using ethyl aceta

ISSN:0233-111X    

DOI:10.1002/jobm.3620300508

出版商:Wiley‐VCH    

年代:1990

数据来源: WILEY

摘要:

AbstractThe interactions between polymorphonuclear cells (PMN),Staphylococcus saprophyticuscells and rabbit antibodies againstStaphylococcus aureusV8 serine proteinase or normal rabbit serum proteins were investigated. The effect of opsonization on phagocystosis due to human peripheral polymorphonuclear cells was measured. The results were as follows: phagocystosis index values were relatively increased after the incubation of PMN cells with anti‐serine proteinase γ‐globulin serum fraction, anti‐serine proteinase IgG, non‐immunized rabbit serum or with co

ISSN:0233-111X    

DOI:10.1002/jobm.3620300509

出版商:Wiley‐VCH    

年代:1990

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620300510

出版商:Wiley‐VCH    

年代:1990

数据来源: WILEY

摘要:

AbstractStudies were conducted on the early stages of infection of groundnut leaves byAlternaria alternata.Inoculated leaves were periodically sampled, checked for germination of spores, growth of germ tubes, appressorium formation and method of sporulation. Evidence was observed for the entry of the germ tubes through the stomates (with or without the formation of appressorium‐like structures) or through the host surface by the formation of appressori

ISSN:0233-111X    

DOI:10.1002/jobm.3620300511

出版商:Wiley‐VCH    

年代:1990

数据来源: WILEY

摘要:

AbstractCurrent models for the mechanism of SOS mutagenesis inE. colipropose the involvement of a new or modified DNA polymerase III holoenzyme in error‐prone replicative bypass of bulky DNA lesions assuming an inhibited or excluded 3′ → 5′ proofreading exonuclease function of DNA polymerase. By promotor fusion togalK, gene fusion tolacZand SI analysis thein vivoregulation ofdnaQcoding the proofreading subunit of DNA polymerase III holoenzyme was analyzed under conditions of induced or constitutive SOS expression. The results presented here clearly show that, at least on the level of gene expression no regulatory event seems to contribute to the assumed decrease of proofreading activity during SOS mediated error‐prone bypassing of bulky lesions. On the contrary, an increase indnaQgene expression was observed following treatment with some SOS inducing agents which produces bulky DN

ISSN:0233-111X    

DOI:10.1002/jobm.3620300512

出版商:Wiley‐VCH    

年代:1990

数据来源: WILEY