摘要:

AbstractThe ability of peritoneal cells isolated from different strains of inbred mice to spontaneous interferon production was examined. AKR, C3H, and CBA mice which are known to develop spontaneous diseases such as lymphatic leukemia, mammary tumors or hepatomas in high percentage did not produce physiological interferon. On the other hand, peritoneal cells of BALB/c, 129, DBA/2 mice showed IFN production. In the early phase of their ontogenic development NZB mice were found to produce high titers of the interferon.Differences were found in the contents of a natural IFN‐synthesis‐inhibitor between C3H and NZB mice and are considered as one of the reasons of differentiated physiological interferon synthesis. The disturbance in the regulation of the physiological IFNs synthesis in the development of neoplastic and autoimmune diseases is discus

ISSN:0233-111X    

DOI:10.1002/jobm.3620270602

出版商:Wiley‐VCH    

年代:1987

数据来源: WILEY

摘要:

AbstractThe yeastTrichosporon adeninovoranssecretes an amylase at a high rate if grown in a medium containing starch or maltose. The enzyme was purified 29‐fold by hydroxylapatite chromatography and gel filtration and characterized as a glucoamylase. The enzyme seems to be a glycoprotein with a molecular weight of 225,000. The optimal temperature for the glucoamylase activity was 60 to 70 °C in dependence on the substrate concentration. The enzyme displayed highest activity in a pH range of 4.0–5.5 In the absence of starch or maltose the thermal stability was low but in the presence of substrate a rapid increase of the enzyme stability was observed. TheKmvalues of the enzyme were estimated to be 1.2 g/l for soluble starch and 4 g/l for mal

ISSN:0233-111X    

DOI:10.1002/jobm.3620270603

出版商:Wiley‐VCH    

年代:1987

数据来源: WILEY

摘要:

AbstractBiosynthesis of polygalacturonase (PG) byA. nigerstrain R 1/214 correlates with the morphology of mycelium in submerged culture. The mean specific PG‐synthesis (PG‐U · g−1· h−1) increases with the degree of compactness of mycelium. PG‐production can be optimized by a precise adjustment of the culture conditions after direct spore inoculation (diffuse mycelium) but the high synthesis as by compact mycelium is never obtained. Different reasons for the higher enzyme production by the pellet mycelium are discussed. PG‐synthesis is assumed to be strictly connected with a limitation of nutrient and

ISSN:0233-111X    

DOI:10.1002/jobm.3620270604

出版商:Wiley‐VCH    

年代:1987

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620270606

出版商:Wiley‐VCH    

年代:1987

数据来源: WILEY

摘要:

AbstractBacterial cultures were selected from the native flora of liquid manure in order to metabolize liquid manure substances. The mixed culture used in the growth experiments is characterized by low growth rates when maximum degradation of acetate occurs. The biomass concentration reached 2.2 g/l.

ISSN:0233-111X    

DOI:10.1002/jobm.3620270607

出版商:Wiley‐VCH    

年代:1987

数据来源: WILEY

摘要:

AbstractTwo basidiomycetes cultures, BH1and BW1, isolated from the biomanure sample of a bio‐gas plant belonging toPolyporussp., were used in combination with celluolytic cultures viz.Trichodermasp. andCellulomonasstrain R2 for microbial protein production from whole bagasse. The biomass yields obtained were much higher in mixed cultures fermentation than in corresponding controls. Combination of BH1/BW1withCellulomonasproved to be the better in comparison withTrichoderma. Simultaneous inoculation of the bacteria along with BH1culture resulted in higher protein productio

ISSN:0233-111X    

DOI:10.1002/jobm.3620270608

出版商:Wiley‐VCH    

年代:1987

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620270610

出版商:Wiley‐VCH    

年代:1987

数据来源: WILEY

摘要:

AbstractFor mass culture ofEscherichia coliK1a fermenter dialysis culture technique based on an artificial hollowfiber kidney and substrate feeding is described and compared with conventional shaking culture and fermenter batch culture techniques.Important growth parameters as bacterial dry weight, optical density and live cell counts demonstrate the advantage of this new method. On the basis of bacterial yields, effectivity had increased about 100 fold. The synthesis of K1capsular polysaccharides, which is influenced by a number of factors, proved to be good. Thus it was possible to obtain the quantities of native capsular substances necessary for bed‐side diagnostics and the investigation of this virulence marke

ISSN:0233-111X    

DOI:10.1002/jobm.3620270611

出版商:Wiley‐VCH    

年代:1987

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620270613

出版商:Wiley‐VCH    

年代:1987

数据来源: WILEY

摘要:

AbstractThe protoplast fusion of auxotrophicPenicilliummutats after dielectrophoretic cell contact and rectangular field pulses (4,2 kV/cm, 25 μs) was microscopically observed in a microchamber consisting of two vapour‐plated chromium electrodes on a microslide (gap between the electrodes of about 150 μm). The conditions for protoplasting ofPenicilliumand for electrofusion are described. Isolation of viable fusion products was reali

ISSN:0233-111X    

DOI:10.1002/jobm.3620270614

出版商:Wiley‐VCH    

年代:1987

数据来源: WILEY