摘要:

AbstractWe isolated stable mutants fromTrichosporon adeninovoransresistant to 2‐deoxy‐D‐glucose. All mutants were altered in the C‐catabolite repression and produced different amounts of glucoamylase and β‐glucosidase when grown on several carbon sources. In addition to the C‐catabolite derepression of these extracellular enzymes, a repression defect of isocitrate lya

ISSN:0233-111X    

DOI:10.1002/jobm.3620290202

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

摘要:

AbstractSpirochetal organisms were sought in 18 skin and 4 synovial membrane specimens obtained by biopsy from 22 Lyme disease patients. The presence of spirochetes in body tissues was histologically demonstrated in one patient with lymphadenosis benigna cutis, one patient with acrodermatitis chronica atrophicans and in one patient with active arthritis. The organisms were 5–30 μm long and 0.12–0.25 μm thick, had 8 or 11 flagella arising from both ends of the body, and their ultrastructure was analogous to that of culturedBorrelia burgdorferistrains. They were located intra‐ or perivascularly, or in the collagenous connective tissue of the skin and synovium. This implies that Lyme spirochetes may have a potential to survive in body tissues and cause injury to blood

ISSN:0233-111X    

DOI:10.1002/jobm.3620290203

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620290205

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

摘要:

AbstractArthrobacter oxydans317 which is able to bring about the complete degradation of β‐sitosterol was treated with acridine orange to obtain a plasmid cured strainArthrobacter317AL, incapable of producing steroid ring degradation. The strain was neither able to form 4‐androstene‐3,17‐dione(AD) from β‐sitosterol nor to convert AD to 1,4‐androstadiene‐3,17‐dione(ADD). It was, however, able to hydroxylate AD and ADD at the C‐17 position to form testosterone and 1,4‐androstadiene‐17β‐ol‐3‐one respectively. The strain could grow on β‐sitosterol presumably utilizing the degradation products of the sterol side chain. Partial degradation of the β‐sitosterol side chain was demonstrated by the isolation of 27‐norcholest‐4‐en‐3,24‐dione, a metabolic product of the side chain degradation. The existence of metabolic blocks in a further degradation of the side chain to AD, in 1,2‐dehydrogenation and 9α‐hydroxy

ISSN:0233-111X    

DOI:10.1002/jobm.3620290206

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

摘要:

AbstractThe constitutive MLS resistance plasmid pE3692 (2.4 kb) fromS. aureusand pE3692‐9 plasmid derived from pE3692 byin vitrodeletion of the smallHindIII‐HindIII fragment Sta t (0.3 kb) were introduced intoS. aureusRN450 andB. subtilisUOT0277 strains. The pE3692 plasmid was completly stable inS. aureusRN450, however, inB. subtilisUOT0277 this plasmid was segregationally unstable. Deletion of the Sta t fragment from the pE3692 reduced plasmid stability considerably both inS. aureusandB. subtilishost strains. The lower expression of tylosin‐resistance of theermgene of the pE3692‐9 plasmid inB. subtilisUOT0277 strain was also o

ISSN:0233-111X    

DOI:10.1002/jobm.3620290207

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

摘要:

AbstractWe reviewed our investigations on sexual behaviors and interactions including sexual cell agglutination and pheromone action mainly in non‐conventional yeasts,Hansenula anomala, H. wingei, Pichia amethionina, P. heedi, P. opuntiae, Saccharomyces kluyveri, S. globosus, S. exiguus, Saccharomycodes ludwigii.The techniques and genetic models including the cassette model and α1‐α2 hypothesis which had been developed largely inS. cerevisiaewere applicable to these yeasts in principle. The sexual agglutination was distinctly species‐specific while sex pheromones were cross‐reactive beyond species' barriers. The successful induction of heterothallic strains from homothallic strains inS. exiguusby mutagenesis enabled to the subsequent biochemical and genetical analysis of sexual behavior in the yeast. The phylogenetic consideration on sex differentiation is also

ISSN:0233-111X    

DOI:10.1002/jobm.3620290208

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620290201

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY