摘要:

AbstractThe activities and interrelations of the processes of methanogenesis and methane oxidation were investigated in the water and sediment of the middle Saale River. The studies showed that in the impounded reaches of the river, which is heavily polluted by organic waste and inorganic compounds, sediments occur with high methane production rates (6.3–64.6 mmol/m2· h). The methane oxidation in the surface sediment layer (20–240 μmol/1 · h) was shown to function as an effective barrier for the methane diffusion out of the sediment. The methane concentrations in the anaerobic sediment were very high (383–1696 μmol/1), but low in the water (0.33–9.3 μmol/1) in consequence of oxidation processes and water turbulence. The distribution of methane‐oxidizing bacteria was studied. High cell concentrations were found (water, 8.8 · 101— 2.8 · 104cells/ml; sediment, 1.3 · 106— 1.3 · 107cells/gram). The ecological importance of the processes of the methane cycle was evaluated by calculation of the oxygen demand (methane oxidation) and of the mineralization capacity in the river system. The anaerobic mineralization of organic matter with the methanogenesis as terminal step amounted to 59–87% of the total mineralization. The sediment and consequently the particular organic load was found to be a significant factor for estimation and control of the wa

ISSN:0233-111X    

DOI:10.1002/jobm.3620290402

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620290404

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

摘要:

AbstractA simple procedure was developed for the laboratory production and purification of gramme quantities of T‐2 toxin. After growing at 22 °C on rice for two weeks,Fusarium sporotrichioidesT‐21 produced average T‐2 toxin concentrations of 1309 mg/kg and as much as 1000 mg of crystalline product were recovered. The method described avoids long‐term, low temperature incubations. Crude culture extracts were rapidly purified in two steps by column chromatography on silica gel. The purity of the crystallized product was verified by thin‐layer chromatography using various spray reagents for

ISSN:0233-111X    

DOI:10.1002/jobm.3620290405

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620290407

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

摘要:

AbstractThe exact chemical characterization of the preparedEscherichia coliK 1 capsule polysaccharide is necessary and a prerequisite for using this antigen as a screening antigen in the production of monoclonal anti‐K 1‐antibodies.The K 1‐antigen, prepared by phenol‐water‐extraction, was analyzed by protein, RNA, and neuraminic acid determination. An addition, the antigen was subjected to elementary analysis, infrared‐ and13C‐NMR‐spectroscopy, and gelchromatography.After testing the serological specificity, the K 1‐antigen was identified as a high molecular po

ISSN:0233-111X    

DOI:10.1002/jobm.3620290408

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

摘要:

AbstractExternal potassium concentration was measured in suspensions of washed living mycelia ofStreptomycesstrains. An active uptake of K+by the mycelium was observed from the medium at proper aeration and stirring.The endogenous regulatory protein factorC.in very low concentrations (down to 0.7 ng ml−1), induced in a specific way the release of K+. Changes in the electrolyte composition of the medium had moderate effect on the factorCinduced K+release. FactorCproved to be active in nine differentStreptomycesstrains. OnlyS. griseus45H, the producer of factorC, did not respond. Response to factorCwas not affected by chloramphenicol or actinomycin D. The process followed a “slow kinetics” with 3–10 min latency of K+release.It was assumed that the factorCinduced K+release could be the consequence of a more rapid process realized at the level of existing macromo

ISSN:0233-111X    

DOI:10.1002/jobm.3620290409

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

摘要:

AbstractA new anthracycline, aclacinomycin ZIMET 33352, has been isolated from cultures ofStreptomycesspec. AM 33352/NG 3–1

ISSN:0233-111X    

DOI:10.1002/jobm.3620290410

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620290412

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

摘要:

AbstractLikeP. syringaepv.phaseolicola, other pathovarieties of thePseudomonas syringaegroup (P. syringaepv.glycinae, P. syringaepv.syringae, P. syringaepv.tabaci, andP. syringaepv.tomato) possess two sucrose splitting enzymes, a constitutive levansucrase found both extracellularly and cellularly, and a cellular sucrase, the synthesis of which is regulated differently. InP. fluorescens, a closely related saprophytic pseudomonad, only a levansucrase was found.

ISSN:0233-111X    

DOI:10.1002/jobm.3620290413

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY

ISSN:0233-111X    

DOI:10.1002/jobm.3620290415

出版商:Wiley‐VCH    

年代:1989

数据来源: WILEY