摘要:

An immuno-nephelometric me- thod, in a continuous flow system for the determination of plasma fibrinogen is described. The method is specific, rapid (60 determinations per hour), requires only 0.03 ml of plasma and compares favourably (r = 0.923 ± 0.038) with Leclerc’s colorimetric meth

ISSN:1424-8832    

DOI:10.1159/000213988

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

A screening test has been investigated for detecting factor VIII antibodies, based on the observation of Strauss and Merler that a lower initial concentration of factor VIII detects weaker antibodies. At an initial factor VIII concentration of 0.1 β/ml weak antibodies are detected which would not be revealed at the higher concentration of factor VIII normally employed. The most suitable sources of factor VIII are cryoprecipitate and normal human plasma, whilst human factor VIII is less satisfactory

ISSN:1424-8832    

DOI:10.1159/000213989

出版商:S. Karger AG    

年代:1972

数据来源: Karger

摘要:

Lyophilized or frozen plasmas with congenital deficiencies of factors II, VII, IX, or X, abnormal factor X (factor X Friuli) plasma, absorbed normal plasma, plasmas from patients treated with coumarin anticoagulants and normal plasma were studied in a crossover electrophoresis (electrosyneresis) system using an anti-factor X antiserum. A factor X band was present in plasmas with a deficiency of factors II, VII, and IX, in abnormal factor X plasma, and in normal plasma. No difference in the position of the factor X band was noted for all these plasmas. In coumarin plasma, two factor X bands were evident. The first of these two bands had the same position as the normal factor X band; the second one migrated slightly more towards the cathode. In adsorbed normal plasma and in factor X deficient plasma, no bands were seen. Minor, lighter bands were seen in most plasmas on the cathodic side of the slide and thought to represent secondary activities of the antiserum. These results suggest that the abnormal factor X (factor X Friuli) has the same crossover electrophoretic mobility as normal factor X, whereas the coumarin-induced abnormal factor X has a different mobility.

ISSN:1424-8832    

DOI:10.1159/000213990

出版商:S. Karger AG    

年代:1972

数据来源: Karger