摘要:

Clinical and experimental observations suggest that tumor-induced endothelial cell (EC) injury may be one of several initial events in the establishment of tumor metastases. This work investigates tumor-induced EC injury and the interaction between tumor-damaged EC and platelets. We used cultured bovine EC and extracts of four cultured human malignancies. EC injury was assessed by 51Cr and lactic dehydrogenase (LDH) release. Incubation of EC with melanoma, breast carcinoma or lung carcinoma caused significant LDH and 51Cr release, whereas colon cancer seemed ineffective. Increased adhesion of platelets to tumor-injured EC was noted. These observations indicate that certain varieties of tumor cause EC injury. Adhesion of platelets to tumor-injured EC results in the formation of platelet-tumor thrombi at the endothelial surface, an event that may initiate tumor invasion of the vessel wall.

ISSN:1424-8832    

DOI:10.1159/000215751

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

A Cobas Bio® centrifugal analyzer was used in a clinical laboratory for the performance of chromogenic clotting assays. Three commercially available photometric clotting tests – prothrombin time (PT), activated partial thromboplastin time (aPTT) and fibrinogen – were compared with the traditional clotting assays during 3 months. No great discrepancies were found between the traditional assays and the new photometric assays. The chromogenic PT could replace the traditional thrombotest®, PT and Normotest®, because it was sensitive and accurate over a broad range of clotting factor activity. Furthermore the chromogenic PT could be used to discriminate between a decreased clotting activity due to vitamin K deficiency or to a decreased protein synthesis by the liver. A decreased protein synthesis was confirmed by measuring a decrease in the serum cholinesterase activity. The chromogenic aPTT could be used for the assay of heparin concentrations in the therapeutic range and turned out to be more sensitive for deficiencies of factor VIII and factor IX than a traditional clotting aPTT. We conclude that the accuracy and practicability of clotting assays are improved by the new assays without diminishing the clinical value of the r

ISSN:1424-8832    

DOI:10.1159/000215752

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Spectrophotometric clotting assays using chromogenic substrates were adapted for use in a routine laboratory batch analyzer. Contrary to another described method the same plasma dilution can be used for each of the different coagulation parameters. Optimal assay conditions were determined. The within- and between-assay variation, the intraindividual variation during 1 day and from month-to-month were determined; the chromogenic assay was compared to a clotting assay. No significant difference (p < 0.01) between manual and automated methods was found.

ISSN:1424-8832    

DOI:10.1159/000215753

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Twenty-three patients with congenital factor VII (FVII) deficiency, belonging to 9 kindreds were studied. Immunological variants were classified according to the relationship between FVII coagulant activity (FVIIC) and the level of FVII antigen (FVIIAg), considering 3 previously described groups: VII-, VII+ and VIIR. Activation variants were determined by the reactivity pattern with three different thromboplastins. One patient was classified as VII-, 16 as VII+, and 6 as VIIR. Three patients belonging to the same kindred showed a Padua 2 FVII deficiency, and 1 patient showed a Padua 1 variant. There was no correlation between antigenic or activation variants and severity of bleeding tendency. Classical autosomal recessive mode of inheritance was observed in VII- and VII+ families. Nevertheless, a possible autosomal dominant trait was observed in VII+ kindreds.

ISSN:1424-8832    

DOI:10.1159/000215754

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

We describe the clinical history of a 35-year-old woman with severe cross-reacting-material-negative factor VII deficiency. Because of a severe hemophilic arthropathy of the hips, reconstructive surgery was indicated. After advice was obtained from 11 clinical hemostasis experts it was decided to use plasma for factor VII replacement during and after surgery and to use pneumatic boots to prevent thrombosis. Volume overload was treated with plasmapheresis and diuretics. The operation was carried out without abnormal blood loss and the patient recovered without thromboembolic complications. We conclude that continuous infusion of plasma is a suitable way to replace factor VII provided adequate venous access is available for plasmapheresis.

ISSN:1424-8832    

DOI:10.1159/000215755

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

In severe von Willebrand’s disease (vWD), the multimeric analysis of von Willebrand factor (vWF) has been hindered by the low content of the protein although different patterns have been observed by radiocrossed immunoelectrophoresis (RCIE). Cryoprecipitate and vWF concentrate were obtained from the plasma of a severe vWD patient, that on RCIE had only the more anodic forms. The sodium dodecylsulfate (SDS) agarose analysis evidences the presence of the main bands, a relative increase of the smaller forms and a lesser definition of some minor bands. These findings suggest the possibility of structural abnormalities in severe vWD. The study of cryoprecipitates could be a means to evidence the presence of multimers and the extent of the eventual pattern variabilit

ISSN:1424-8832    

DOI:10.1159/000215756

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

The metabolism and urinary excretion of 125I antithrombin III (AT III) was investigated in 2 patients with a nephrotic syndrome caused by minimal chain nephritis and primary amyloidosis, and acquired deficiency of AT III. Increased AT III catabolism was observed in both patients, even after correction for urinary protein loss. Increased AT III catabolism was due to increased influx from the extra- to the intravascular compartment in 1 patient, and to an increased fractional catabolic rate in the other patient who developed later a pulmonary embolism. Analysis of urine samples revealed biologically inactive whole AT III molecules and biologically as well as antigenically inactive fragments, respectively, whereas daily plasma gel filtration showed intact radioactive AT III. These observations reject the hypothesis that AT III deficiency in nephrotic patients is only due to urinary loss of AT III.

ISSN:1424-8832    

DOI:10.1159/000215757

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

We have done a comprehensive study in normal volunteers of the in vitro and ex vivo effects of the antiplatelet agent indobufen on platelet aggregation, the release reaction and thromboxane B2 (TxB2) production as induced by different concentrations of aggregating agents. At low concentrations (10 μM), indobufen completely inhibited secondary platelet aggregation, the release reaction and TxB2 production stimulated by ADP, epinephrine and low concentrations of platelet-activating factor (PAF acether). Higher concentrations of indobufen (100 μM) completely inhibited TxB2 production, platelet aggregation and ATP release induced by arachidonic acid (1 mM) or collagen (2 μg/ml). The inhibitory effect was partially overcome by higher concentrations of arachidonic acid (2 mM). Data obtained ex vivo 2 h after the oral administration of 200 mg indobufen to 8 normal volunteers were in keeping with those of the in vitro study. We conclude that indobufen inhibits platelet aggregation and the release reaction by inhibiting the platelet arachidonate pathw

ISSN:1424-8832    

DOI:10.1159/000215758

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

In order to investigate whether α2-antiplasmin (α2-AP) levels may be related to thrombin activity, we measured α2-AP and fibrinopeptide A (FPA) in 51 patients with clinical conditions frequently associated with increased thrombin activity. The diagnoses were: atherosclerotic disease, chronic inflammatory disease and hematological neoplastic disease. A significant negative correlation was found between α2-AP and FPA (p < 0.01). When patients were divided into three subgroups on the basis of their FPA levels, a significant reduction in α2 9 ng/ml). Accordingly, a significant negative relationship between α2-AP and FPA was found only in this subgroup (p < 0.01). Our data suggest that the partial consumption of α2-AP in patients with elevated FPA levels may reflect a subclinical fibrinolysis activation secondary to increased thrombin ac

ISSN:1424-8832    

DOI:10.1159/000215759

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Basal venous blood levels of several components of the fibrinolytic enzyme system (plasminogen activator, plasminogen, fibrin degradation products, α2-antiplasmin, and α2-macroglobulin) were measured in 100 white men with angiographically defined coronary artery disease. The tests of fibrinolysis were not related to the severity of coronary artery disease, as indicated either by the number of vessels involved or by a coronary score system. Fibrinogen levels, however, did show a modest association with the extent of coronary atheroma (r = 0.21, p < 0.05). Triglyceride levels were associated with the inhibitors of fibrinolysis: positively with α2-antiplasmin (r = 0.31, p < 0.005) and negatively with α2-macroglobulin (r = -0.25, p < 0.05). The α2-antiplasmin levels were significantly elevated in patients with hypertriglyceridaemia. Neither smoking nor β-blockade had any effect on the tests of fibrinolysis. This study adds support to the association of plasma fibrinogen with ischaemic heart di

ISSN:1424-8832    

DOI:10.1159/000215760

出版商:S. Karger AG    

年代:1987

数据来源: Karger