摘要:

Prostaglandins play an important role in the platelet-vessel wall interaction. The inhibition of PGI2 synthetase results in an increase of platelet thrombosis induced by adeno-sine diphosphate. Addition of exogenous arachidonic acid further increases the phenomenon. The ratio of cyclic endoperoxides (PGG2 and PGH2) to prostacyclin (PGI2) could well be the determining trigger in platelet-vessel wall interaction and local thrombosis.

ISSN:1424-8832    

DOI:10.1159/000214653

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Adenosine diphosphate (ADP) caused rapid and reversible aggregation of turkey thrombocytes, as determined by optical and microscopical examination. To investigate the binding of ADP to these cells, turkey thrombocyte-rich plasma was incubated with ADP-8–14C or ADP-3–3H and with human serum albumin labeled with 125I. The thrombocytes were rapidly separated by centrifugation through silicone oil. From radioactivity determination of plasma and thrombocyte pellets the uptake of ADP radioactivity by thrombocytes was calculated. Scatchard analysis of concentration-dependent binding yielded a hyperbolic plot which indicates that turkey thrombocytes similarly to human platelets have more than one type of binding site for

ISSN:1424-8832    

DOI:10.1159/000214654

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

In a prospective randomized clinical trial involving 150 patients who underwent major abdominal surgery, acetylsalicylic acid (ASA) and dihydroergotamine (DHE) were examined either alone or in combination for their potential to prevent thromboembolism. Combined treatment with ASA and DHE was found to be more effective in preventing the spread of thrombi and their propagation to the femoral veins.

ISSN:1424-8832    

DOI:10.1159/000214655

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Measurements of total proteins, myosin, actin, actin-binding protein, and ATPase activity of myosin were examined in platelets from patients with idiopathic scoliosis and from healthy individuals. Abnormalities in the distribution of total and contractile proteins were revealed after frac-tionations. The insoluble fraction of the patients’ platelets had a higher, and the cytosol fraction had a lower than normal protein content. Similar differences were observed in the specific activity of myosin ATPase. These findings show that in patients with idiopathic scoliosis platelet defects exist and that their study might be useful in research of the diseas

ISSN:1424-8832    

DOI:10.1159/000214656

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The influence of brinase on fibrinogen levels, ethanol gelation test (EGT), fibrin content of lungs and kidneys (125I-fibrinogen) and brinase inhibitor capacity was investigated in the rat. The animals were either treated with i.v. infusions of brinase (4 mg/kg), tranexamic acid (AMCA) + brinase or heparin + AMCA + brinase. A control group was given i.v. saline, a reference group AMCA + thrombin. Brinase caused a decrease in fibrinogen levels and an increase in the incidence of positive EGT, deposition of fibrin was not observed. Infusion of brinase into rats with inhibited fibrinolytic system (AMCA), caused a more pronounced decrease in fibrinogen levels, a further increase in the incidence of positive EGT and also fibrin deposition in the kidneys. In rats treated with AMCA + thrombin a similar decrease in fibrinogen levels was recorded, the EGT was positive in all animals, and extensive fibrin deposits were observed in the kidneys and lungs of the animals. Heparinization before infusion of AMCA + brinase antagonized the lowering in fibrinogen levels and the increase in incidence of positive EGT and, moreover, fibrin deposits were no longer observed.

ISSN:1424-8832    

DOI:10.1159/000214657

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The direct antiglobulin consumption (DAC) test, used for the detection of platelet autoantibodies, was found to be positive in 86% of a group of 150 patients with thrombocytopenic purpura. The platelet radioactive Coombs’ (DRC) test was performed in 30 patients and the correlation between the two tests was statistically significant (r = 0.48, p < 0.01). The two tests were used in an indirect mode for the detection of antiplatelet antibodies in serum. The results of the two tests were in good agreement (r = 0.66, p < 0.001). There was not a statistically significant correlation between the level of platelet-associated IgG and the presence of platelet antibodies in the serum. These results indicate that the DAC test or DRC test can be used interchangeably for the detection of antibody in serum or of antibody bound to platelets, and that only the direct test, using the patients’ platelets, is useful in the diagnosis of autoimmune thrombocytopenic purp

ISSN:1424-8832    

DOI:10.1159/000214658

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

3 young Japanese sisters with congenital α2-plasmin inhibitor (α2-PI) deficiency are reported. They have mild umbilical bleeding and/or repeated prolonged bleeding after minor trauma, but rarely spontaneous bleedings. The most characteristic hemostatic findings were shortened whole blood clot lysis time and euglobulin lysis time. Activities of all hemostatic factors except α2-PI were within normal range. Both functional and immunological absence of α2-PI were found in the plasma, and this failure to detect α2-PI was not corrected by the addition of the patient’s plasma of the first described case of α2-PI deficiency. Clinical and laboratory data revealed that these patients were probably homozygous for α2-PI deficiency and born of heterozygous parents, but not of consanguineous ones. Bleeding episodes due to deficiency of (α2-PI in these patients were well controlled by an antifibrinolytic agent, tranex

ISSN:1424-8832    

DOI:10.1159/000214659

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

A patient with a peculiar factor VII is described. The propositus is a 70-year-old man without any bleeding tendency. The coagulation pattern is characterized by a prolonged rabbit brain prothrombin time, a normal Stypven cephalin clotting time and a normal throm-botest. Factor VII activity is low when assayed using rabbit brain thromboplastin but is normal when assayed using ox brain thromboplastin. The neutralization test performed with an antifactor VII antiserum revealed a normal factor VII antigen level. A pedigree study has not been possible, the patient having no living relatives. No differences were observed between the biological results of our patient and those described by Girolami as factor VII + Padua.

ISSN:1424-8832    

DOI:10.1159/000214660

出版商:S. Karger AG    

年代:1982

数据来源: Karger