摘要:

Levels of coagulation factors I, II, V, VII-X, VIII, IX, X, plasminogen and antithrombin III, were determined at midcycle in 17 test subjects using oral contraceptive agents, and in 10 control subjects. Immunoassay of factor II and antithrombin III was also carried out, and platelet aggregation quantitatively assessed. Significant increases were found in the activity of factors II, V, VII-X, X and plasminogen, but factor II antigen levels did not change. Platelet aggregation with collagen was significantly enhanced. No change occurred in antithrombin III activity or antigen levels.

ISSN:1424-8832    

DOI:10.1159/000214089

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

The activity of factors II, VII, VIII, IX, X and fibrinogen and factor VIII antigen levels were followed during a single menstrual cycle in three females using oral contraceptive agents, and in two controls. Marked cyclical variation occurred in the activity of factor VII and VIII, but not in factor VIII antigen levels. Less marked changes occurred in factor II and fibrinogen.

ISSN:1424-8832    

DOI:10.1159/000214090

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

It is confirmed that metabolic energy is a prerequisite for the uptake of serotonin by human blood platelets. Starvation (i.e. incubation at 25 °C of platelets resuspended in glucose-poor autologous plasma) generally stops glucose consumption, lactate formation as well as serotonin uptake within 2 h. The addition of glucose restores lactate formation immediately and, independent of total starvation time, to normal or even higher levels. The active uptake of serotonin, however, can be restored only partly after prolonged incubation with glucose, to an extent which seems to be dependent of the preceding starvation time. The results are compatible with the hypothesis that besides an active carbohydrate metabolism another factor, presumably the integrity of the platelet membrane, is obligatory for the active transport of serotonin

ISSN:1424-8832    

DOI:10.1159/000214091

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

In a prospective randomised trial, three groups of 20 patients each were compared. A first group served as a control, a second group received dipyridamole and acetylsalicylic acid and a third group received low doses of heparin. The incidence of deep vein thrombosis determined by the 125I-fibrinogen test was 40 % in the first group, 50 % in the second group, and 5 % in the third group. The differences between the heparin group and the two other groups are statistically significant. No wound complication and no haematoma at the injection site occurred.

ISSN:1424-8832    

DOI:10.1159/000214092

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

A simple assay procedure for antithrombin III is described. The synthetic product benzoyl-Phe-Val-Arg-p-nitroanilide (S-2160) is used as substrate. By thrombin p-nitroaniline is split from the tripeptide molecule. The yellowish color of this split product can be measured in a photometer. Inactivation of thrombin by antithrombin III results in inhibition of this reaction. The percentage of thrombin inhibited is in direct relation to the activity of antithrombin III. Various modifications were tested to optimate the method. Comparable results were obtained between the coagulation method of HEnsen and LOeliger and the photometric method.

ISSN:1424-8832    

DOI:10.1159/000214093

出版商:S. Karger AG    

年代:1975

数据来源: Karger

摘要:

A modification of the fibrin plate method is presented. Plasminogen-free human fibrinogen and plasminogen purified by affinity chromatography have been used. Fibrin plates without and with a constant amount of plasminogen and with agarose as stabilizing medium were used for the estimation of plasmin and plasminogen activator activity. Activator activity could be demonstrated in sterile bile and saliva. When plasmin activity was present, estimations of plasminogen activator were approximate. The method is sensitive, small volumes of reagents and samples are needed. The error of the method is comparatively low and the reproducibility is good.

ISSN:1424-8832    

DOI:10.1159/000214094

出版商:S. Karger AG    

年代:1975

数据来源: Karger