摘要:

Factor VII activity and factor VII cross-reacting material (CRM) in plasma of patients with liver cirrhosis have been studied before and after vitamin K1 parenteral administration. Subjects were divided into two groups according to the absence (group I) or the presence (group II) of the following clinical findings: ascites, portal hypertension, encephalop-athy. Factor VII activity and CRM show a statistically significant correlation (p < 0.001) in all patients. In group II, significantly reduced levels of both activity and CRM were found as compared to the reference and the group I values. No variations were found after vitamin K administration. Different thromboplastins, investigated with respect to their sensitivity for factor VII, acted differently. Patients with normal albumin levels also showed normal levels of factor VII activity and antigen. No correlation was found in group II. The data discussed suggest that in liver cirrhosis with unknown aetiology no immunologically detectable precursor of factor VII is present.

ISSN:1424-8832    

DOI:10.1159/000214643

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

12 plasmaphereses were carried out in 5 patients with antibodies to F VIII (3 haemophilic antibodies; 2 spontaneous antibodies). Plasmapheresis led in all instances to a marked reduction of the antibody level and there was a good correlation between the amount of plasma exchanged and the decrease of the antibody level. About 40 ml of plasma/kg body weight have to be removed to reduce the antibody level to half. In patients with low titre antibody who need treatment for serious bleeding, plasmapheresis is a more rapid and less expensive procedure than neutralisation of the inhibitor by high doses of F VIII. In one haemophiliac repeated plasmaphereses and subsequent high dose F VIII treatment eliminated the antibody within a short time. Plasmapheresis should always be considered when patients with antibodies to F VIII have to be treated because of severe bleeding.

ISSN:1424-8832    

DOI:10.1159/000214644

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

The levels of factors VIII, II, and VII rise during pregnancy in normal women. In addition, increases in factor VIII levels have been observed in pregnant carriers of hemophilia A and in women affected with von Willebrand’s disease. The influence of pregnancy on factor IX levels is less clear. Consequently, we determined serial factor IX coagulant activities (IX:C) and factor IX antigen levels (IX:Ag) during the pregnancy of an affected carrier of hemophilia B who had baseline values of 13 % for both IX:C and IX:Ag levels. Neither level rose during pregnancy and the patient was treated with plasmapheresis and plasma infusions during the delivery and the postpartum period. Excessive bleeding did not occu

ISSN:1424-8832    

DOI:10.1159/000214645

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

8 young infants aged 14 days to 5 months with vitamin K-dependent factor deficiency were studied with special reference to prothrombin activity and antigen level. Among them, 3 infants had congenital bile duct atresia and 5 were breast-fed babies with severe hemorrhagic tendency of unknown cause. In the patients with both congenital bile duct atresia and breast feeding the ratio of prothrombin activity to prothrombin antigen was lower than 0.1. Furthermore, the arc of prothrombin antigen in these patients demonstrated a faster anodal shift than did normal prothrombin antigen on crossed immunoelectrophoresis. This abnormal prothrombin antigen was not consumed after recalcification of patient plasma, and adsorbed poorly on BaSO4. In addition, the abnormal prothrombin antigen disappeared from the patient plasma within a few days after parenteral administration of vitamin K. These results suggest that this abnormal prothrombin is PIVKA-II.

ISSN:1424-8832    

DOI:10.1159/000214646

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Modifications are described to the methods of extraction of phospholipids from brain tissue which were successful in diminishing the oxidative changes taking place during extraction. The reagents produced, particularly when prepared by a modified Folch procedure, were stable and had reproducible coagulant activities.

ISSN:1424-8832    

DOI:10.1159/000214647

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

We studied platelet survival in 14 cardiac patients before heart valve replacement using 75Se-methionine ‘cohort label’. The temporal variation of plasma and platelet activity was plotted by a continuous curve by means of a computerized interaction of the single points. Then we computed the plasmatic radioactivity fraction used daily for thrombo-poiesis and made a subtraction of this aliquot from the experimental activity in the catabolic phase of the slope. Platelet life span obtained after this ‘correction’ is comparable with the values of random label using 51<

ISSN:1424-8832    

DOI:10.1159/000214648

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

Platelets were labelled with surface-labelling agents which identified up to six platelet membrane proteins. Three of these labelled proteins were recognised by a specific anti-platelet membrane serum by immunoprecipitation. Affinity fractionation of platelets with insolubilised IgG obtained from the anti-platelet membrane serum indicated a greater reaction between the antiserum and the platelet surface. Fab fragments from the antiserum inhibited ristocetin-induced aggregation of platelets and partially inhibited collagen-induced aggregation but did not affect ADP aggregation. Crossed immunoelectrophoresis of platelets using the anti-platelet membrane serum showed that the antiserum was specific for platelet membranes.

ISSN:1424-8832    

DOI:10.1159/000214649

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

A group of patients with myeloproliferative disorders was studied with respect to platelet aggregation responses, release of β-thromboglobulin and incorporated 5-hydroxy-tryptamine, and synthesis of thromboxane B2. In all patients the resting plasma β-thrombo-globulin was elevated. Aggregation responses were frequently impaired to adrenaline, arachi-donic acid, A23187 and the prostaglandin endoperoxide analogue, U44069. Both 5-hydroxy-tryptamine and β-thromboglobulin release were greater with patients’ platelets than with those of controls in response to adrenaline, ADP and U44069. The patients’ platelets produced more thromboxane B2 than did controls, irrespective of the agonist used, yet those aggregating agents which are thought to act by generating thromboxane A2 were relatively ineffective in causing aggregation. This might reflect resistance to thromboxane A2 action in these patients, which is met by increased thromboxane for

ISSN:1424-8832    

DOI:10.1159/000214650

出版商:S. Karger AG    

年代:1982

数据来源: Karger

摘要:

n-Acetylneuraminic acid (NANA) was measured in the platelets and serum of normal subjects, patients with malignant disease, and patients with acute myocardial infarction. The malignant disease group had a mean platelet NANA 24% below normal and a serum NANA 68% above normal. Both results were highly significant. Samples from patients with acute myocardial infarction showed slightly reduced platelet NANA and slightly raised serum NANA, neither change being significant. There was no correlation between platelet and serum NANA in the neoplastic group.

ISSN:1424-8832    

DOI:10.1159/000214651

出版商:S. Karger AG    

年代:1982

数据来源: Karger

ISSN:1424-8832    

DOI:10.1159/000214652

出版商:S. Karger AG    

年代:1982

数据来源: Karger