摘要:

The anticoagulant effect of heparin in test systems composed of purified factors [antithrombin III (AT-III), fibrinogen and thrombin] was studied. As expected, the sensitivity to heparin depended on the concentrations of AT-III and thrombin, whereas the fibrinogen level was less decisive. In addition, qualitative differences proved important. Thus, the sensitivity to heparin was greater with crude bovine thrombin than with highly purified thrombin from the same species. Further, the sensitivity to heparin increased following removal of cold-insoluble material from the fibrinogen preparation. Finally, during storage of purified AT-III at + 4 °C for more than 4 weeks, the sensitivity to heparin decreased more rapidly than expected from the amidolytic AT-III assays. Smaller amounts of heparm were required to give a prolonged thrombin clotting time than in whole plasma, indicating that components of normal platelet-poor plasma (besides AT-III and fibrinogen) interfere with the anticoagulant effect of heparin. The present test systems may prove suitable for the detection and evaluation of factors of importance for the so-called heparin tolerance

ISSN:1424-8832    

DOI:10.1159/000214199

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

The effect on the heparin thrombin clotting time (HTCT) of a so-called acute phase protein, α1-acid glycoprotein, was studied in test systems consisting of purified fibrinogen, antithrombin III, heparin and thrombin, and compared to the effect of platelet material (crude platelet factor 4). In these test systems a physiological concentration of α1-acid glycoprotein (1 g/l) inhibited heparin more effectively than did soluble platelet material from 200 × 109 platelets/1. The present observations suggest that α1-acid glycoprotein is of great significance for the so-called heparin tolerance, whereas platelet material proved less import

ISSN:1424-8832    

DOI:10.1159/000214200

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

Endotoxin-stimulated monocytes develop a very high tissue-factor-like activity as compared to an equivalent number of monocytes in a preparation of monocyte-enriched mononuclear blood cells obtained by Ficoll®-Urovison® density gradient centrifugation which is not exposed to endotoxin. In monocytes which were obtained by plastic adherence from the monocyteenriched mononuclear blood cells, the tissue factor activity was as high as in the endotoxin-stimulated monocyte

ISSN:1424-8832    

DOI:10.1159/000214201

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

The fibrinolytic activity of euglobulin fractions prepared from human morning plasma and assayed on fibrin plates is strongly inhibited by the C1-inactivator present in the fractions. Flufenamate, a potent representative of the group of synthetic thrombolytic agents, eliminates this inhibition in euglobulin fractions. This elimination is an apparently irreversible reaction dependent on concentration, time and temperature. The fibrinolytic enhancing effect of flufenamate in euglobulin fractions correlated well with a similar effect of added C1s, which neutralized the C1-inactivator. The effect of flufenamate was slightly greater than that of added C1s, suggesting an additional effect of the flufenamate. The activity enhancing effect of the flufenamate at the lower molarities could be separated from an activity decreasing effect at the higher molarities. A simple technique by which inhibitory effects in euglobulin fibrinolysis are selectively eliminated is described.

ISSN:1424-8832    

DOI:10.1159/000214202

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

The influence of heparin and heparinoids on the release of plasminogen activator was studied in the isolated perfused pig ear. Heparin enhances the release of the tissue activator of fibrinolysis in a dose-dependent manner within the range of 0.05–1.0 IU/ml perfusion fluid. The release is also caused by sulfated polyanions at concentration of 10 μg/

ISSN:1424-8832    

DOI:10.1159/000214203

出版商:S. Karger AG    

年代:1977

数据来源: Karger

ISSN:1424-8832    

DOI:10.1159/000214204

出版商:S. Karger AG    

年代:1977

数据来源: Karger

ISSN:1424-8832    

DOI:10.1159/000214205

出版商:S. Karger AG    

年代:1977

数据来源: Karger

ISSN:1424-8832    

DOI:10.1159/000214198

出版商:S. Karger AG    

年代:1977

数据来源: Karger