摘要:

When isolated rat livers were perfused with platelet-free erythrocytes suspended in Tyrode’s solution containing 6% bovine serum albumin, the generation of factors V, XI, and XII was clearly demonstrable. On average, after 5 h of perfusion of a single liver, the concentrations in the perfusate (as a percentage of normal rat plasma) were about 6% for factor V, 8% for factor XI, and 5% for factor XII, compared with 20% for factor VII, which was used as the reference standard. When two livers were perfused, approximately twice these concentrations were achieved. When the properties of these factors in perfusate and plasma were compared, they agreed well except for differences in the celite adsorbability of factors XI and XI

ISSN:1424-8832    

DOI:10.1159/000214182

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

Fibrin monomers were made from dissolved clots according to three different methods [(1) method of DOnelly et al., using 1 m NaBr pH 5.2 as clot solvent; (2) method of Monelly-Berghaus et al., using 0.0167 m acetic acid to dissolve EDTA-clots; (3) method of Yudelman et al. using 3.3 m urea pH 7.4 as clot solvent]. The monomers were characterized by agarose gel chromatography, SDS-polyacrylamide gel electrophoresis (with and without reduction with 2-mercaptoethanol) and by acid polyacrylamide gel electrophoresis after sulfitolysis. Clottability and polymerization speed were compared to those of fibrinogen exposed to large amounts of thrombin (‘control monomers’). All preparations contained fibrin aggregates. These were of two different types, either non-covalently linked or covalently but not FSF-cross-linked aggregates. The latter type was probably due to -S-S- bonds, since they could be prevented by iodoacetamide. Methods 1 and 2 produced monomers which did not clot at all in buffer but clotted slowly in serum. Monomers prepared according to method 3 clotted even faster than did fibrinogen exposed to large amounts of thrombin. This was apparently due to the content of substantial amounts of covalently linked fibrin aggregates. When these were prevented by adding iodoacetamide under preparation, the monomers so produced had lower clotting speed than ‘control monomers’. From the present investigation it must be concluded that extrapolation of data obtained with fibrin monomers prepared from fibrin clots should be interpreted with gre

ISSN:1424-8832    

DOI:10.1159/000214183

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

Fibrin Monomers were prepared by exposing human fibrinogen to thrombin in the presence of 3.3 m urea, pH 7.4 and +20 °C. Both with thrombin covalently bound to Sepharose 4 B and with thrombin in solution (100 NIH units/ml), extensive conversion of fibrinogen to fibrin within 1 h was achieved. No spontaneous clotting occurred.The fibrin preparations consisted to a very large extent of fibrin monomers, as evidenced by N-terminal analysis, agarose gel chromatography and SDS-polyacrylamide gel electrophoresis. When the urea concentration was reduced by dilution, the fibrin monomers retained their normal clottability, polymerization speed and cross-linking ability. Identical results were obtained with radioactive fibrin monomers prepared from 125I-labelled fibrinogen. Storage for 4 weeks at +4 °C or quick freezing in liquid nitrogen and subsequent thawing caused only slightly prolonged polymerization times. Attempts to separate thrombin in solution from fibrin monomers by chromatography were unsuccessful, whereas this was easily achieved with insolubilized thrombin. Since traces of thrombin may influence the experimental results, the latter technique should be preferre

ISSN:1424-8832    

DOI:10.1159/000214184

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

Two peaks were demonstrated after gel chromatography on Sepharose 6-B of noncovalently bonded fibrin dissolved in 1 M NaBr at pH 5.3. One was eluted at the void volume while the other one was eluted at the elution volume of fibrinogen. Both fractions were stable during rechromatography. The fraction eluted at the void volume was highly heterogeneous and consisted of noncovalently bonded aggregates of fibrin monomer units. Our experiments indicate that these high molecular weight derivatives develop during dissolution of fibrin in NaBr solution. The possible explanation of these results is discussed.

ISSN:1424-8832    

DOI:10.1159/000214185

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

The generalized Shwartzman reaction was produced in rabbits by two intravenous injections of endotoxin given 20–22 h apart. Prior to the second injection of endotoxin, the animals were made hypovolemic by removal of 40% blood volume or reduction of the systolic pressure to 70% of normal, whichever came first. This blood removal produced a persistent hypotensive episode. 8/9 rabbits made hypotensive in this manner had evidence of glomerular capillary thrombi compared to 8/8 in a group that did not have blood removed. It is concluded that hypovolemic shock of this magnitude does not prevent the generalized Shwartzman reactio

ISSN:1424-8832    

DOI:10.1159/000214186

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

56 patients undergoing elective major surgery received low dose heparin prophylaxis, 5,000 IU 2 h before surgery and every 8 h for 7 days. The patients were tested by the 125I-fibrinogen uptake test, which, if positive, was controlled by venography. The heparin concentrations were measured by the method of Denson and Bonnar and by the chromogenic method of Teien et al. in blood samples drained immediately before and after surgery and on the 1st and 5th postoperative day 2 and 4 h after the injection of heparin. The values were found higher when estimated by the method of Denson and Bonnar than by the chromogenic method. Deep vein thrombosis (DVT) developed in 11 patients, and in 10 of them within the first 48 after the operative procedure, in 1 after 4 days. The preoperative values were not lower in the patients who developed DVT, and the heparin concentrations found on the 1st and 5th postoperative day were at the same level as in those who did not develop DVT.

ISSN:1424-8832    

DOI:10.1159/000214187

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

Ditazole (4,5-diphenyl-2-bis-(2-hydroxyethyl)-aminoxazol) inhibits in vivo platelet aggregation induced in mice by intravenous injection of cells derived from an experimental tumor, the Lewis lung carcinoma. Such a protective effect of ditazole could not be observed when the number of circulating platelets dropped slowly following intramuscular implantation and spontaneous dissemination of the same cancer cells. These results support previous observations suggesting a different mechanism for the thrombocytopenia observed after intravenous and intramuscular injection of cancer cells. Tail transection bleeding time of normal mice is significantly prolonged by ditazole, a finding at variance with that reported in rats.

ISSN:1424-8832    

DOI:10.1159/000214188

出版商:S. Karger AG    

年代:1977

数据来源: Karger

摘要:

In a double-blind cross-over study the influence of acetylsalicylic acid (ASA) and paracetamol on menstrual blood loss has been investigated in 23 women without an intrauterine loop or birth control pills. After intake of ASA there was no significantly, increased blood loss compared with intake of placebo or paracetamol.

ISSN:1424-8832    

DOI:10.1159/000214189

出版商:S. Karger AG    

年代:1977

数据来源: Karger