ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01145.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Macrophage ferritin content was determined following culture of peripheral blood monocytes for a period of 8 d in 40% autologous plasma to render them mature macrophages. Ferritin content was measured prior to and following culture using the radioimmunoassay. The normal range of values was established in a group of 22 healthy volunteer blood donors. A significant increase in the ratio of macrophage/ monocyte ferritin was observed in every donor studied (range 1.2 ‐ 1.8, p<0.001). Also, a further significant increase was observed when these macrophages were additionally incubated for 6 h with heterologous antibody‐coated sheep red blood cells (range 1.2 ‐ 1.57, p<0.001). Finally, the same studies were performed on a group of thalassemic patients with and without intrinsic iron overload. Again there were significant increases in monocyte ferritin content following culture as well as ingestion of heterologous sheep red cells, with magnitudes similar to those obtained with normal donor monocytes. Therefore we could not demonstrate the presence of a cellular ferritin synthesis defect in macrophages of thalassemic patients with intrinsic iron overload to explain the uncontrolled absorption of dietary iron from thei

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01146.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

15 cases of acute leukaemia (AL) displaying a TdT +, HLA‐DR + phenotype were studied; surface immunoglobulins, T cell markers and the common acute lymphoblas‐tic leukaemia (c‐ALL) antigen were negative, as were peroxidase and non‐specific esterase cytochemical reactions. All cases were extensively investigated by conventional immunofluorescence (IF) and immunoperoxidase (IP), with a panel of monoclonal antibodies (MoAb), using both light and electron microscopy, and for ultrastructural myeloperoxidase (MPO). 8 cases, which were OKB2+, BA1 +, B4+, J5‐ and BA2‐by IF, expressed the J5 antigen in IP. These cases were therefore re‐classified as ALL with a weak expression of the C‐ALL antigen. The other 7 cases showed an OKB2‐, BA1‐, B4+, BA2+ phenotype at IF and were also positive for 1 or more anti‐myeloid MoAb. These features were confirmed by IP study. 4 patients also presented ultra‐structural positivity to MPO. These cases were considered as proliferations of early precursor cells capable of expressing both myeloid and lymphoid features. This study, while demonstrating the heterogeneity of TdT +, HLA‐DR + AL, suggests that the cell origin of many cases may be defined by extensive immunotyping at both IF and IP level. The prognostic and therapeutic implications of these findings are discussed, also in view of the poor prognosis often observed in the more und

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01147.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Migratory properties of malignant lymphocytes in 27 patients with chronic lymphocytic leukaemia (CLL) were investigated using 3‐D collagen gel. There was significant impairment of migration in malignant lymphocytes as compared with normal lymphocytes. Moreover there was an inverse correlation between Rai's staging and the migration index. Patients with progression of disease also showed a decrease in migration index. There was no correlation between kappa and lambda type in terms of migration index. The prognostic significance of the migratory properties of malignant lymphocytes is also discusse

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01148.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The survival of 137 consecutive chronic lymphocytic leukemia patients, diagnosed between 1960 and 1982 and followed up at the Hematology Clinic of the Chaim Sheba Center, was correlated with demographic, clinical and laboratory data. The median survival time of the whole group was 104 months. Older age, hepatomegaly, anemia, thrombocytopenia and increased percent of lymphocytes in the peripheral blood at diagnosis were all associated with shorter survival. On the other hand, splenomegaly or lymph node enlargement did not influence survival. When divided according to both Rai's and the International Workshop staging systems, the survival of our patients seemed to be better than that reported in most previously published series of similar patients. We assume that this is related to a conservative approach to the treatment of chronic lymphocytic leukemia patients in this center.

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01149.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Patient records from January 1975 to December 1984 were analysed to assess the possible incidence of protein‐bound vitamin B12malabsorption. This condition is characterised by a low serum vitamin B12level and a normal Schilling test but impaired absorption of vitamin B12bound to protein. We found that 48 (25%) patients with a low serum cobalamin level unexplained by other causes had a normal Schilling test. Megaloblastic haemopoiesis was found in 25 of these. From this group, all 10 patients who had a test of protein‐bound vitamin B12absorption showed impaired absorption. Protein‐bound vitamin B12malabsorption may represent an early phase of pernicious anaemia when hypochlorhydria precedes intrinsic factor deficiency and should be tested for when the serum vitamin B12level is decreased and the Schilling test is n

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01150.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The effect of hydrocortisone on the formation of erythroid colonies was studied in vitro in cultures of normal human bone marrow in an agar system. A range of concentrations of hydrocortisone (10‐10to 10‐3mol/1) showed significant effects on erythroid burst formation, in terms of the number of colonies, on d 10 and 14 of culture. At subphysiological concentrations (10‐10to 10‐8mol/1), no effect was seen, but at both physiological (10‐7mol/1) and pharmacological (10‐6and 10‐5mol/1) concentrations stimulation of erythroid burst formation was noted. At 10‐4mol/1 hydrocortisone inhibited erythroid colony formation and 10‐3mol/1 was uniformly lethal. In the concentration range of 10‐7to 10‐5mol/1 hydrocortisone also appeared to increase erythroid colony size. Thus hydrocortisone (10‐7to 10‐5mol/1) stimulates erythroid colony growth and it is suggested that the hormone may play a role in the physiological regulatio

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01151.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Low serum cobalamin levels in 10 patients with AIDS or AIDS‐related complex led us to also prospectively survey 40 homosexual men in our AIDS clinic. 8 of the latter (20%) had low cobalamin values. We found no evidence of megaloblastic changes in the blood or bone marrow. Assessment disclosed malabsorption of cobalamin in only 1 of 6 cases tested for it. 6 of the patients were treated with cobalamin and had no hematologic response. It appears that low serum cobalamin levels in AIDS and related disorders do not usually represent overt cobalamin deficiency. While malabsorption is occasionally responsible for the low cobalamin level, in most cases the cause is unknown and may reflect a serum abnormality similar to that in multiple myeloma. AIDS and related disorders should be considered in the differential diagnosis of unexplained low cobalamin level

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01152.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01153.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

92 patients with acute myeloid leukemia were classified according to the FAB classification (M1 n = 20, M2 n = 43, M3 n = 1, M4 n = 19, M5a n = 2, M5b n = 2, and M6 n = 5 patients). Serum measurements of lactoferrin (LF), myeloperoxidase (MPO) and lysozyme (LYS) were performed before the start of treatment. LF was significantly lower in M1 when compared with M2 but not as compared to M4, MPO was significantly higher in M2 and M4 than in M1, but comparable MPO levels were found in M2 and M4. LYS was significantly elevated in M2 in comparison with M1, and in M4 when compared to both M1 and M2. Polymorphonuclear granulocytes (PMNs) in M1 were significantly reduced when compared with M2 and M4, whereas mononuclear cells were significantly increased in M4 in comparison with both M1 and M2. FAB classification did not generate any prognostic information. When the patients were, instead, subdivided according to LF levels we found prognostically significant differences. Of patients below 100 μg/1, 44% went into remission as compared to 77% with LF from 101 to 400 μg/1. In patients with LF levels above 400 μg/1 the remission frequency was only 14%. Multivariate statistical analysis on the data further suggested that lactoferrin may be used as an independent prognostic indicator. We conclude that although determination of the serum‐levels of lactoferrin, lysozyme and myeloperoxidase in certain cases may be valuable as a supplement to the morphological examination of acute myeloid leukemia, it is evident that none of the three determinations can be used alone to distinguish between the FAB groups. However, we suggest that lactoferrin may be used as a prognostic indicator and may even be used to recognize a new variant of AML with a particularly poor prognosis ‐high lactoferr

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb01154.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY