ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb00001.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

An unusual case of ‘pseudo‐lymphoid’ leukaemia is described. The leukaemic cells resembled small, mature lymphocytes but lacked B‐ and T‐cell membrane markers as well as immunoglobulin (Ig) and T‐cell receptor gene rearrangements. They showed, instead, features of early myeloid cells since they expressed 2 myeloid antigens, CDW13 and My9, and displayed peroxidase activity demonstrable by electron microscopy (EM) on unfixed cells. Cytogenetic studies showed monosomy 5, t(4;17) (p12;p11), t(2;3)(p23;q14) and an abnormal chromosome 12. Abnormalities of chromosomes 4 and 5 have been previously associated with ‘pseudo‐lymphoid’ leukaemias. This case illustrates the value of sensitive methods for the characterization of blast cells and for the precise diagnosis of leukaemias with apparent ‘l

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb00002.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

It has been recently demonstrated that ecto‐5′nucleotidase (5′NT) activity is significantly decreased in the peripheral blood lymphocytes (PBL) of homosexual men. This paper reports a study of PBL 5′NT activity in 38 hemophiliacs at risk for the acquired immunodeficiency syndrome (AIDS). The enzyme activity was correlated to the immunologic and virologic data. T‐cell subset distribution was unbalanced and directly correlated with the cumulative amount of AHF infused. PBL 5′NT activity, however, was similar to that of healthy controls. 6 patients displayed serum antibodies to the human immunodeficiency virus (HIV) but no decrease in PBL 5′NT activity. In conclusion, these data indicate that both heavily treated and seropositive as well as untreated hemophiliacs have normal PBL 5′NT activity. This striking dissimilarity between homosexual men and hemophiliacs suggests that some immunologic alterations leading to 5′NT deficiency occur i

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb00003.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Analyses on the prognostic impact of cellular markers in chronic lymphocytic leukaemia is hampered by the strong influence of age on survival. We have therefore used a ratio between the observed survival (or observation time) and the expected survival of age‐ and sex‐matched healthy Swedes to eliminate the age dependence. Log rank analysis of the observed/expected survival time ratio demonstrated that CLL‐patients with trisomy 12 had a poorer age‐corrected survival than other p

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb00004.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Inhibitory activity in extract from human blood granulocytes was tested on gran‐ulocyte‐macrophage colony formation in vitro. The inhibition depended on the type of serum used. With mouse BMC and FCS in the cultures, extract corresponding to 2.5 times 104granulocytes/ml reduced the colony number by 35%, and extract from 2 times 105cells caused maximal inhibition (80–90%). With HS and mouse BMC the colony number was reduced by only 11–12%, but stronger inhibition (55%) was observed when the serum concentration was reduced. With both types of sera the total cell number per culture plate was reduced relatively more than the colony number. Human GM‐CFC were as sensitive as mouse GM‐CFC, and extract from CML granulocytes inhibited less (p<0.01) than extract from normal cells. Biochemical studies indicated that the inhibitor is a protein with a molecular weight of 30–60000. Lactoferrin, a putative inhibitor of CSF production, did not inhibit spontaneous or CSF‐induced colony formation i

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb00005.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Reduced (GSH) and oxidized (GSSG) glutathione levels in arterial and venous rabbit red blood cells (RBC) and plasma are reported. No significant differences were noted between arterial and venous erythrocytes GSH and GSSG content. Plasma GSH levels were found to be the same in both arterial and venous samples. Contrarily, the concentrations of GSSG were higher in venous than in arterial plasma. These findings cannot be attributed solely to the increased RBC oxidative activity that occurs in the venous system. The phenomenon may reflect the oxidative state present in other tissues and may thus be a harbinger of differences in GSH content.

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb00006.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The serum thymidine kinase (S‐TK) and proliferative activity of the leukemic cells were determined in 27 untreated patients with chronic lymphocytic leukemia (CLL). A significant positive correlation between S‐TK and proliferation expressed as a proliferative index (PI) was found (r = 0.70, p<0.001). Additionally, PI (r = 0.59, p<0.01) and S‐TK (r = 0.47, p<0.05) correlated to peripheral blood lymphocyte count. When different variables and combinations of variables were studied in order to define their capacity for discriminating between progressive and indolent CLL, S‐TK activity and PI proved to be powerful indications. In longitudinal studies, both S‐TK and PI paralleled disease activity. A model where a combination of S‐TK and PI gives information of the degree of localized disease

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb00007.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The clinical and haematological findings in 131 patients with myelodysplastic syndromes (MDS), none of which had previously received chemotherapy or radiotherapy, classified according to the FAB criteria, were analysed. The distribution among the 5 subgroups was: RA 31 patients, RAS 19, RAEB 23, CMML 29 and RAEBT 29 patients. There were difficulties in the classification of 24 patients. These included, first, 8 cases with myeloid hyperplasia of the bone marrow (BM) but without mono‐cytosis or excess of blasts of the BM. They were classified as RA. Second, 8 cases with sideroblastosis but with monocytosis or excess of blasts of the BM were classified 3 as RAEB, 2 as CMML and 3 as RAEBT. Finally, 8 cases with absolute monocytosis and BM blasts 15–30% were classified as CMML. 37 of 82 dead patients (45.1%) had transformed to acute non‐lymphoblastic leukaemia (ANLL). The incidence of evolution to ANLL was low for RA and RAS (6.30% and 12.5% respectively), while it was 37.5% for RAEB, 57.1% for CMML and 77.2% for RAEBT. The median survival for each subgroup was: RA 18 months; RAS 25; RAEB 13; CMML 14 and RAEBT 10 months. It is concluded that the FAB classification with some modifications recognises group of MDS with different prog

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb00008.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Ultrastructural features thought to be significant in acute myeloid leukaemia (AML) have been examined in 100 cases entered into the Medical Research Council 9th AML Trial. It is concluded that, of the features examined, only rarely is there a striking correlation with diagnosis which could be usefully employed with confidence. This is a consequence of there being both a large overlap of similar ultrastructural features between the different sub‐types of the disease and also wide variations in ultrastructure within the conventionally diagnosed sub‐types of AML. Thus the similarities between cells from different cases which allow them to be classified by light microscopy do not necessarily extend to the ultrastructural level. However, it is suggested that a classification on ultrastructural grounds might have a prognostic significance which would only be revealed by a long‐term

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb00009.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

To elucidate whether a relationship existed between bone marrow donor cytomegalovirus (CMV) immune status and the probability of staying in remission after transplantation, a retrospective multicentre analysis was performed in 69 patients who received allogeneic bone marrow transplantation during relapse or second remission of AML or second remission of ALL. None of 12 AML patients with CMV seropositive donors had posttransplant relapse, in contrast to 7 of 10 AML patients with seronega‐tive donors. Kaplan‐Meier estimates of the 2‐yr probability of staying in remission for the two groups were 100% and 0%, respectively (p<0.0005). This effect was independent of disease stage, donor and recipient age, recipient pretransplant CMV immune status and the occurrence of posttransplant CMV infection in recipients, and was not mediated through an increased occurrence of overt graft‐versus‐host disease (GvHD) in recipients with CMV seropositive donors. The increased probability of staying in remission was associated with an increased probability of 3‐yr disease‐free survival (p<0.01). No similar effect was observed in patients with ALL. This study may suggest an allograft‐versus‐leukaemia effect in AML, associated with CMV seropositive donors, which seems separate from GvHD and independent of the occurrence of posttranspl

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1987.tb00010.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY