摘要:

Megakaryocyte colony formation, as identified by conventional techniques, was observed in precursor cell cultures from peripheral blood in 8 of 20 consecutive patients with diagnosis of myeloproliferalive disease (4/11 patients with polycythemia vera, 3/5 with essential thrombocythemia, 1/2 with primary osteomyelofibrosis and 2 with a myeloproliferative syndrome not further assessable), but not in 50 healthy controls (p<0.0001). 7 cultures showed spontaneous erythroid colonies, but were negative for megakaryocyte colonies. Megakaryocyte colony formation was independent of added erythropoietin, plasma or human leukocyte‐conditioned medium, but was dependent on the presence of accessory cells. The cells in megakaryocyte colonies had the characteristic morphology of megakaryocytes and stained positively with the IIIa/IIb monoclonal anti‐platelet antibody. Thus, megakaryocyte colony formation by precursor cells from peripheral blood in the absence of exogenous stimulating factors seems to be a phenomenon specific for myeloproliferative disease. Differential diagnosis of thrombocythemia may be facilitated by demonstration of endogenous megakaryocyte colony formation, which does not occur in secondary dise

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb01219.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Erythroid colony formation in vitro was studied in 80 patients with erythrocytosis. 43 of the patients had polycythaemia vera (PV), 18 patients had secondary erythrocytosis, 6 had normal red cell mass, and 13 patients were regarded as unclassified. Spontaneous erythroid colony formation, in the absence of exogenous erythropoietin in the cultures, was discovered in all patients with PV, whereas no patient with secondary erythrocytosis or with normal red cell mass showed this phenomenon. 8 of the 13 patients with unclassified erythrocytosis spontaneously formed erythroid colonies. 7 patient with unclassified erythrocytosis have been followed for 5 yr. 3 of the 4 patients with spontaneous colony growth but none of the 3 without it can now be classified as PV. Thus, spontaneous erythroid colony formation indicates PV even in early and atypical cases. Therefore, the culture of erythroid progenitors is very useful in the differential diagnosis of problematic cases with erythrocytosis.

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb01220.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

A consecutive material of 111 untreated patients with non‐Hodgkin's lymphoma was studied with respect to fraction of S‐phase cells in blood mononuclear cells in relation to presence of monoclonal B cells in blood (MBCB). Fraction of S‐phase cells was determined by flow cytometry and estimation of MBCB was performed by kap‐pa:lambda analysis. The fraction of S‐phase cells was significantly higher (p<0.001) in MBCB‐positive cases (median 1.2%) than in the MBCB‐negative (median 0.7%). MBCB‐positive patients with S‐phase values ≥ 1.5% had a less favourable prognosis compared to those with<1.5% cells in S‐phase (p = 0.01). In a Cox multiparameter analysis, advanced clinical stage, high‐grade morphology and high fraction of S‐phase cells in blood in MBCB‐positive cases were independent, statistically significant, negative prognostic indicators. The results indicate that an elevated S‐phase value in blood in non‐Hodgkin's lymphoma constitutes a negative prognostic factor, probably reflecting prol

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb01221.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

We report our experience of peripheral blood and bone marrow changes in patients with HIV disease. Abnormalities were most commonly seen in patients with advanced disease. In AIDS group IV patients (CDC classification) anaemia (92%) neutropenia (85%) monocytopenia (75%) and thrombocytopenia (61%) have their highest incidence, the reason being a combination of factors such as infection, myelosuppressive drugs and HIV infection itself. Bone marrow examinations were performed most commonly for microbiological culture (25%) and the investigation of anaemia (16%). Morphological changes in the bone marrow were non‐specific and not pathognomic; however erythroid hypoplasia was found to be a distinctive feature associated with MAI infection. The procedure provided a high yield for microbiological culture, particularly in MAI infectio

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb01222.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Gallium‐67 scanning was performed pre‐ and post‐therapy in 25 patients with Hodgkin's disease and a mediastinal mass. At restaging after therapy, radiographs (or CT scans) did not predict the presence of active disease whereas gallium scans did with a high degree of accuracy. Gallium‐67 determined disease activity in those patients who had a residual mediastinal mass predicting outcome in 11 out of 12 patients; one had a late relapse at 7 years. In patients without a residual mass gallium scanning was again accurate, predicting outcome in 11 of 13 patients. Two patients with negative gallium scans but subsequent active disease were scanned too soon after chemotherapy. The results suggest that gallium scanning has an important role in the mangement of mediastinal Hodgkin's disease and is superior to all current methods of assessing disease activity irrespective of the presence of a residual mediastin

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb01223.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

To evaluate the effects of recombinant G‐CSF and GM‐CSF on RAEB and RAEB‐T cells, blast cells from 6 patients were incubated in liquid culture systems with these CSFs for 7 days, and their numerical, morphological and functional changes were assessed. Both CSFs stimulated cell growth, but decreased the proportion of blast cells in 5 of the 6 cases. Karyotypic abnormalities persisted during cultivation in some cases. The CSFs also stimualted the expression of part of the esterase activities, and a positive interaction of both CSFs was seen in part. Although CSFs had no significant effects on the ability of cells to reduce NBT or to phagocytize latex particles, the results indicated that they induce partial differentiation of blast cells. It appears that such pathological cells still retain the capacity to respond to growth fa

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb01224.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

In 18 β‐thalassaemia families from the Ferrara area the incidence of an inherited low flavin mononucleotide (FMN)‐dependent pyridoxine phosphate (PNP) oxidase activity, a sensitive indicator of red‐cell FMN deficiency, is higher in related members in these families than in the unrelated spouses and controls subjects without family history of thalassaemia. This suggests slower red‐cell riboflavin metabolism in thalassaemia families, which may have resulted from selection in combination with thalassaemia by malaria. However, there was a markedly higher incidence of red‐cell flavin adenine dinucleotide (FAD) deficiency in thalassaemia heterozygotes than in their normal relatives. This was indicated by higher stimulation of FAD‐dependent glutathione reductase (GR) activity by FAD and lower GR activity per red cell, and suggests a marked additive effect by thalassaemia on the red cell FAD deficiency that results from the inherited slow riboflavin metabolism. There is evidence that diversion of FAD to other FAD‐dependent enzymes might be an im

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb01225.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Persistent elevation of lymphocyte counts is usually associated with a malignant monoclonal lymphoproliferative disease. Over the last 8 years, amongst patients investigated in our center for undetermined persistent lymphocytosis, a diagnosis of malignant lymphoproliferation was excluded in 6 cases as studies of surface membrane immunoglobulin light chains showed that they presented a polyclonal expansion of their B‐lymphocyte pool. All patients were young‐to‐middle aged women presenting peculiar immunohematologic findings characterized by 1) persistent (2–7 yr) elevation of lymphocyte counts (4–14 × 109/l), 2) presence of characteristic binucleated B cells on peripheral blood smears, 3) a normal bone marrow histology, 4) a polyclonal increase of serum IgM with low‐to‐normal IgG and IgA levels. Histologic examination of the spleen in 2 patients and lymph nodes in 1 showed a benign follicular lymphoid hyperplasia. The evolution was benign in every case. We suggest that chronic polyclonal B‐cell lymphocytosis is a distinct clinicopathologic entity that should not be confused with malignant lymphoprolife

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb01226.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Peripheral T‐cell lymphomas (PTL) are morphologically and immunophenotypically heterogeneous. We have examined a series of cases to determine whether this heterogeneity is reflected at the level of developmentally specific T‐cell receptor (TCR) gene rearrangement. 4 of 5 cases had clonal rearrangements of TCR beta and gamma chain genes; one of these also had a probable DQ52‐J immunoglobulin heavy chain gene rearrangement. 2 of the 4 TCR gamma gene rearrangements involved the most 5′ J region, JP1, a characteristic of immature thymocytes. These 2 cases also had immunophenotypic features of immaturity. Taken together, our results suggest that TCR gene rearrangement is correlated with surface marker data and shows that in some cases PTL may arise from a very early stage of thymocyte mat

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb01227.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The effect of recombinant murine interleukin‐3 (rIL‐3) and recombinant murine granulocyte‐macrophage colony‐stimulating factor (rGM‐CSF) on in vitro murine myeloid progenitor cell (CFU‐C) growth and on the function of murine resident peritoneal macrophages was investigated. both rIL‐3 and rGM‐CSF are known to support the growth of CFU‐C and, when combined, were found to act synergistically to induce the development of an increased number of CFU‐C. The distribution pattern of myeloid colonies in the presence of these two growth factors was in general similar to that in the presence of rGM‐CSF alone. both rGM‐CSF and rIL‐3 enhanced the phagocytosis ofCandida albicans(CA) by mature macrophages producing an increase in the percentage of phagocytosing cells as well as an increase in the number of yeast particles ingested per cell. No additive effect on the phagocytosis was observed when the two growth factors were added concurrently. rGM‐CSF, but not rIL‐3, enhanced the killing of CA by macrophages. This killing was inhibited by

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb01228.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY