摘要:

Abstract:Platelet function and the clinical course of the disease were prospectively investigated in 29 patients with myeloproliferative disorders. Serial determinations (median: 5 investigations per patient within 17 months) of platelet aggregation, plasma and intraplatelet concentrations of β‐thromboglobulin (βTG) and platelet factor 4 (PF4), and of fibrino‐peptide A (FPA) plasma levels were carried out. In the chronic phase of polycythaemia vera, patients with thrombohaemorrhagic complications during the study period had higher platelet count, more severe platelet aggregation defects, and increased plasma levels of βTG and FPA compared to patients without complications. However, thrombohaemorrhagic complications were not predicted by changes in these parameters in the individual patient during the chronic disease phase. When patients with chronic myelogenous leukaemia entered blast crisis, bleeding complications were related to thrombocytopenia, impaired platelet function and low intraplatelet concentrations of βTG and PF4. Cytoreduction by chemotherapy in the chronic phase of CML did not alter βTG and PF4 plasma levels, whereas treatment of polycythaemia rubra vera by venesection favourably influenced platelet α‐granule secretion and increased intraplatelet concentrations of

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1990.tb00456.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:Prognostic factors have been tested in patients with multiple myeloma treated according to a randomized trial of standard therapy versus 5‐drug combination therapy. The following population‐based study included 92 patients with a median age of 70 yr. The median survival was 31 months. The Cox regression model was used to search for predictors of survival. The cut‐off levels for blood analyses derived in earlier studies tended to select few patients in the high‐risk groups, for example only 8% of the patients had hemoglobin (Hb) ≤ 7.5 g/dl. Lytic bone lesions in the pelvis or in the long bones, or spontaneous fractures and age>70 yr gave prognostic information in addition to anemia and impaired renal function. The MRC staging system was a better prognostic tool than the Durie&Salmon stages. Palliative treatment regimens which take quality of life into account should be considered carefully in multiple myeloma patie

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1990.tb00457.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:A study of bone marrow stromal elements in murine acute myeloid leukemia (AML) was carried out. Our previous studies had indicated marrow stromal deficiency in murine AML. In the current investigation, separate stromal cells were cultured and the results obtained have shown that, while marrow stromal macrophages are normal in leukemia and express adequate amounts of IL‐1, the fibroblasts are markedly reduced. However, if sufficient fibroblasts are pooledin vitro, they produce adequate amounts of CSF. Test of TNFα in leukemic cells CM, as possible cause of marrow stromal inhibition in leukemia, had not disclosed this cytokine. Further, it was observed that total body lethal irradiation of leukemic mice aggravates the stromal deficiency, confirming results of our previous investigations. It is concluded that bone marrow stromal deficiency in murine AML is due to decreased fibroblasts and, implicitly, reduced CSF producti

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1990.tb00458.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:We report 6 patients with myelodysplastic syndrome, all of whom showed a bizarre nuclear anomaly within the neutrophils that was characterized by extensive clumping of chromatin into large blocks separated by clear zones, generally associated with a lack of segmentation. Anaemia, thrombocytopenia, variable leucocyte counts with leucoerythroblastic picture, marrow hypercellularity with granulocytic hyperplasia and moderate dysplastic changes in erythroblastic and megakaryocytic lines were present at diagnosis. 2 patients had normal karyotypes and a 3 showed a deletion of chromosome 14. 5 out of 6 patients had pneumonia at diagnosis. The median survival was short (5 months) and haemorrhagic complications were the cause of death in 4 patients. The clinical features and the evolution of these and other reported cases suggest that the presence of abnormal chromatin clumping in leucocytes might be a clue to a new subtype of myelodysplastic syndrome.

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1990.tb00459.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:13 cases of childhood acute lymphoblastic leukaemia (ALL) were studied combining cell surface marker analysis with immunogenotyping by Southern blot hybridisation with a panel of antigen receptor gene probes. The immunophenotypes were unequivocal: 7 patients had B‐phenotype and 6 patients T‐phenotype ALL. In several patients immunogenotypes were not fully consistent with the respective phenotypes. For example, 2 B‐cell precursor ALL had rearranged TCRβ chain genes and 2 T‐ALL rearrangement of Ig heavy‐chain genes. All cases showed clonal rearrangement or deletions within the TCRδ gene locus. TCRδ gene rearrangements might, therefore, serve as markers of clonality but not of B‐ or T‐lineage in immature lymphoid neoplasms. We conclude that in current diagnostic practice immunogenotyping is a supplement rather than an alternative to immunophenotyping by surface

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1990.tb00460.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Abstract:Anin vitromethod of radiolabelling platelets with111In tropolonate in plasma has been devised enabling imaging and cell kinetic studies to be performed in patients with thrombocytopenia (TP) using autologous, rather than donor, platelets. Platelets from 10 TP patients, with platelet counts ranging from 4–91 times 109/1, were labelled in 50% plasma with111In tropolonate, containing the optimum tropolone concentration of 2 times 10−4mol/l, at platelet concentrations ranging from 0.08‐4.5 times 109/ml resulting in labelling efficiencies (LE) between 51 and 86%. In 4 patients, red cells contaminated the platelets but this was corrected for by measuring the proportion of111In on the platelets prior to injection and in the post‐injection blood samples. Platelet recovery (PR), mean platelet life‐span (MPLS) and platelet production rate (PPR) were calculated and splenic and hepatic images were taken. The results clearly show that useful clinical data can be obtained by this method even in patients with

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1990.tb00461.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1990.tb00462.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1990.tb00463.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1990.tb00464.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Book reviewed in this article:Hematology of infancy and childhood Eds. D.G. Nathan F.A. OskiMolecular control of haemopoiesis. Ciba Foundation Symposium 148.Bone marrow purging and processing: progress in clinical and biological research vol. 333. Eds: S. Gross, A.P. Gee&D.A.Modern trends in human leukemia. VIII. New results in Clinical and Biological Research including pediatric oncology.Hematology and blood transfusion 32. Eds. R. Neth, R.C. Gallo, M.F. Greaves, G. Gaedicke, S. Gohla, K. Mannweiler&J. Ritter.

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1990.tb00465.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY