ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1994.tb01277.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract: Three clinical parameters — average steady‐state haematocrit (ASSH), number of crises per year (Cr/Y), and number of transfusions per year (Tx/Y) — were evaluated in 52 patients with sickle‐cell anaemia in relation to their foetal haemoglobin (HbF) levels. No correlation was observed between HbF and any of these parameters. A comparison of these three clinical parameters and the alpha globin gene status was also made in 28 of these patients. The relationships between (ASSH) or (Cr/Y) and alpha globin gene status were not significantly different (p>0.05) but a significantly different value (p<0.05) was observed between (Tx/Y) and the alpha globin gene status in these patients. It is concluded that, although HbF levels did not affect any of these parameters, alpha thalassaemia deletion significantly reduces the transfusion requirements of these

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1994.tb01278.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract: We report on the T‐cell profile and natural killer (NK) function in 22 hairy cell leukemia patients before and 2 and 6 months after a single 7‐day course of 2‐CdA therapy at a dose of 0.1 mg/kg daily continuous infusion. Before treatment, two groups of patients were identified in respect to NK activity, the first (12/22) had a normal number of T and NK cells and a normal NK activity, the second (10/22) showed a severe reduction of CD3+, CD4+, CD16+, and CD56+cells, together with impaired NK activity. All patients had increased serum levels of the soluble interleukin 2 receptor (sIL‐2R). Along with a high complete remission rate (77%), treatment with 2‐CdA produced a significant reduction (p = 0.001) of total lymphocytes (from 1.59 × 109/1 to 0.72 × 109/1), and particularly of CD4+cells (from 0.68 × 109/1 to 0.23 × 109/1), while CD8+, CD16+and CD56+cells were less affected. Despite the dramatic reduction of lymphocytes which lasted for more than 6 months, 7/10 patients with impaired NK function before starting 2‐CdA therapy normalized the cytotoxic function within 2 months. In conclusion, 2‐CdA produces a severe reduction of lymphocytes in general, and of CD4 lymphocytes in particular, whilst it may produce a significant increase in the proportion of NK cells leading, together with a normalization of the sIL‐2R serum levels, to a rapid restoration of the NK cytotoxic function in the

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1994.tb01279.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract: Seventeen patients with suspected drug‐induced neutropenia were referred to our laboratory for investigation within a 10‐year period. In each case, the suspected drugs were incorporated separately intoin vitrocultures of the patients' bone marrow. The cultures were performed in triplicate, using multiple controls. In 10 of these patients a drug‐induced inhibition of CFU‐C was demonstratedin vitro.Thein vitroculture technique is a valuable investigation in patients with suspected drug‐induced neutropenia, as it can help identify the causative agent, especially in cases of multidrug administration. It also has a useful application in allowing the clinician to predict drugs to which a patient may be unduly sensitive, and prescribe

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1994.tb01280.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract: Monosomy 7, a deletion of the long arm of chromosome 7, was shown in the neutrophils of peripheral blood in 5 of 6 patients with a myelodysplastic syndrome or leukemia who had monosomy 7 in their bone marrow cells. In a chemiluminescence assay the production of reactive oxygen species by neutrophils from patients was increased in whole blood and decreased in purified cells, which suggests that neutrophils with monosomy 7 tolerate poorly the cell purification procedures used. Thein vitromigration of purified neutrophils obtained from patients with monosomy 7 was impaired. It is known that patients with monosomy 7 have an increased susceptibility to infections. It is possible that neutrophils with monosomy 7 are too easily triggered to a full‐scale respiratory burst and thereby the cells exhaust their ability to eliminate invading microbes efficien

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1994.tb01281.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract: Red blood cell (RBC) deformability was determined with an ektacytometer in fractions separated on the basis of differences in cell volume or density. Deformability was measured with ektacytometry (rpm‐scan and osmo‐scan). We studied three groups of RBC fractions: 1. By counterflow centrifugation we obtained fractions of different cell age which showed a slight decrease in mean corpuscular haemoglobin concentration (MCHC) and an increase in surface‐to‐volume (S/V) ratio in fractions with older cells. 2. By Percoll fractionation fractions were obtained which showed a pronounced increase in (MCHC) but no change in S/V ratio. 3. By a combination of both fractionation techniques, fractions were obtained which showed an increased MCHC and an increase in S/V ratio. Deformability in group 1,2 and 3 showed respectively no change, a moderate decrease and a pronounced decrease in fractions of older cells. A decline in deformability occurs during the aging process of the red blood cell. This decline in deformability in old red cells is greater than originally thought. This decline is the result of an increase in haemoglobin concentration and a second factor, probably a decrease in membrane e

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1994.tb01282.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract: A family with congenital dyserythropoietic anaemia type III was studied. Twenty patients and 10 of their healthy siblings were clinically examined and questioned about their medical history. Blood sampling and bone marrow aspirations were also performed. Forty‐five percent of the patients reported symptoms of anaemia and 35% regularly felt weakness, fatigue, or headache. However, the majority of the patients regarded themselves as healthy. The bone marrow showed a uniform picture of erythroid hyperplasia with multinuclear erythroblasts and gigantoblasts with up to 12 nuclei. There was laboratory evidence of intravascular haemolysis and mild anaemia. We also observed a high prevalence of monoclonal gammopathy of undetermined significance (3 cases) and myeloma (1 case) among the patie

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1994.tb01283.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract: This study is designed to investigate whether apoptosis occursin vivoin pediatric patients with acute leukemia during induction therapy. When patients with common acute lymphoblastic leukemia (cALL) and acute myeloblastic leukemia (AML) were treated with prednisolone (60 mg/m2/day, p.o. or i.v.) and etoposide (150 mg/m2/day, i.v.), respectively, the blast cell counts fell to below 30% and 5%, respectively, in 1 week. However, during this cytoreduction phase, neither morphologically apoptotic cells nor fragmentation of DNA derived from peripheral blast cells were detected at any preparations. On the other hand, cALL but not AML cells spontaneously undergo apoptosis following their culturein vitro.The addition of autologous serum instead of fetal calf serum substantially prevented apoptosis from occurring spontaneously in cALL cells. When cALL and AML cells freshly obtained from patients before therapy were treatedin vitrowith 10 μmol/1 prednisolone and 20 μg/ml etoposide, respectively, these cells underwent apoptosis within 6 hours, as determined by a morphological and DNA fragmentation assay. Thesein vivoandin vitrofindings suggest that, although anticancer drugs may induce apoptosisin vivo, these apoptotic cells cannot be detected due to their rapid removal from the circulat

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1994.tb01284.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

摘要:

Abstract: Stem cell factor (SCF), the ligand of the c‐kit receptor, is a potent enhancing cytokine for haematopoietic cells in the presence of IL‐3, GM‐CSF and erythropoietin (Epo). In the clonogenic assays of 63 MDS patients, the addition of rh‐SCF + GM‐CSF and/or IL‐3 induced a significant increase (p<0.001) in the number and size of CFU‐GM. Never reaching the levels of controls, this increase was seen in all FAB subtypes, but particularly in RA. There was no significant increase in cluster formation, even in RAEB or RAEBt. Rh‐SCF (10 ng/ml) led to mean increases of up to 26 times in the number of Epo‐dependent BFU‐E colonies, particularly in RA (p<0.001) and RAEB (p<0.05). Individual responses varied widely (especially in RA) from no response to supranormal levels. Added to the weekly refeed of 37 MDS LTBMC, SCF (10 ng/ml) induced only a 7% mean increase in both cell output and the number of clonogenic cells recovered in the supernatant. Immunohistochemical examination of the supernatant showed significant increases in differentiating myeloid cells in all examined cases, and in erythroid cells in 3 cases; blast cells increased in only 3 cases. These data suggest that rh‐SCF is capable of at least partially reversing defective MDS myeloid haematopoiesis, and leads no overt risk of leukaemic transformation. Its potent effect on erythroid cells is encouraging for future clinical applications in patients, particulary if they are selected b

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1994.tb01285.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1994.tb01286.x

出版商:Blackwell Publishing Ltd    

年代:1994

数据来源: WILEY