ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00103.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Intermediate‐dose (25 mg/m2) intravenous melphalan has been evaluated in 34 multiple myeloma patients refractory to standard chemotherapies. The median time from diagnosis to entering of patients into the study was 27 months (range 7–71 months). A response was obtained in 12/34 patients (35%). 4 of 12 responding patients have relapsed and 2 of these have died; 8 responders have not relapsed and are still alive. The median duration of survival after 28 months of follow‐up has not yet been reached in the group of patients responding to treatment. However, the overall median duration of survival for the 34 patients entered into the study was 8 months. The median duration of response was 16 months. Toxicity was limited to leukopenia, thrombocytopenia, nausea and vomiting. This lack of severe toxicity allowed us to administer the drug on an outpatient basis. The response rate and the low toxicity observed in this group of patients are encouraging and suggest that intermediate‐dose intravenous melphalan is an effective and safe second line treatment for patients with multiple myeloma not responding to conventional tr

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00104.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Monoclonal antibodies 8A and 62B1, recognizing plasma cell‐associated antigens, were covalently linked to saporin 6, a ribosome‐inactivating protein similar to the A‐chain of ricin. Both immunotoxins were tested on target human cell lines U266 and Raji, on non‐target K562 cell line and on myeloid CFU‐GM progenitors. The cloning efficiency and viability of target cells were strongly reduced by 8A‐saporin 6 and 62B1‐saporin 6 immunotoxins, with an ID50up to 200000‐fold lower than free saporin 6, whilst the K562 non‐target cell line was unaffected. Normal human myeloid precursors (CFU‐GM) were inhibited by immunotoxins only to a limited extent. An application of this model for autologous bone marrow transplantation in multiple myeloma patients is proposed. Since no eradication of cloning target cells was achieved by a single immunotoxin, mixtures made with different antibodies could help

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00105.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Chromosome banding studies carried out on bone marrow cells from a 57‐year‐old white caucasian male with an M1 acute non‐lymphocytic leukemia (ANLL) revealed an unbalanced translocation involving chromosomes 1 and 5 [der(5)t(1;5)(q23;q33)] as part of complex abnormalities in 76% of the cells analyzed. This chromosomal abnormality is the first to be reported in an adult patient with acute non‐lymphocytic leukemia. A review of previous reports on translocations involving the juxtaposition of the 1q23 ŕ qter DNA segment to other chromosomes suggests that this new translocation may be specifically involved with abnormal myeloid proli

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00106.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

By means of a scintigraphic method for estimation of spleen size, the relationship was studied between spleen size and anaemia, total blood volume and red cell volume in patients with myeloproliferative disorders. There was significant correlation between spleen size and Hb, RBC and PCV in myelofibrosis. The size of the enlarged spleen did not, however, correlate with the degree of anaemia in the leukaemias. There was positive correlation between spleen size and red cell volume in these conditions. It was not possible to evaluate the severity of polycythaemia from spleen size and the degree of enlargement of the spleen does not appear to be a reliable parameter for staging and deciding on treatment in patients with PPP.

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00107.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The longitudinal follow‐up is described of 36 anti‐HIV positive haemophiliacs who had seroconverted in the period 1980–82, and of 41 seronegative controls. Laboratory variables were followed up for a mean duration of 2.5 years (1985–87). Of the 36 seropositive patients, AIDS developed in 3, and generalised persistent lymphadenopathy in 9. The HIV‐seropositive patient group had lower CD4:CD8 ratios and CD4 counts but higher CD8 counts than the seronegative group. However, there was no deterioration in the values for the lymphocyte subsets during follow‐up. Titration on paired sera showed an increase in anti‐HIV titre with time. Testing for the presence of HIV antigen was positive in 5 patients, including 2 who later developed AIDS. We conclude that anti‐HIV positive haemophiliacs, though actively immunised, often show no symptoms even as long as 7 yr after seroconversion and that, in certain patients, the immune system may even show signs

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00108.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

From 1973 to 1986 we splenectomized 181 patients with chronic ITP after platelet kinetic studies with51Cr or111In. Mean age at diagnosis was 34 (range 4–79 yr). Follow‐up of at least 1 yr after splenectomy was available in every patient. 141 patients (78%) achieved remission (platelets>100 times 109/1 by 3 months after splenectomy), of whom 9 subsequently relapsed. Among the 40 non‐responders at 3 months, 3 achieved a later remission spontaneously. Factors associated with response to splenectomy included a high post‐operative platelet count (p = 0.0001), younger age at the time of surgery (p = 0.0077) and predominantly splenic sequestration of platelets (p = 0.0002), the two latter factors being partially correlated. In a multivariate analysis, however, only post‐operative platelet count and age retained an independent prognostic significance, whereas the sequestration site of platelets had only borderline value. These results are discussed in the context of indications of platelet kinetic studies in chronic ITP, before splenectomy is c

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00109.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

18 previously untreated patients with multiple myeloma were entered in a clinical study of a combination chemotherapy, carboquone (CQ) and prednisolone (P) (CQ‐P). Response was observed in 44% of patients treated with CQ‐P judged by the criteria for response to chemotherapy. The median survival time was 33 months. Serious toxic reactions seldom occurred. The results suggest that the combination of CQ‐P has efficacy in the treatment of multiple myeloma and could have a role among primary chemotherapeutic regimens for the disease. Further clinical trials should be conducted to establish a new drug combination in which CQ is inc

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00110.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Tumor necrosis factor (TNF)/cachectin can produce both beneficial and harmful manifestations. Mechanisms may operate to counteract potentially harmful effects such as shock and cachexia. The TNF binding protein (TNF‐BP), which is found at increased levels in serum and urine of patients with chronic renal failure, may play such a role. TNF‐BP was purified 1000000–fold to homogeneity from urine of patients with chronic renal failure by use of ion exchange chromatography, affinity chromatography on TNF‐Sepharose and reverese phase chromatography. The purified protein contained only one chain with an apparent Mron sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of 30000. The aminoterminal amino acid sequence D‐S‐V‐X‐P‐Q‐G‐K‐Y‐I‐H‐P‐Q‐V‐N‐S‐I‐X‐K‐T revealed no significant homologies with previously described protein sequences. TNF‐BP may act as

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00111.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Following indium‐111 oxine‐labelled autologous lymphocyte infusion, 39 lymphocyte scintigraphies were performed in 35 patients with Hodgkin's disease (HD). Lymphocytes were obtained after leukapheresis and Lymphoprep® gradient centrifugation and were further purified by an adherence step to eliminate monocytes. A median of 1.2 (0.2–3.7) × 109cells were labelled with 6.7 (range 1.9–16.6) Mbq indium‐111 oxine and reinfused to the patients. Gamma camera imaging was performed with a GE 400 AT. An accumulation of radioactivity was seen at 54 of 61 sites with enlarged lymph nodes. Increased radioactivity was also seen at 16 sites with no previous clinical evidence of tumour involvement. These data seem very promising and with further methodological improvement lymphoscintigraphy may prove to be an important complement in the staging procedure and follow‐up of pat

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00112.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY