摘要:

A marked elevation of sialyltransferase activity (STA) was observed in a solid tumor of plasma cells, which had been removed from a patient with multiple myeloma (MM), as compared to normal lymphatic tissues. STA was also determined in mononuclear bone marrow cells of 10 patients with MM and found to be 12 times higher than that of bone marrow mononuclear cells from 5 patients with non‐malignant disorders (with less than 1% plasma cells in the bone marrow aspirate). A significant correlation was found between STA and the number of plasma cells in the bone marrow aspirat

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00281.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

ABO‐mismatched bone marrow transplants have resulted in delayed red cell production in patients who have persistently elevated anti‐ABO isohemagglutinin titers. We present a patient with chronic myelogenous leukemia who received an HLA‐matched, ABO‐incompatible bone marrow transplant from his sister. Post‐transplant, he developed pure red cell aplasia with exuberant production ofdonorred cell precursors by in vitro BFU‐E assay. Restriction fragment length polymorphism (RFLP) analysis of bone marrow, peripheral blood and BFU‐E colonies demonstrated only donor type DNA post‐transplant. However, the patient had persistently elevated isohemagglutinin titer and Ph' chromosome‐positive metaphases on chromosome analysis, indicating the presence of persistent host lymphocytes. With onset of acute graft vs. host disease (GVHD), the isohemagglutinin titer dropped, Ph1chromosome‐positive metaphases disappeared, and full hematopoietic recovery ensued. Longitudinal analysis of RFLP's, isohemagglutinin titers and chromosomes may be helpful in understanding the immunological interplay following allogeneic bone mar

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00282.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The erythropoietin level was measured, by bioassay in polycythaemic mice, in the serum of anaemic patients suffering from different types of leukaemia. Comparative measurements were carried out in patients with ulcerative colitis. Serum erythropoietin was less well‐correlated with the haemoglobin concentration in leukaemia than in ulcerative colitis. While serum erythropoietin did no exceed 200 mU/ml in patients with ulcerative colitis (lowest blood haemoglobin concentration 56 g/l), several of the leukaemic patients had serum erythropoietin levels above 500 mU/ml at comparable degrees of anaemia. Bone marrow biopsy showed that erythropoiesis was severely impaired in the leukaemic patients whose erythropoietin values were relatively high. These findings are in accord with the hypothesis that the plasma level of erythropoietin depends not only on the haemoglobin concentration of the blood but also on the bone marrow responsiveness to the hormon

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00283.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

We report studies on vascular endothelial function and ultrastructure in 2 cases of fatal cyclosporin (CS)‐associated thrombotic microangiography following allogeneic bone marrow transplantation (BMT). Spontaneous vascular release of prostacyclin (PGI2) from a vein sample ex vivo was absent, and scanning electron microscopy (SEM) showed surface changes indicative of vascular endothelial damage (case 1). PGI2release from cultured human umbilical vein endothelial cells incubated with patients' serum in vitro was normal in both cases. Plasma von Willebrand factor (vWF) antigen and ristocetin cofactor activity levels were raised in both patients, 5.06 and 7.02 (case 1) and 3.60 and 2.01 (case 2) (normal ranges 0.59–1.57 and 0.42–1.74 U/ml), respectively, but multimer patterns were normal. The SEM appearances coupled with the absent PGI2release and raised vWF levels suggest that vascular endothelial damage is central to the pathogenic process in thrombotic microangiopathy following allogeneic BMT but the mechanisms appear to be distinct from those in the haemolytic uraemic syndrome and de novo thrombotic thrombocytopenic purpura. The precise role of CS in this process remains to be ident

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00284.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

We have investigated whether any prognostic factor can be used to identify those children who have a relapse after discontinuation of therapy for acute lymphoblastic leukemia (ALL). Our population‐based series comprised 167 children with newly diagnosed ALL. The 3‐year event‐free survival rate in these children was 65%. Maintenance therapy was electively discontinued for 120 patients, 20 of whom have subsequently had a relapse 1 to 27 months later. In multivariate analysis the risk of late relapse in the 15 patients with initially enlarged kidneys was 4.5‐fold (95% confidence limits 1.7–11.8) that of the others (p5 × 106/l), but with no blasts in the CSF, was 3.8‐fold (1.5–9.6) that of the others (p<0.01). Our results indicate that enlarged kidneys or abnormal CSF findings at initial diagnosis are associated with an increased risk of late relapse in chi

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00285.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Heme‐microbeads attached themselves to the surface of viable K562 cells in a manner inhibitable by free hemin, indicating heme‐receptor interaction. The microbeads were at first evenly distributed, but after prolonged incubation at 37°C they formed a cap on one pole of the cells indicating clustering of the membrane heme receptors. Membrane proteins were labeled by culturing the cells in the presence of35S‐methio‐nine and were then solubilized with Triton X‐114. The hydrophobic proteins contained about 20% of the total bound label. The solubilized membrane proteins were subsequently adsorbed to a heme‐Sepharose affinity gel. According to SDS‐electrophoresis and subsequent autoradiography, the immobilized heme captures two proteins or a protein with two polypeptides of 20000 and 32000 daltons. The larger of these was only weakly labeled with35S. The same two bands were observed if the cell surface proteins were labeled with125I by the lactoperoxidase method and the subsequently solubilized membrane proteins were isolated with

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00286.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Binding patterns of monoclonal IgM anti‐B, anti‐H and anti‐(Leb+ Y) antibodies on erythrocytes were visualized in the backscatter electron imaging (BEI) mode of a scanning electron microscope by indirect immunogold labelling. The results were independent of secretor status, supporting the view that type 2 oligosaccharides predominate on erythrocytes. There were considerable cell‐to‐cell variations of amounts of the antigens, presumably because of differences of antigen development. The amount of H antigens may have been overestimated in previous studies. B and H antigens seemed linked mostly to mobile structures, probably lipid, on the convex parts of the cells and to immobile structures, probably protein, on the concave parts. Y antigens appeared bound to mobile structures also on the concave part of the cell membrane. The results therefore confirm that B oligosaccharides on erythrocytes develop from both lipid‐ and protein‐bound H, but suggest that Y develops mainly from

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00287.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

In chronic myeloid leukemia (CML) low serum cholesterol is not uncommon and has been linked to the activity of the disease. Despite these observations, most studies concerned with prognostic signs in CML have not included cholesterol. In our study, cholesterol correlated positively with survival (p = 0.0012) and with the duration of chronic phase (p = 0.0059) in a univariate analysis. The multivariate Cox regression model selected cholesterol as a parameter of additive prognostic value in addition to marrow myeloblasts plus promyelocytes, sex, eosinophilia and lactate dehydrogenase.

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00288.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

High doses of melphalan cause severe neutropenia and may irreversibly damage hematopoietic stem cells. Treatment of mice with recombinant murine GM‐CSF (GM‐CSF) for 5 days immediately after 400 μg of melphalan did not prevent the severe neutropenia. However, GM‐CSF accelerated the neutrophil recovery and reduced the mortality rate during the neutropenic period compared to melphalan‐only treated mice. CFU‐GM levels measured 6 d after melphalan treatment without GM‐CSF were markedly reduced in the bone marrow while being elevated in the spleen. In comparison, GM‐CSF further reduced the total CFU‐GM population in melphalan‐treated mice including the levels in the bone marrow and in the spleen. On d 14 after melphalan, the spleen regained its active CFU‐GM production. By d 90, the number of circulating neutrophils, the number of bone marrow CFU‐GM and splenic CFU‐GM were the same in GM‐CSF‐treated and ‐untreated mice. The results suggest that GM‐CSF could be used to shorten the neutropenic period and reduce mortality caused by a high dose of melphalan. Though this effect could be at the expense of a temporary reduction in CFU‐GM population, GM‐CSF did not induce more long‐term damage to myelopoiesis than

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00289.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

When heparin was added to platelet‐rich plasma, mild but irreversible platelet aggregation was demonstrated. This platelet response was not accompanied by release of α‐granules and dense body constituents, nor by prostaglandin biosynthesis. It did, however, require metabolic energy and divalent cations as metabolic inhibitors (anti‐mycin A and 6‐deoxyglucose) and EDTA blocked the reaction. Bernard‐Soulier syndrome platelets, which lack glycoprotein (GP) Ib, but not Glanzmann's Thrombasthenia platelets, which lack GP IIb/IIIa, were aggregated by heparin. Monoclonal antibody (mAb) against GP IIb/IIIa, but not mAb against GP Ib, strongly inhibited the reaction. These combined results suggest the participation of GP IIb/IIIa but not GP Ib in heparin‐induced platelet aggregation. Fibrinogen was a cofactor in the reaction as gel‐filtered platelets were unreactive to heparin but addition of fibrinogen restored their reactivity. Antithrombin III and fibronectin inhibited platelet response to heparin, suggesting that these proteins may protect platelets from aggregat

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1989.tb00290.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY