摘要:

Abstract:In an ongoing phase I1 study, 12 patients with lymphoma and HIV infection were treated with zidovudine (ZDV) and recombinant interleukin‐2 (rIL‐2) to evaluate if this association may produce beneficial effect on the immunologic status and the outcome of lymphoma. The protocol included daily doses of rIL‐2 at 6 MIU/m2over 5 days in c.i. per week for a total 4 courses; ZDV was associated at 600 mg/d in the period under study. An improved CD4 count, exceeding 2‐ to 4‐fold the basal count, was obtained in patients with a basal CD4 number>100/μl accompanied by a significant increase of NK and LAK activity (p100/μl obtained a complete remission after rIL‐2 and ZVD. The p24 antigen, taken as parameter of viral replication, remained invariably negative after rIL‐2 and ZDV in patients already negative and became negative in 1 patient previously positive. Our conclusion is that the association of rIL‐2 and AZT is safe and useful in patients with lymphoma

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1992.tb00905.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:Although DDAVP has been shown to be haemostatically efficacious in patients with various congenital or acquired platelet disorders, no reasonable explanation has been found for this effect. We have previously shown DDAVP to increase platelet adhesiveness as measured with a platelet retention test. The aim of the present study was to investigate the mechanism of action responsible for the increased platelet retention in response to DDAVP. Patients with vWD type III and type Ia, severe haemophilia and severe thrombasthenia, as well as healthy controls, were included in the study. The effect of different concentrations of vWF in plasma and platelets was explored, as was the effect on platelet function of apyrase and monoclonal antibodies against GP IIb/IIIa and GP Ib. We found the effect of DDAVP on platelet retention to be unaffected by changes in the plasma concentration of vWF. The enhanced platelet retention after DDAVP is apparently dependent on the presence of platelet‐vWF and on a normal function of the GP IIb/IIIa. The effect is not mediated via ADP or thrombin. The platelet‐stimulating effect of DDAVP may be one explanation for the positive haemostatic effect in patients with certain platelet disord

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1992.tb00906.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:We evaluated the activation sequence of peripheral blood lymphocytes from healthy donors using different mitogens, including antilymphocyte globulin (ALG), antLCD3 monoclonal antibody (OKT3), and phytohemagglutinin (PHA). Blood mononuclear cells stimulated by ALG, OKT3 and PHA incorporated 3H‐thymidine in the same way. When enriched T cells were tested in the presence of interleukin‐lα (0 to 100 Ujml), incorporation of3H‐thymidine was greater in those cells stimulated by ALG than by PHA. OKT3 did not activate enriched T cells. Thymidine incorporation was reduced to less than 50% of maximum concentrations by the addition of 10‐7mol/1,25‐dihydroxyvitamin D3(vit D3) in PHA‐ or OKT3‐activated cells. However, the inhibitory effect of vit D3was not apparent in ALG‐activated cells. Production of granulocyte‐macrophage colony‐stimulating factor and interleukin‐3 by lymphocytes upon activation was consistently higher when cells were treated with ALG or PHA than with OKT3. Taken together, the data indicate that there appear to be distinct functional mechanisms between ALG‐ and OKT3‐induced lymphocyte activation that lead to characteristi

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1992.tb00907.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:Recombinant human granulocyte colony‐stimulating factor (rhG‐CSF) was administered (1.5 pg/kg body weight) subcutaneously once daily for 5 to 9 days to 5 patients with malignant lymphoma. In all patients, initial administration of rhG‐CSF induced a rapid fall in the neutrophil count within 30 minutes, followed by a recovery and an increase in the neutrophil count within 150 min. A rapid fall in the neutrophil count was accompanied by increased expression of neutrophil C3bi‐receptors, and neutrophils left in the circulation had lower activity of neutrophil alkaline phosphatase (NAP) and phagocytosis. A decrease in the NAP scores observed at 30 min reflected a preferential decrease of neutrophils with high NAP activity. A recovery and an increase in the neutrophil count were accompanied by a further decrease of NAP scores, which was caused by a preferential increase of neutrophils with lower NAP activity. The NAP scores of mature neutrophils from peripheral blood were not affected byin vitrotreatment of cells with rhG‐CSF for up to 150 min at 37 °C. These findings and the previous observations that neutrophils in the circulating and marginal pools have high NAP activity and neutrophils in the bone marrow pool have low NAP activity taken together suggest that, following initial administration of rhG‐CSF, functionally active neutrophils leave the bloodstream preferentially, which is primarily followed by an influx of neutrophils from the bone marrow, but not by demargination of sequestered

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1992.tb00908.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:The aim of the present study was to investigate how four different filters, i.e. Imugard IG500 (Terumo, Japan), Miropore (Miramed, Italy), Pall P1‐100 (Pall, USA) and Sepacell P1‐10A (Asahi, Japan) affect some biochemical properties of platelet concentrates. The work was conducted using 42 pairs of platelet Concentrates. After 2 days of storage, one of the preparations was filtered and the other served as an unfiltered control. Immediately after filtration, determination of the platelet count, desarginated activated complement factor 3 (C3a des arg) and the extracellular and total concentrations of platelet factor 4 (PF4) and lactate dehydrogenase (LDH) were carried out on both these platelet concentrates. After an additional storage period of 3 d, extracellular concentrations of PF4 and LDH were determined on both concentrates. A significant decrease of extracellular PF4 concentration was found immediately after filtration when Pall P1‐100 and Imugard IG500 were used. During the 3‐d storage after filtration, the concentrates filtered with Imugard IG500 and Pall P1‐100 demonstrated significantly higher platelet lysis as compared to the unfiltered controls. It is concluded that the present work demonstrates storage instability after filtration with Imugard IG500 and Pall P1‐100. Therefore, platelet concentrates filtered with these filters would not appear to be suitable

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1992.tb00909.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:Differences in the plastic adhesive properties of bone marrow (BM) cells were used to initiate modified stromal layers (MSL) from long‐term cultures by removing non‐adherent cells shortly (4 to 18 hours) after initial seeding. Following this early modification, adherent cells generated a confluent layer after 21 days of culture. Cellular characteristics of volume and spontaneous fluorescence determined by flow cytometry showed that the MSL included 82% fibroblastic stromal cells, 8% macrophages and 10% myelomonocytic cells. Furthermore, clonogenic assays revealed that the MSL were devoid of hematopoietic progenitor cells. MSL were found to sustain long‐term myelopoiesis for at least 7 weeks from exogenously added hematopoietic progenitors isolated from bone marrow (CD34+ cells), thereby demonstrating their functionality. The present experimental model appears of interest for the study of interactions between defined populations of hematopoietic cells and cells of the adherent layer. Of importance, our present modifications of human long‐term bone marrow culture are technically simple and do not involve manipulation of the stroma

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1992.tb00910.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

摘要:

Abstract:Evidence is presented for the existence of a serum factor(s) (SF), which inhibits the growth of both the interleukin‐2 (IL‐2)‐dependent cell line CTLL and the 2‐day generation of CTL cells. This activity is found in the serum of both nude and euthymic mice and its suppressive effect can be detected about 18 hours after addition to CTLL cultures. The inhibitory activity elutes from a Sepharose 6B gel after the gamma globulin fraction (100–150 kD), and is precipitated by ammonium sulfate at 60 wjv % saturation. IL‐3‐mediated bone marrow colony formation is not inhibited by SF. It also does not suppress the growth of a panel of different tumor cell lines. The spleen cell responsiveness to both Con A and LPS activation is greatly reduced in the presence of SF. However, binding of radiolabelled IL‐2 to CTLL cells was not blocked by SF, although the activity was greatly reduced by absorption to these cells. Our data support the existence of factor(s) in sera that may have a regulatory role on IL‐2

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1992.tb00911.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1992.tb00912.x

出版商:Blackwell Publishing Ltd    

年代:1992

数据来源: WILEY