ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1993.tb00144.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract: We compared three methods of detecting platelet antigens for antiplatelet antibodies in patients with idiopathic thrombocytopenic purpura (ITP), i.e., a microtiter well antigen‐capture enzyme‐linked immunosorbent assay (AC‐ELISA), a platelet suspension immunofluorescence test using flow cytometry (PSIFT‐FCM), and Western blotting. Using PSIFT‐FCM, the reactivity of NNKY1–32, an anti‐glycoprotein (GP) IIb/IIIa antibody, and of NNKY5‐5 (anti‐GPIb) to platelets from 60 ITP patients were examined. By PSIFT‐FCM, both the peak channel and the relative fluorescence value were below the mean‐2SD for healthy control platelets in 15 patients when NNKY1–32 was used and in 2 patients when NNKY5‐5 was used. Western blotting gave an apparent molecular weight for GPIb of 160000, while GPIIb was 135000 and GPIIIa was 88000. By the AC‐ELISA, 12 patients were positive for NNKY1–32 and 4 for NNKY5‐5. Although NNKY1‐32 binding was detected by PSIFT‐FCM in 15 of the ITP patients using platelets, only 3 were positive using plasma. By AC‐ELISA and Western blotting of plasma, 12 and 10 of the patients were positive for NNKY1–32 and NNKY5‐5, respectively. Our results suggest that none of the three methods is good enough to stand alone and that they should be used together in the analysis of platelet

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1993.tb00145.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract: The camptothecin derivatives 9‐nitro‐camptothecin (9NC) and 9‐amino‐camptothecin (9AC) inhibit similarly growth of HL‐60, KG‐1, and U‐937cells in vitro, whereas growth of THP‐1 cells is inhibited by 9AC, but not by 9NC. Growth inhibition is accompanied by enlargement of cells which contain one (HL‐60, THP‐1) or more (KG‐1, U‐937) nuclei. Flow cytometry studies showed that 9NC‐treated HL‐60 and U‐937 cells accumulate in the S and G2phases of the cell cycle; then they die by apoptosis, with the HL‐60 cells being more sensitive than U‐937 cells to 9NC. In contrast, 9NC‐treated KG‐1 and THP‐1 cells accumulate in S and G2phases, but resist death by apoptosis. Of the cell lines tested, only U‐937 cells xenografted in nude mice generated subcutaneous myeloid tumors, which exhibited a delayed growth in the presence of 9NC. Further, 9NC‐treated advanced U‐937 tumors regressed temporarily, indicating that U‐937 cells consi

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1993.tb00146.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract: The pathogenesis of anaemia associated with human immunodeficiency virus infection is still far from being understood. It cannot be explained by direct effects of the virus on the haematopoietic system. Recent data suggest a role for immune activation. In a cross‐sectional study we compared blood cell counts, haemoglobin and erythropoietin levels of 63 HIV‐seropositive individuals with immune activation markers (interferon‐γ, serum and urine neopterin, and β2‐microglobulin) and with parameters or iron metabolism (serum iron, transferrin, free iron binding capacity, ferritin). We found significant correlations between the concentrations of haemoglobin and the immune activation markers and erythropoietin concentrations. Additional significant correlations existed between the parameters of iron metabolism and haemoglobin levels, and ferritin correlated inversely with transferrin. In sum, low haemoglobin levels in patients were associated with enhanced cellular immune activation, as seen by increased interferon‐γ, neopterin and β2‐microglobulin, and with changes of iron metabolism: low haemoglobin was associated with low transferrin and free iron binding capacity and high ferritin levels. Endogenous release of cytokines such as interferon‐γ‐inhibiting crythropoiesis may be one underlying cause of ana

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1993.tb00147.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract: From October 1983 until December 1988, 50 patients with asymptomatic multiple myeloma stage I were included in a prospective randomized multi‐centre study comparing melphalan‐prednisone (MP) therapy started at the time of diagnosis with deferred therapy where MP was started at the time of disease progression. Twenty‐five patients were randomized to each group. The median time from diagnosis to start of therapy in the group with deferred therapy was 12 months. The reasons for starting therapy were increasing M‐protein in 8 cases, symptomatic bone disease in 9 and anaemia in 5. In 2 cases, disease progression was complicated by vertebral fractures necessitating radiotherapy. Two patients in the group in which MP was started at the time of diagnosis developed acute leukaemia. No differences in response rate, response duration or survival were observed between the treatment groups. We conclude that in asymptomatic myeloma deferral of chemotherapy is feasible in well‐informed and well‐controlled patients but conveys no advantage in survival. In clinical practice the benefits of treatment deferral are to some extent outweighed by disease progression before start

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1993.tb00148.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract: A leukemic phase occurred in 7 of 11 (64%) Japanese patients with follicular lymphoma. The clinical and hematologic features at the onset of this phase were splenomegaly, anemia, and thrombocytopenia. The lymphoma cells expressed monoclonal surface immunoglobulins with moderate to strong intensity in all 7 of the patients diagnosed as leukemic. Various B‐cell associated antigens were expressed as follows: CD19 (5/6), CD20 (7/7), and CD 10 (6/7). The reactivity to these markers was comparable in the lymph node and blood samples. The expression of CD38 antigen was much lower in the lymphoma cells of the blood than in those of the lymph nodes. Cytogenetic studies of the lymph nodes of follicular lymphoma in leukemic phase revealed a common chromosomal aberration, of t(14;18)(q32;q21) and + 18, in 2 patients successfully analyzed. Although the follicular lymphomas in the leukemic phase in these patients in Akita, Japan, were consistent with those in the West with respect to morphology, immunology and cytogenetics, the high incidence of leukemic manifestations may be a salient feature of Japanese ca

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1993.tb00149.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract: A prospective controlled trial was performed to determine whether the use of ABO‐identical platelets from the start of treatment might provide higher post‐transfusion platelet increments, reduce the number of platelet transfusions and ultimately delay the onset of refractoriness. Forty newly diagnosed patients with haematological diseases were randomized to receive either pooled ABO‐identical platelets or pooled platelets unmatched for ABO group throughout their course. The corrected platelet count increments (CCI) were calculated for the first 25 transfusions of each patient and non‐immune factors present at the time of each platelet transfusion were documented. The mean CCI for the first 25 transfusions in the ABO‐identical group was significantly higher (6600 ±: 7900 SD) than that achieved with ABO unmatched platelets (5200 ±: 7900; p<0.01). The effect was most marked for the first 10 transfusions for each patient where the CCI was 64% higher in the ABO‐identical group (8200 ±: 7500 vs 5000 ±: 8100; p<0.0002). Patients given ABO‐identical platelets required only about half as many transfusions in the first 30 days (10 versus 17, p<0.05) or during the first admission (11 versus 21 p<0.01) as patients in the ABO‐unmatched group. A smaller percentage of patients in the ABO‐identical group became refractory (36% vs 75% p<0.03). The data suggest that patients requiring long‐term platelet support should be transfused wit

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1993.tb00150.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

摘要:

Abstract: The purpose of this study was to determine the radioprotective ability of primitive hematopoietic precursors which form colonies in diffusion chambers in mice (CFU‐D). Thirty‐two lethally irradiated female ICR mice were injected with 5 to 7 male ICR mouse bone marrow‐derived CFU‐D colonies each. Fourteen of these mice survived over 30 days and were sacrificed at intervals up to a year. As a control, 20 lethally irradiated female ICR mice received cells from intercolony areas. All of these mice died before day 20. DNA samples obtained from hematopoietic organs and liver from 8 sacrificed mice were analyzed for the presence of CFU‐D colony‐derived cells. Only in 1 ICR mouse was CFU‐D colony origin DNA detected by Southern analysis in all hematopoietic organs: bone marrow, spleen, thymus and lymph nodes. In 6 mice, only selected hematopoietic organs were repopulated by CFU‐D colony‐derived cells as judged by Southern analysis. In some of these mice, the remaining hematopoietic organs contained small CDU‐D‐derived cell populations which could be detected by more sensitive polymerase chain reaction (PCR). In 1 mouse, the presence of CFU‐D‐derived cells in all hematopoietic organs was only demonstrated by PCR. These findings suggest that lethally irradiated mice can be rescued by CFU‐D‐derived daughter cells. They appear to have the potential to give rise to clones containing lymphoid and myeloid cells in all hematopoietic organs, at least temporarily. Thus, it can be concluded that CFU‐D represents a very primitive hematopoietic precursor cel

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1993.tb00151.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1993.tb00152.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY

ISSN:0902-4441    

DOI:10.1111/j.1600-0609.1993.tb00153.x

出版商:Blackwell Publishing Ltd    

年代:1993

数据来源: WILEY